公开(公告)号：EP3231868A4

公开(公告)日：2018-05-23

申请号：EP15867235

申请日：2015-09-07

申请人： INST OF BIOTECHNOLOGY ACADEMY OF MILITARY MEDICAL SCIENCES CHINA

发明人： WU JUN ,  SUN PENG ,  LIU BO ,  CHANG SHAOHONG ,  GONG XIN ,  WANG HENGLIANG ,  ZHU LI ,  PAN CHAO ,  FENG ERLING

IPC分类号： C12N15/11 ,  A61K48/00 ,  A61P35/00 ,  C12N15/63

CPC分类号： A61K39/0275 ,  A61K48/00 ,  A61K2039/521 ,  A61K2039/523 ,  C12N9/1051 ,  C12N9/93 ,  C12N15/11 ,  C12N15/63 ,  C12N15/74 ,  C12Y204/01 ,  C12Y600/00 ,  Y02A50/472 ,  Y02A50/482

摘要： The present invention discloses a Salmonella Paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cld LT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB4573 3 -OPS Spty 50973 and rEPA4573-OPS Spty 50973 as prepared by using Salmonella Paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.

公开(公告)号：EP3225690A4

公开(公告)日：2018-06-20

申请号：EP15862847

申请日：2015-09-01

申请人： INST OF BIOTECHNOLOGY ACADEMY OF MILITARY MEDICAL SCIENCES CHINA

发明人： WU JUN ,  PAN CHAO ,  SUN PENG ,  WANG HENGLIANG ,  LIU BO ,  PENG ZHEHUI ,  ZHU LI ,  CHANG SHAOHONG ,  GONG XIN ,  FENG ERLING ,  WANG BIN ,  ZENG MING

IPC分类号： C12N15/70 ,  A61K39/08 ,  A61K39/104 ,  A61K39/112 ,  A61P31/04 ,  C07K19/00 ,  C12N15/74

CPC分类号： A61K39/08 ,  A61K39/104 ,  C07K19/00 ,  C12N15/70 ,  C12N15/74 ,  Y02A50/472 ,  Y02A50/476 ,  Y02A50/482

摘要： The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and the Neisseria meningitidis O-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.