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公开(公告)号：EP3831935A1
公开(公告)日：2021-06-09
申请号：EP19844983.7
申请日：2019-07-30
发明人： IMAGAWA, Kiwamu , MINAMI, Kotaro , MAEDA, Kenichi , HOSODA, Yuki , WATANABE, Shunsuke , SATO, Kazutoshi , HIGASHIGUCHI, Yasuna , KUSHIDA, Takashi , ISHIWARI, Ayumi
IPC分类号： C12N5/0775 , A61K35/32 , A61K35/545 , A61P19/02 , A61P19/04 , A61P29/00 , A61P37/06
摘要： Pluripotent stem cell-enriched dental pulp-derived cells that can be administered to humans as a medicine, and a production method thereof are disclosed. The production method is a method for producing dental pulp-derived cells enriched with pluripotent stem cells including: a step (a) of digesting dental pulp with a protease to prepare a dental pulp suspension; a step (b) of culturing the suspension to proliferate pluripotent stem cells contained in the suspension; a step (c) of freezing the proliferated pluripotent stem cells in a state in which the pluripotent stem cells are suspended in a first cryopreservation liquid; a step (d) of thawing the frozen pluripotent stem cells; a step (e) of culturing the thawed pluripotent stem cells in a state in which the pluripotent stem cells are adhered to surfaces of particles to proliferate the pluripotent stem cells on the surfaces of the particles; and a step (f) of bringing the particles into contact with a protease to separate the pluripotent stem cells adhered to the surfaces of the particles from the particles.
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2.发明公开
公开(公告)号：EP3219322A1
公开(公告)日：2017-09-20
申请号：EP15859662.7
申请日：2015-11-13
申请人： JCR Pharmaceuticals CO., LTD. , Nippon Medical School Foundation , National Center of Neurology and Psychiatry
发明人： OKADA, Takashi , KASAHARA, Yuko , IMAGAWA, Kiwamu
IPC分类号： A61K35/32 , A61P21/00 , C12N5/0775
CPC分类号： C12N5/0664 , A61K35/00 , A61K35/32 , C12N5/00 , C12N5/0607 , C12N2533/52 , C12N2533/54 , C12N2535/00
摘要： Disclosed are a therapeutic agent for muscular dystrophy employing pluripotent stem cells obtained from dental pulp and a method for preparation thereof. The therapeutic agent for muscular dystrophy comprises pluripotent stem cell-enriched human dental pulp-derived cells as the active ingredient, and is prepared by a method of preparation comprising the steps of: (a) adding dental pulp-derived cells contained in a dental pulp suspension, in a culture vessel containing feeder cells whose proliferative ability is suppressed, onto a membrane having micropores that can block feeder cells from passing therethrough and supported within the vessel in a manner that avoids direct contact of the lower side face thereof with the feeder cells, and culturing the dental pulp-derived cells on the membrane while preventing direct contact with the feeder cells, and (b) recovering the cells having grown on the membrane as the pluripotent stem cell-enriched human dental pulp-derived cells.
摘要翻译： 公开了使用从牙髓得到的多能干细胞的肌营养不良症治疗剂及其制备方法。 该肌营养不良症治疗剂包含富含多能干细胞的人类牙髓来源的细胞作为有效成分，并且通过包括以下步骤的制备方法来制备：（a）添加牙髓中含有的牙髓来源的细胞 悬浮在含有抑制增殖能力的饲养细胞的培养容器中，悬浮在具有可阻断饲养细胞的微孔的膜上，并以避免其下侧面与饲养细胞直接接触的方式支持在容器内 并且在防止与饲养细胞直接接触的同时在膜上培养牙髓源细胞，以及（b）回收已经在膜上生长的细胞作为富含多能干细胞的人牙髓来源的细胞。
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公开(公告)号：EP2210608B1
公开(公告)日：2016-08-31
申请号：EP08844376.7
申请日：2008-10-27
IPC分类号： A61K35/28 , A61K35/12 , A61K35/48 , A61K35/50 , A61K47/02 , A61K47/20 , A61K47/42 , A61L27/00
CPC分类号： A61K9/10 , A61K9/0019 , A61K35/28 , A61K35/48 , A61K2035/124 , C12N5/0663 , C12N2500/60 , C12N2501/70
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公开(公告)号：EP4098267A1
公开(公告)日：2022-12-07
申请号：EP21748145.6
申请日：2021-01-29
发明人： IMAGAWA, Kiwamu , MINAMI, Kohtaro , MAEDA, Kenichi , HOSODA, Yuki , WATANABE, Shunsuke , SATO, Kazutoshi , HIGASHIGUCHI, Yasuna , KUSHIDA, Takashi , ISHIWARI, Ayumi , TSUSHIMA, Yu , MIYAUCHI, Yoshiteru
IPC分类号： A61K35/28 , A61K9/10 , A61K47/02 , A61K47/12 , A61K47/20 , A61K47/22 , A61K47/42 , A61P1/18 , A61P7/04 , A61P9/04 , A61P9/10 , A61P9/14 , A61P11/00 , A61P11/06 , A61P17/00 , A61P27/02 , A61P29/00 , A61P31/04 , A61P31/12 , A61P31/14 , A61P31/16
摘要： [Problem] To provide a pharmaceutical composition having a novel use comprising a dental pulp-derived multipotent stem cell preparation that can be administered to humans.
[Solution] A pharmaceutical composition comprising dental pulp-derived stem cells as an active ingredient for suppressing infiltration into a tissue of at lease neutrophils, monocytes, or lymphocytes.-
5.发明公开
公开(公告)号：EP3871748A1
公开(公告)日：2021-09-01
申请号：EP19876978.8
申请日：2019-10-21
摘要： A filtration filter is configured from a first weld frame, a second weld frame, and a filter sandwiched and welded between the first weld frame and the second weld frame. The first weld frame and the second weld frame are configured by a flexible film having a film thickness of at least 120 µm. The filter is configured by a substance having a higher melting point than the first weld frame and the second weld frame and having an opening area ratio of from 10% to 80%. The first weld frame is configured by a polymer that includes either a high density polyethylene having a melting point of from 120°C to 140°C, a linear low density polyethylene having a melting point of from 105°C to 125°C, or a mixture including at least one of the high density polyethylene or the linear low density polyethylene.
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公开(公告)号：EP2832850B1
公开(公告)日：2017-09-06
申请号：EP13768121.9
申请日：2013-03-28
发明人： IMAGAWA, Kiwamu
IPC分类号： C12N5/0775 , A61K35/32 , A61P19/08 , A61P37/06 , A61P43/00
CPC分类号： C12N5/0607 , A61K35/12 , A61K35/32 , C12N5/0664 , C12N2533/52 , C12N2533/54 , C12N2535/00 , C12N2535/10
摘要： Disclosed is a method for producing pluripotent stem cell-enriched human dental pulp-derived cells. The method is characterized in that it includes (a) culturing a dental pulp suspension in a feeder cells-culture vessel containing feeder cells whose proliferative ability is suppressed, on a membrane having micropores that can block passage of the feeder cells and supported in the feeder cells-culture vessel in a manner to prevent the lower side face thereof from contacting with the feeder cells, thereby preventing direct contact with the feeder cells, and, (b) a step for recovering the cells proliferating on the membrane.
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公开(公告)号：EP2832850A1
公开(公告)日：2015-02-04
申请号：EP13768121.9
申请日：2013-03-28
发明人： IMAGAWA, Kiwamu
IPC分类号： C12N5/0775 , A61K35/32 , A61P19/08 , A61P37/06 , A61P43/00
CPC分类号： C12N5/0607 , A61K35/12 , A61K35/32 , C12N5/0664 , C12N2533/52 , C12N2533/54 , C12N2535/00 , C12N2535/10
摘要： Disclosed is a method for producing pluripotent stem cell-enriched human dental pulp-derived cells. The method is characterized in that it includes (a) culturing a dental pulp suspension in a feeder cells-culture vessel containing feeder cells whose proliferative ability is suppressed, on a membrane having micropores that can block passage of the feeder cells and supported in the feeder cells-culture vessel in a manner to prevent the lower side face thereof from contacting with the feeder cells, thereby preventing direct contact with the feeder cells, and, (b) a step for recovering the cells proliferating on the membrane.
摘要翻译： 公开了一种用于生产富含多能干细胞的人类牙髓来源的细胞的方法。 该方法的特征在于，包括：（a）将牙髓悬浮液在含有抑制增殖能力的饲养细胞的饲养细胞培养容器中培养在具有可阻塞饲养细胞通过并支持在饲养细胞中的微孔的膜上 以防止其下侧面与饲养细胞接触，从而防止与饲养细胞直接接触，和（b）回收在细胞膜上增殖的细胞的步骤。
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公开(公告)号：EP2894218B1
公开(公告)日：2018-09-12
申请号：EP13835476.6
申请日：2013-09-05
发明人： HAGIYA, Michio , IMAGAWA, Kiwamu , HOSODA, Yuuki , KOIZUMI, Noriko , OKUMURA, Naoki , NAKAHARA, Makiko , KINOSHITA, Shigeru
CPC分类号： C12N5/0621 , A61K35/30 , A61K35/44 , C12N5/0018 , C12N2500/84 , C12N2502/1358
摘要： [Problem]To provide, in order to manufacture cells to transplant into patients with corneal endothelial failure, a medium used to culture corneal endothelial cells obtained from human corneal tissue and grow said cells while maintaining the morphology thereof as corneal endothelial cells. [Solution] A medium containing a conditioned medium from mesenchymal stem cells; and a method in which said medium is used to culture corneal endothelial cells.
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公开(公告)号：EP2975117A1
公开(公告)日：2016-01-20
申请号：EP14762919.0
申请日：2014-03-09
发明人： IMAGAWA, Kiwamu , MAEDA, Kenichi , HOSODA, Yuki , YOKOYAMA, Shuichi , OKUMURA, Naoki , KOIZUMI, Noriko , KINOSHITA, Shigeru
CPC分类号： A61K35/36 , A61L27/3813 , A61L2430/16 , C12N5/0602 , C12N5/0621 , C12N2501/15 , C12N2501/727 , C12N2501/999 , C12N2502/1352
摘要： [Problem] To provide a method for producing human corneal epithelial sheet, wherein the human corneal epithelial-derived cells obtained by culturing human corneal epithelial cells are cultured on an amnion substrate.
[Solution] A method for culturing human corneal epithelial cells using mesenchymal stem cells as the feeder cells; and a method for culturing human corneal epithelial cells using a medium containing a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor and a TGF-6 receptor inhibitor in various combinations.摘要翻译： 本发明提供一种人角膜上皮片的制造方法，其中将人角膜上皮细胞培养得到的人角膜上皮来源细胞在羊膜基质上进行培养。 [解决方法]使用间充质干细胞作为饲养细胞来培养人角膜上皮细胞的方法; 以及使用含有ROCK抑制剂，磷酸二酯酶抑制剂，MAP激酶抑制剂和TGF-β受体抑制剂的介质以各种组合培养人角膜上皮细胞的方法。
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10.发明公开MEDIUM, FOR CULTURING CORNEAL ENDOTHELIAL CELLS, CONTAINING CONDITIONED MEDIUM FROM MESENCHYMAL STEM CELLS 有权中号ZURZÜCHTUNGVON HORNHAUTENDOTHELZELLEN MIT EINEM KONDITIONIERTEN MEDIUM AUS MESENCHYMALEN STAMMZELLEN
公开(公告)号：EP2894218A1
公开(公告)日：2015-07-15
申请号：EP13835476.6
申请日：2013-09-05
发明人： HAGIYA, Michio , IMAGAWA, Kiwamu , HOSODA, Yuuki , KOIZUMI, Noriko , OKUMURA, Naoki , NAKAHARA, Makiko , KINOSHITA, Shigeru
IPC分类号： C12N1/00 , A61K35/44 , A61P27/02 , C12N5/0775
CPC分类号： C12N5/0621 , A61K35/30 , A61K35/44 , C12N5/0018 , C12N2500/84 , C12N2502/1358
摘要： [Problem]To provide, in order to manufacture cells to transplant into patients with corneal endothelial failure, a medium used to culture corneal endothelial cells obtained from human corneal tissue and grow said cells while maintaining the morphology thereof as corneal endothelial cells. [Solution] A medium containing a conditioned medium from mesenchymal stem cells; and a method in which said medium is used to culture corneal endothelial cells.
摘要翻译： 为了将细胞移植到患有角膜内皮细胞衰竭的患者中，提供了用于培养从人角膜组织获得的角膜内皮细胞培养的培养基，并在将其形态保持为角膜内皮细胞的同时生长所述细胞。 [溶液]含有来自间充质干细胞的条件培养基的培养基; 以及使用所述培养基培养角膜内皮细胞的方法。
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