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1.发明公开
公开(公告)号：EP3384046A1
公开(公告)日：2018-10-10
申请号：EP16816512.4
申请日：2016-11-30
申请人： Illumina, Inc.
发明人： JAMSHIDI, Arash , LIN, Yan-you , ABSALAN, Farnaz , STUART, Sarah , CANN, Gordon , WU, Yir-Shyuan , KHURANA, Tarun , FISHER, Jeffrey, S.
CPC分类号： B01L3/502761 , B01L3/502784 , B01L3/5088 , B01L2200/0605 , B01L2200/0642 , B01L2200/0668 , B01L2300/0819 , B01L2300/165 , B01L2400/0427 , B01L2400/043 , C12Q1/6806 , C12Q1/6869 , C12Q2563/143 , C12Q2563/149 , C12Q2563/185 , G01N1/28 , G01N33/54333 , G01N2015/1006 , C12Q2535/122
摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
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公开(公告)号：EP3172321B1
公开(公告)日：2019-08-21
申请号：EP15753229.2
申请日：2015-07-20
申请人： Illumina, Inc.
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公开(公告)号：EP3172321A1
公开(公告)日：2017-05-31
申请号：EP15753229.2
申请日：2015-07-20
申请人： Illumina, Inc.
发明人： CANN, Gordon , MANDELL, Jeffrey G. , ARAVANIS, Alex , NORBERG, Steven , POKHOLOK, Dimitry K. , STEEMERS, Frank. J. , ABSALAN, Farnaz , BAZARGAN, Leila
CPC分类号： C12P19/34 , C12N15/102 , C12Q1/6816 , C12Q1/683 , C12Q1/6869 , C12Q2521/301 , C12Q2522/101 , C12Q2563/131
摘要： A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.
摘要翻译： 用于富集靶核酸的方法包括提供具有crRNA或其衍生物和Cas蛋白或其变体的核酸内切酶系统。 crRNA或其衍生物包含与靶核酸的区域基本上互补的靶特异性核苷酸区域; 使靶核酸与内切核酸酶系统接触以形成复合物; 并分离复合物并由此富集靶核酸。
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4.发明公开
公开(公告)号：EP3907295A1
公开(公告)日：2021-11-10
申请号：EP21170333.5
申请日：2016-11-30
申请人： Illumina, Inc.
发明人： JAMSHIDI, Arash , LIN, Yan-you , ABSALAN, Farnaz , STUART, Sarah , CANN, Gordon , WU, Yir-Shyuan , KHURANA, Tarun , FISHER, Jeffrey S.
摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
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