公开(公告)号：EP1473363A1

公开(公告)日：2004-11-03

申请号：EP03700514.7

申请日：2003-01-09

申请人： Japan Science and Technology Agency

发明人： KOKUBO, Tetsuro ,  SHIRAKAWA, Masahiro ,  TAME, J.R.H.

IPC分类号： C12N15/09 ,  C12Q1/68 ,  G01N24/08

CPC分类号： C12Q1/6809 ,  C12Q1/6897 ,  G01N24/08 ,  G01R33/465 ,  C12Q2565/633

摘要： The present invention provides a technique by which highly detailed gene expression profiling data for analyzing the gene function can be obtained safely and conveniently. It is a versatile non-destructive and high-resolution visualizing technique by which expression levels of genes can be monitored in real time, and which can be applied in vivo and to deep tissues. For instance, plasmids in which molecules that vary the NMR signal and can be quantified by PHM genes are incorporated downstream from promoters responding to the condition of medium, external stimuli and the like, are introduced into the cells. The level of polyphosphate in deep tissues and the like can then be quantified by 31 P-NMR, allowing expression profiling of the promoter, both non-destructively and in real time.

摘要翻译： 本发明提供了可以安全，方便地获得用于分析基因功能的高度详细的基因表达谱分析数据的技术。 它是一种多功能的非破坏性和高分辨率的可视化技术，可以实时监测基因的表达水平，并且可以在体内和深层组织中应用。 例如，将其中改变NMR信号并且可以由PHM基因定量的分子的质粒引入到响应于培养基，外部刺激等的条件的启动子的下游引入细胞中。 深层组织中的多磷酸盐水平等可以通过P-NMR进行定量，从而可以非破坏性地和实时地表达启动子的表达谱。

公开(公告)号：EP2907792A1

公开(公告)日：2015-08-19

申请号：EP13844813.9

申请日：2013-10-10

申请人： Japan Science and Technology Agency

发明人： SHIRAKAWA, Masahiro ,  SOTOMA, Shingo ,  IGARASHI, Ryuji ,  HARADA, Yoshie

IPC分类号： C01B31/06 ,  G01N21/64 ,  G01N24/00

CPC分类号： G01N33/6803 ,  C01B32/25 ,  C01B32/28 ,  G01N21/6428 ,  G01N24/087 ,  G01N24/10 ,  G01N33/582 ,  G01N2021/6439 ,  G01R33/323 ,  G01R33/60 ,  Y10T436/203332 ,  Y10T436/23 ,  Y10T436/24

摘要： A nanodiamond particle including an NV center having ODMR intensity enhanced, of which surface is modified with a functional group containing a heteroatom, is provided. This nanodiamond particle as being chemically modified can serve for a fluorescent molecular probe which can be made use of in a biological system. By tracking a rotational motion of the NV center included in this fluorescent molecular probe, structural change of a protein can be analyzed in real time. The functional group containing a heteroatom can be at least any functional group of a hydroxyl group and a hydroxyalkyl group, or a carboxyl group.

摘要翻译： 包括AT NV中心一种纳米金刚石颗粒具有ODMR强度增强，哪个表面用含有杂原子的官能团，提供了修改。 这种纳米金刚石颗粒为被化学修饰可以用于荧光分子探针，其可以在生物系统中进行的使用。通过跟踪NV中心的旋转运动包括在该荧光分子探针，蛋白质的结构变化可以在分析 实时。 含有杂原子的官能团可以是至少一个羟基基团的任何官能团和羟基烷基，或羧基。