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    MEANS AND METHODS FOR NUCLEIC ACID TRANSFER
    1.
    发明公开
    MEANS AND METHODS FOR NUCLEIC ACID TRANSFER 审中-公开
    手段与方法运输的NUCLEIC

    公开(公告)号：EP1198559A1

    公开(公告)日：2002-04-24

    申请号：EP00925728.8

    申请日：2000-04-25

    申请人： Introgene B.V. ,  RIJKSUNIVERSITEIT LEIDEN

    发明人： BOUT, Abraham ,  VALERIO, Domenico ,  SCHOUTEN, Govert, Johan ,  FALLAUX, Frits, Jacobus ,  HOEBEN, Robert, Cornelis ,  VAN DER EB, Alex, Jan

    IPC分类号： C12N5/10 ,  C12N15/86

    CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10352

    摘要： Cells capable of at least, in part, complementing adenovirus E2A function of an adenovirus defective in E2A function. Such cells include a nucleic acid encoding adenovirus E2A or a functional part, derivative and/or analogue thereof, integrated into the genome of the cell. Preferably, the cell has E2A nucleic acid derived from a temperature sensitive adenovirus such as adenovirus ts125. Methods for producing an adenovirus particle containing an adenovirus vector with a functional deletion of E2A. Such a method involves providing a cell as previously described with the functionally deleted adenovirus vector, culturing the cell, and harvesting the virus particle. The functional deletion can comprise a deletion of at least part of the nucleic acid encoding E2A. In such a method, the nucleic acid encoding adenovirus E2A in the genome of the cell preferably has no sequence overlap with the vector which leads to replication competent adenovirus and/or to the formation of an adenovirus vector comprising E2A function. In the method, the adenovirus vector preferably further comprises a functional deletion of E1-region encoding nucleic acid. Adenovirus vectors comprising a functional deletion of adenovirus E2A, preferably a deletion of at least part of the nucleic acid encoding E2A. Preparations of adenovirus vector containing adenovirus particles wherein the adenovirus vector comprises a functional deletion of E2A. Such an adenovirus vector preferably further includes a deletion of nucleic acid encoding E1-region proteins, and may be free of adenovirus vectors comprising E2A function. Methods for providing cells of an individual with a nucleic acid of interest, without risk of administering simultaneously a replication competent adenovirus vector, comprising administering the individual one of the previously described preparations.

    POLY(ORGANO)PHOSPHAZENES FOR USE IN SYNTHETIC TRANSFECTION SYSTEMS
    3.
    发明公开
    POLY(ORGANO)PHOSPHAZENES FOR USE IN SYNTHETIC TRANSFECTION SYSTEMS 失效
    POLY（ORGANO）PHOSPHAZENE IN SYNTHETICHEN TRANSFEKTIONS-SYSTEMEN

    公开(公告)号：EP0787201A1

    公开(公告)日：1997-08-06

    申请号：EP96926662.0

    申请日：1996-08-16

    申请人： Introgene B.V. ,  OctoPlus B.V. ,  Universiteit Utrecht

    发明人： HENNINK, Wilhelmus, Everhardus ,  BOUT, Abraham

    IPC分类号： C12N15 ,  A61K47 ,  A61K48 ,  C12N5

    CPC分类号： C12N15/87 ,  A61K47/605 ,  A61K48/00

    摘要： The present invention relates to a synthetic transfection system comprising as a carrier a cationic, water soluble or water dispersible polyphosphazene. In addition, it relates to a method for introducing DNA fragments in target cells, comprising contacting these DNA fragments with a polyphosphazene which is at least partially substituted with cationic substituents and subsequently contacting the obtained transfection system wits target cells. Finally, the invention involves the use of a polyphosphazene which is at least partially substituted with cationic substituents as transfection vehicle.

    摘要翻译： PCT No.PCT / NL96 / 00324 Sec。 371日期：1997年6月30日 102（e）日期1996年6月30日PCT提交1996年8月16日PCT公布。 出版物WO97 / 07226 日本1997年2月27日本发明涉及一种合成转染系统，其包含阳离子，水分散性聚磷腈作为载体。 此外，本发明涉及在靶细胞中引入DNA片段的方法，包括使这些DNA片段与至少部分被阳离子取代基部分取代的聚磷腈接触，随后将获得的转染系统与靶细胞接触。 最后，本发明涉及使用至少部分被阳离子取代基取代的聚磷腈作为转染载体。