公开(公告)号：EP1818412A1

公开(公告)日：2007-08-15

申请号：EP05809660.3

申请日：2005-11-28

申请人： Jimro Co., Ltd.

发明人： NAKANO, Takamitu ,  NIIMI, Manabu ,  OGIHARA, Fumiko

IPC分类号： C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  G01N33/92 ,  G01N33/48

CPC分类号： C12Q1/44 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/92

摘要： There are provided a method and reagent for measuring cholesterol in remnant-like lipoprotein in a sample with high sensitivity by more simple operation. The method for measuring cholesterol in remnant-like lipoprotein uses a cholesterol esterase, in which the activity ratio of a lipoprotein lipase to a cholesterol esterase (lipoprotein lipase activity/cholesterol esterase activity) is from 12 to 7000 in a method for measuring cholesterol in the lipoprotein by measuring hydrogen peroxide or a reduced coenzyme obtained by allowing the cholesterol esterase and a cholesterol oxidase or a cholesterol dehydrogenase to act on a test sample containing a lipoprotein.

摘要翻译： 提供了通过更简单的操作在高灵敏度下测量样品中残留样脂蛋白胆固醇的方法和试剂。 用于测定残留样脂蛋白中的胆固醇的方法使用胆固醇酯酶，其中脂蛋白脂肪酶与胆固醇酯酶的活性比（脂蛋白脂肪酶活性/胆固醇酯酶活性）为12〜7000。 通过测量过氧化氢或通过使胆固醇酯酶和胆固醇氧化酶或胆固醇脱氢酶作用于含有脂蛋白的测试样品而获得的还原型辅酶的脂蛋白。