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1.发明公开LIBRARY PREPARATION OF TAGGED NUCLEIC ACID USING SINGLE TUBE ADD-ON PROTOCOL 审中-公开TAGGED NUCLEIC图书馆使用的生产add添加协议与单管
公开(公告)号：EP3161137A1
公开(公告)日：2017-05-03
申请号：EP15744405.0
申请日：2015-06-25
申请人： Illumina, Inc.
发明人： KAPER, Fiona , CANN, Gordon
摘要： A method of preparing a library of tagged nucleic acid fragments including contacting a population of cells directly with a lysis reagent having one or more protease to generate a cell lysate; inactivating the protease to generate an inactivated cell lysate, and applying a transposase and a transposon end composition containing a transferred strand to the inactivated cell lysate under conditions wherein the target nucleic acid and the transposon end composition undergo a transposition reaction.
摘要翻译： 制备包括与具有一种或多种蛋白酶，以产生细胞裂解物裂解试剂直接接触的细胞群标记核酸片段的文库的方法; 灭活蛋白酶，灭活的细胞裂解物，以产生，并施加转座和转座子末端组合物包含A输送到条件下海滩灭活细胞裂解物worin靶核酸和转座子末端组合物经历转座反应。
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公开(公告)号：EP3680333A1
公开(公告)日：2020-07-15
申请号：EP20154378.2
申请日：2015-04-28
申请人： Illumina, Inc.
发明人： KAPER, Fiona , FAN, Jian-Bing , SALATHIA, Neeraj , CANN, Gordon , JAMSHIDI, Arash , ARAVANIS, Alex
IPC分类号： C12N15/10
摘要： Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).
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3.发明公开
公开(公告)号：EP3384046A1
公开(公告)日：2018-10-10
申请号：EP16816512.4
申请日：2016-11-30
申请人： Illumina, Inc.
发明人： JAMSHIDI, Arash , LIN, Yan-you , ABSALAN, Farnaz , STUART, Sarah , CANN, Gordon , WU, Yir-Shyuan , KHURANA, Tarun , FISHER, Jeffrey, S.
CPC分类号： B01L3/502761 , B01L3/502784 , B01L3/5088 , B01L2200/0605 , B01L2200/0642 , B01L2200/0668 , B01L2300/0819 , B01L2300/165 , B01L2400/0427 , B01L2400/043 , C12Q1/6806 , C12Q1/6869 , C12Q2563/143 , C12Q2563/149 , C12Q2563/185 , G01N1/28 , G01N33/54333 , G01N2015/1006 , C12Q2535/122
摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
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公开(公告)号：EP3172321B1
公开(公告)日：2019-08-21
申请号：EP15753229.2
申请日：2015-07-20
申请人： Illumina, Inc.
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公开(公告)号：EP3172321A1
公开(公告)日：2017-05-31
申请号：EP15753229.2
申请日：2015-07-20
申请人： Illumina, Inc.
发明人： CANN, Gordon , MANDELL, Jeffrey G. , ARAVANIS, Alex , NORBERG, Steven , POKHOLOK, Dimitry K. , STEEMERS, Frank. J. , ABSALAN, Farnaz , BAZARGAN, Leila
CPC分类号： C12P19/34 , C12N15/102 , C12Q1/6816 , C12Q1/683 , C12Q1/6869 , C12Q2521/301 , C12Q2522/101 , C12Q2563/131
摘要： A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.
摘要翻译： 用于富集靶核酸的方法包括提供具有crRNA或其衍生物和Cas蛋白或其变体的核酸内切酶系统。 crRNA或其衍生物包含与靶核酸的区域基本上互补的靶特异性核苷酸区域; 使靶核酸与内切核酸酶系统接触以形成复合物; 并分离复合物并由此富集靶核酸。
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6.发明公开
公开(公告)号：EP3907295A1
公开(公告)日：2021-11-10
申请号：EP21170333.5
申请日：2016-11-30
申请人： Illumina, Inc.
发明人： JAMSHIDI, Arash , LIN, Yan-you , ABSALAN, Farnaz , STUART, Sarah , CANN, Gordon , WU, Yir-Shyuan , KHURANA, Tarun , FISHER, Jeffrey S.
摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
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公开(公告)号：EP3161137B1
公开(公告)日：2020-05-13
申请号：EP15744405.0
申请日：2015-06-25
申请人： Illumina, Inc.
发明人： KAPER, Fiona , CANN, Gordon
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