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公开(公告)号：EP2279263B1
公开(公告)日：2013-09-04
申请号：EP09739895.2
申请日：2009-04-30
IPC分类号： C12Q1/68
CPC分类号： C12N9/96 , C12Q1/6844 , C12Q1/6853 , C12Q2549/101 , C12Q2525/186 , C12Q2521/301 , C12Q2525/121
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公开(公告)号：EP3380635A2
公开(公告)日：2018-10-03
申请号：EP16816497.8
申请日：2016-11-25
发明人： ROSE, Scott , BELTZ, Kristin , RETTIG, Garrett , CHEN, Caifu , DOBOSY, Joseph , TSANG, Pak Wah , BEHLKE, Mark Aaron , BAO, Yun , WANG, Yu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2525/121 , C12Q2525/131 , C12Q2525/186
摘要： The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays.
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公开(公告)号：EP3150727A1
公开(公告)日：2017-04-05
申请号：EP16198184.0
申请日：2009-04-30
IPC分类号： C12Q1/68
CPC分类号： C12N9/96 , C12Q1/6844 , C12Q1/6853 , C12Q2549/101 , C12Q2525/186 , C12Q2521/301 , C12Q2525/121
摘要： The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3' end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.
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公开(公告)号：EP4267762A1
公开(公告)日：2023-11-01
申请号：EP21848391.5
申请日：2021-12-22
发明人： DOBOSY, Joseph , FROEHLIG, John , PERSCHBACHER, Katherine , BELTZ, Kristin , ROSE, Scott , BEHLKE, Mark Aaron
IPC分类号： C12Q1/6853 , C12N15/62
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公开(公告)号：EP3102676A1
公开(公告)日：2016-12-14
申请号：EP14821405.9
申请日：2014-12-09
发明人： ALLEN, Shawn , BELTZ, Kristin , ROSE, Scott
CPC分类号： C12N15/1031 , C12N15/10 , C12N15/102 , C12N15/1068 , C12N15/66
摘要： This invention pertains to improved methods for the synthesis of long, double stranded nucleic acid sequences containing difficult to clone or variable regions.
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公开(公告)号：EP4437111A2
公开(公告)日：2024-10-02
申请号：EP22839592.7
申请日：2022-11-21
IPC分类号： C12N15/115 , C12N9/12 , C12Q1/6848
CPC分类号： C12N15/115 , C12Q1/6848 , C12N9/1252 , C12Q1/686
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公开(公告)号：EP2553123B1
公开(公告)日：2016-08-24
申请号：EP11760380.3
申请日：2011-03-28
发明人： ROSE, Scott , BEHLKE, Mark A. , OWCZARZY, Richard , WALDER, Joseph A. , THOMAS, Derek M. , MARVIN, Michael R.
CPC分类号： C12Q1/6832 , C07H21/04 , C12Q1/6818 , C12Q1/6851 , G01N33/5308 , G01N33/542 , C12Q2525/185 , C12Q2525/197 , C12Q2565/101
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公开(公告)号：EP2553123A1
公开(公告)日：2013-02-06
申请号：EP11760380.3
申请日：2011-03-28
发明人： ROSE, Scott , BEHLKE, Mark A. , OWCZARZY, Richard , WALDER, Joseph A. , THOMAS, Derek M. , MARVIN, Michael R.
CPC分类号： C12Q1/6832 , C07H21/04 , C12Q1/6818 , C12Q1/6851 , G01N33/5308 , G01N33/542 , C12Q2525/185 , C12Q2525/197 , C12Q2565/101
摘要： A composition comprising an oligonucleotide having the structure 5′-Y1-L1-X-L2-Y2-3′. Y1 comprises a sequence of one or more DNA or RNA nucleotides, including a first nucleotide N1 having a 3′ phosphate covalently linked to L1. Y2 comprises a sequence of one or more DNA or RNA nucleotides, including a second nucleotide N2 having a 5′ phosphate covalently linked to L2. L1 and L2 each independently are a direct bond or a C1-C7 alkyl, alkynyl, alkenyl, heteroalkyl, substituted alkyl, aryl, heteroaryl, substituted aryl, cycloalkyl, alkylaryl, or alkoxyl group. X is R1 is a hydrogen or a C1-C8 alkyl. M is a label or ligand comprising a fused polycyclic aromatic moiety.
摘要翻译： 包含具有结构5'-Y1-L1-X-L2-Y2-3'的寡核苷酸的组合物。 Y1包含一个或多个DNA或RNA核苷酸的序列，包括具有3'端的第一核苷酸N1， 磷酸与L1共价连接。 Y2包含一个或多个DNA或RNA核苷酸的序列，包括具有5'端的第二核苷酸N2， 磷酸酯与L2共价连接。 L 1和L 2各自独立地为直接键或C 1 -C 7烷基，炔基，烯基，杂烷基，取代的烷基，芳基，杂芳基，取代的芳基，环烷基，烷基芳基或烷氧基。 X是R 1是氢或C 1 -C 8烷基。 M是包含稠合多环芳族部分的标记或配体。
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公开(公告)号：EP2279263A2
公开(公告)日：2011-02-02
申请号：EP09739895.2
申请日：2009-04-30
IPC分类号： C12Q1/68
CPC分类号： C12N9/96 , C12Q1/6844 , C12Q1/6853 , C12Q2549/101 , C12Q2525/186 , C12Q2521/301 , C12Q2525/121
摘要： The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3' end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.
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