公开(公告)号：EP2123668A1

公开(公告)日：2009-11-25

申请号：EP08290476.4

申请日：2008-05-22

申请人： INSTITUT PASTEUR

发明人： Mulard, Laurence ,  Phalipon, Armelle ,  Vulliez-Le Normand, Brigitte ,  Theillet, François-Xavier ,  Bentley, Graham ,  Saul, Frederick ,  Delepierre, Murielle ,  Felici, Franco

IPC分类号： C07K11/00 ,  C07K14/00 ,  A61K39/112 ,  A61P31/04

CPC分类号： G01N33/56916 ,  A61K39/00 ,  A61K2039/53 ,  C07K14/25 ,  C07K16/1228 ,  C07K2317/34 ,  C07K2317/55 ,  G01N2333/25 ,  G01N2400/50 ,  Y02A50/476

摘要： The present invention is directed to purified immunogenic polypeptides comprising an epitope unit recognized by a protective monoclonal antibody having a high affinity and a high specificity for the O-antigen from serotype 2a Shigella flexneri. The invention also concerns the use of said purified immunogenic polypeptides for the preparation of vaccine against serotype 2a S . flexneri infection and for the preparation of diagnostic compositions for the detection of serotype 2a S . flexneri infection. Finally, the present invention comprises high-resolution crystal structures of said natural O-antigen, or functional fragments thereof, possibly synthetic, and a the mimetic peptide, in complex with a protective monoclonal antibody, or functional fragments thereof, and to methods for selecting immunogenic polypeptides for the preparation of new candidate vaccinal compositions based on said crystals.

摘要翻译： 本发明涉及纯化的免疫原性多肽，其包含由具有高亲和力和从血清型2A痢疾杆菌的O-抗原具有高特异性的保护性单克隆抗体的表位识别的单元的。 因此，本发明涉及使用。所述的纯化免疫原性多肽在制备疫苗的针对血清型2A S. 菌感染和用于制备诊断组合物的用于检测血清型的图2a S. 菌感染。 最后，本发明包括所述天然O-抗原的高分辨率晶体结构，或其功能性片段，可能合成的，并且在一个复杂的模拟肽具有保护单克隆抗体，或其功能性片段，以及一种用于选择方法 免疫原性多肽的用于基于所述晶体新的候选疫苗组合物的制备。