公开(公告)号：EP1975244A1

公开(公告)日：2008-10-01

申请号：EP07706273.5

申请日：2007-01-19

申请人： Jimro Co., Ltd.

发明人： OGIHARA, Fumiko ,  NIIMI, Manabu ,  NAKANO, Takamitsu ,  KANATANI, Kazushi

IPC分类号： C12Q1/44 ,  C12Q1/26 ,  C07K16/18

CPC分类号： C07K16/18 ,  C12Q1/46 ,  G01N33/92 ,  G01N2333/775

摘要： A method for measuring cholesterol in remnant-like lipoprotein in a sample, by a more convenient operation and with high sensitivity, and a reagent for the measurement are provided.
A method for measuring cholesterol in remnant-like lipoprotein, the method comprising subjecting a test sample containing lipoproteins to the action of a cholesterol esterase and a cholesterol oxidase or cholesterol dehydrogenase, and measuring the hydrogen peroxide or reduced coenzyme generated by the enzymatic reaction, wherein a surfactant having a benzenesulfonic acid structure in the molecule or a polyacrylic acid-based polymeric surfactant is used.

摘要翻译： 提供了通过更方便的操作和高灵敏度测量样品中残留样脂蛋白胆固醇的方法和用于测量的试剂。 一种测定残留样脂蛋白中的胆固醇的方法，其特征在于，使含有脂蛋白的试验样品对胆固醇酯酶和胆固醇氧化酶或胆固醇脱氢酶的作用进行测定，并测定通过酶反应产生的过氧化氢或还原型辅酶， 使用分子中具有苯磺酸结构的表面活性剂或聚丙烯酸类聚合物表面活性剂。

公开(公告)号：EP1818412A1

公开(公告)日：2007-08-15

申请号：EP05809660.3

申请日：2005-11-28

申请人： Jimro Co., Ltd.

发明人： NAKANO, Takamitu ,  NIIMI, Manabu ,  OGIHARA, Fumiko

IPC分类号： C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  G01N33/92 ,  G01N33/48

CPC分类号： C12Q1/44 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/92

摘要： There are provided a method and reagent for measuring cholesterol in remnant-like lipoprotein in a sample with high sensitivity by more simple operation. The method for measuring cholesterol in remnant-like lipoprotein uses a cholesterol esterase, in which the activity ratio of a lipoprotein lipase to a cholesterol esterase (lipoprotein lipase activity/cholesterol esterase activity) is from 12 to 7000 in a method for measuring cholesterol in the lipoprotein by measuring hydrogen peroxide or a reduced coenzyme obtained by allowing the cholesterol esterase and a cholesterol oxidase or a cholesterol dehydrogenase to act on a test sample containing a lipoprotein.

摘要翻译： 提供了通过更简单的操作在高灵敏度下测量样品中残留样脂蛋白胆固醇的方法和试剂。 用于测定残留样脂蛋白中的胆固醇的方法使用胆固醇酯酶，其中脂蛋白脂肪酶与胆固醇酯酶的活性比（脂蛋白脂肪酶活性/胆固醇酯酶活性）为12〜7000。 通过测量过氧化氢或通过使胆固醇酯酶和胆固醇氧化酶或胆固醇脱氢酶作用于含有脂蛋白的测试样品而获得的还原型辅酶的脂蛋白。