专利检索 ap:("Introgene B.V.") AND inv:"UYTDEHAAG, Alphonsus, Gerardus, Cornelis, Maria" 第 1 页

1.

发明公开
RECOMBINANT PROTEIN PRODUCTION IN A HUMAN CELL USING SEQUENCES ENCODING ADENOVIRUS E1 PROTEIN 有权
标题翻译：使用腺病毒E1蛋白序列编码，重组蛋白的人类细胞中的生产。

公开(公告)号：EP1161548A2

公开(公告)日：2001-12-12

申请号：EP00921175.6

申请日：2000-04-17

申请人： Introgene B.V.

发明人： HATEBOER, Guus , VERHULST, Karina, Cornelia , SCHOUTEN, Govert, Johan , UYTDEHAAG, Alphonsus, Gerardus, Cornelis, Maria , BOUT, Abraham

IPC分类号： C12N15/85 , C07K14/505 , C12N15/86

CPC分类号： C07K14/505 , C12N7/00 , C12N15/85 , C12N2510/02 , C12N2510/04 , C12N2710/10343 , C12N2800/108 , C12N2830/00 , C12N2830/15 , C12N2830/60 , C12N2840/20

摘要： The present invention provides methods and compositions for the production of recombinant proteins in a human cell line, using sequences encoding at least one E1 protein of an adenovirus where the cells does not encode a structural adenoviral protein from its genome. The methods and compositions are particularly useful for generating stable expression of human recombinant proteins of interest that are modified post-translationally, e.g. by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems like Chinese Hamster Ovary (CHO) cells.