公开(公告)号：EP1456386A2

公开(公告)日：2004-09-15

申请号：EP02802572.4

申请日：2002-11-18

申请人： Idec Pharmaceuticals Corporation

发明人： REFF, Mitchell ,  BARNETT, Richard

IPC分类号： C12N15/63 ,  C12N15/12 ,  C12N15/85 ,  C12N15/86 ,  C12N15/00 ,  C07K16/00

CPC分类号： A61K51/1045 ,  A61K47/6849 ,  A61K47/6851 ,  A61K47/6869 ,  A61K47/6901 ,  A61K51/1027 ,  A61K51/1072 ,  C07K16/00 ,  C07K16/2851 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/52 ,  C12N15/85 ,  C12N2800/108 ,  C12N2840/20 ,  C12N2840/203 ,  C12P21/02

摘要： Described herein is a novel expression system for producing multiple gene products of interest from a single polycistronic construct. In particular, the expression system contains a polycistronic vector capable of expressing functional antibodies in eukaryotic host cells, which vector contains at least the following elements operably linked in the 5' to 3' orientation: a promoter operable in a eukaryotic cell; a DNA sequence encoding at least the variable region of an antibody light chain; an internal ribosome entry site (IRES); and at least one DNA sequence encoding an antibody heavy chain. Also disclosed are mammalian cells containing the polycistronic expression vector, and a method of producing functional antibodies in mammalian cells transfected with the polycistronic expression vector.

公开(公告)号：EP1414948A2

公开(公告)日：2004-05-06

申请号：EP02742400.1

申请日：2002-07-10

申请人： Idec Pharmaceuticals Corporation

发明人： REFF, Mitchell ,  BETENBAUGH, Michael, J. ,  FIGUEROA, Bruno, Jr. ,  AILOR, Eric ,  HARDWICK, Marie

IPC分类号： C12N5/10 ,  C12N5/06 ,  C12N5/08 ,  C12P21/00 ,  C07H21/00 ,  C07K14/00

CPC分类号： C07K14/4747 ,  A61K39/00 ,  A61K48/005 ,  C07K14/721 ,  C07K16/2875 ,  C07K2319/00 ,  C12N15/67 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2840/20 ,  C12N2840/44

摘要： The present invention relates to preventing or delaying programmed cell death by expressing one or more anti-apoptotic polypeptides in a cell such that programmed cell death in the cell is prevented or delayed. The present invention also relates to increasing production of a cell-related product by expressing one or more anti-apoptotic polypeptides in a cell such that production of the cell-related product by the cell is increased. Recombinant cells useful for producing cell-related product or cellular therapy are also provided.