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公开(公告)号：EP2395026A3
公开(公告)日：2011-12-21
申请号：EP11179903.7
申请日：2006-02-07
IPC分类号： C07K16/46 , C07K16/00 , C07K16/12 , C07K16/28 , A61K39/395
CPC分类号： C07K16/00 , C07K16/1271 , C07K16/2818 , C07K2317/21 , C07K2317/24 , C07K2317/567 , C07K2317/94
摘要： The present invention provides for a method for improving properties of an antibody such as an expression level and stability. A method for obtaining an antibody with an improved expression level and/or stability by modifying a human antibody or a humanized antibody, characterized by that at least any one of the amino acid residues at position 8, 12, 15 or 18 (according to Kabat numbering) in a light chain variable region (hereinafter referred to as "VL chain") of a human antibody or a humanized antibody is substituted with a different amino acid other than proline or cysteine, and a human antibody or a humanized antibody or a human antibody fragment or a humanized antibody fragment with an improved expression level and/or stability which are obtained by said method.
摘要翻译： 本发明提供了用于改善抗体性质如表达水平和稳定性的方法。 一种通过修饰人抗体或人源化抗体获得具有改进的表达水平和/或稳定性的抗体的方法，其特征在于至少8,12,15或18位氨基酸残基中的任何一个（根据Kabat 编号）在人抗体或人源化抗体的轻链可变区（以下称为“VL链”）中被脯氨酸或半胱氨酸以外的其他氨基酸，人抗体或人源化抗体或人 抗体片段或人源化抗体片段，其具有通过所述方法获得的改善的表达水平和/或稳定性。
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公开(公告)号：EP2395026A2
公开(公告)日：2011-12-14
申请号：EP11179903.7
申请日：2006-02-07
IPC分类号： C07K16/46 , C07K16/00 , C07K16/12 , C07K16/28 , A61K39/395
CPC分类号： C07K16/00 , C07K16/1271 , C07K16/2818 , C07K2317/21 , C07K2317/24 , C07K2317/567 , C07K2317/94
摘要： The present invention provides for a method for improving properties of an antibody such as an expression level and stability. A method for obtaining an antibody with an improved expression level and/or stability by modifying a human antibody or a humanized antibody, characterized by that at least any one of the amino acid residues at position 8, 12, 15 or 18 (according to Kabat numbering) in a light chain variable region (hereinafter referred to as "VL chain") of a human antibody or a humanized antibody is substituted with a different amino acid other than proline or cysteine, and a human antibody or a humanized antibody or a human antibody fragment or a humanized antibody fragment with an improved expression level and/or stability which are obtained by said method.
摘要翻译： 本发明提供了改善抗体性质的方法，例如表达水平和稳定性。 通过修饰人抗体或人源化抗体获得具有改善的表达水平和/或稳定性的抗体的方法，其特征在于，第8,12,15或18位的氨基酸残基中的至少任一种（根据Kabat 编号）在人抗体或人源化抗体的轻链可变区（以下称为“VL链”）中被除脯氨酸或半胱氨酸以外的不同氨基酸，人抗体或人源化抗体或人 抗体片段或具有通过所述方法获得的改善的表达水平和/或稳定性的人源化抗体片段。
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公开(公告)号：EP0212522A3
公开(公告)日：1987-07-15
申请号：EP86111064
申请日：1986-08-11
IPC分类号： G01N33/74 , G01N33/577 , G01N33/543
摘要： Described is an improved method for the measurement of TSH in blood or body liquid by immunoassays such as EIA and RIA, characterized in that TSH-P subunit specific monoclonal antibodies recognizing different epitopes are used. According to this method, TSH can easily be measured in high sensitivity with high specificity within a very short period of time without inhibition by the presence of other glycoprotein hormones such as LH, HCG and FSH. This method is useful for diagnosis of various diseases in which TSH plays a role, such as proto- phathic hypothyroidism, Basedow's disease and cretinism.
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4.发明公开Method for the determination of thyroid-stimulating hormone 失效Verfahren zur Bestimmung des thyroid-stimulierenden Hormons。
公开(公告)号：EP0173973A2
公开(公告)日：1986-03-12
申请号：EP85110915.7
申请日：1985-08-29
IPC分类号： G01N33/577 , G01N33/74 , C12P21/00
CPC分类号： G01N33/74
摘要： Described is a highly sensitive immunoassay method for determining the amounts of TSH contained in body fluids, in which an anti-β-subunit TSH monoclonal antibody having a specific association constant value is used.
摘要翻译： 描述了用于确定体液中含有的TSH的量的高度灵敏的免疫测定方法，其中使用具有特定关联常数值的抗β-亚基TSH单克隆抗体。
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公开(公告)号：EP0173341A1
公开(公告)日：1986-03-05
申请号：EP85110914.0
申请日：1985-08-29
IPC分类号： G01N33/74 , G01N33/76 , G01N33/78 , G01N33/577
CPC分类号： C07K16/26
摘要： Described is an immunoassay method which makes it possible to determine the amounts of thyroid-stimulating hormone, follicle-stimulating hormone, luteinizing hormone and human choriogonadotropin with the substantially same degrees of sensitivities for all the hormones, in which there is used a monoclonal antibody derived from a hybridoma obtained using the spleen cells of an animal immunized with only one of the hormones or its a-subunit.
摘要翻译： 描述了一种免疫测定方法，其可以确定促甲状腺激素，促卵泡激素，黄体生成激素和人绒毛膜促性腺激素的量，其对于所有激素具有基本相同程度的敏感性，其中使用衍生的单克隆抗体 来自使用仅用一种激素或其α亚基免疫的动物的脾细胞获得的杂交瘤。
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6.发明公开HUMAN ANTIHUMAN INTERLEUKIN-6 ANTIBODY AND FRAGMENT OF THE ANTIBODY 审中-公开MENSCHLICHER ANTI-MENSCH-INTERLEUKIN-6-ANTIKÖRPERSOWIE FRAGMENT DESANTIKÖRPERS
公开(公告)号：EP1536012A1
公开(公告)日：2005-06-01
申请号：EP03791369.6
申请日：2003-08-28
IPC分类号： C12N15/13 , C07K16/24 , A61K39/395 , A61P19/02 , A61P29/00
CPC分类号： C07K16/248 , A61K2039/505 , C07K16/005 , C07K2317/21 , C07K2317/622 , C07K2317/73
摘要： A substance effective for treating immunopathy where interleukin 6 (IL-6) is involved is provided. A human anti-human IL-6 antibody and a human anti-human IL-6 antibody fragment having a high affinity to human IL-6 were obtained using phage antibody technique. Said antibody and antibody fragment are expected to be useful as a medicament for treating inflammation and immunopathy caused by IL-6.
摘要翻译： 提供了有效治疗涉及白细胞介素6（IL-6）的免疫病的物质。 使用噬菌体抗体技术获得对人IL-6具有高亲和力的人抗人IL-6抗体和人抗人IL-6抗体片段。 预期所述抗体和抗体片段可用作治疗由IL-6引起的炎症和免疫疾病的药物。
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公开(公告)号：EP0173341B1
公开(公告)日：1990-04-04
申请号：EP85110914.0
申请日：1985-08-29
IPC分类号： G01N33/74 , G01N33/76 , G01N33/78 , G01N33/577
CPC分类号： C07K16/26
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公开(公告)号：EP0173973A3
公开(公告)日：1988-08-10
申请号：EP85110915
申请日：1985-08-29
IPC分类号： G01N33/577 , G01N33/74 , C12P21/00 , C12R01/91
CPC分类号： G01N33/74
摘要： Described is a highly sensitive immunoassay method for determining the amounts of TSH contained in body fluids, in which an anti-β-subunit TSH monoclonal antibody having a specific association constant value is used.
摘要翻译： 描述了用于确定体液中含有的TSH的量的高度灵敏的免疫测定方法，其中使用具有特定关联常数值的抗β-亚基TSH单克隆抗体。
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公开(公告)号：EP0212522A2
公开(公告)日：1987-03-04
申请号：EP86111064.1
申请日：1986-08-11
IPC分类号： G01N33/74
摘要： Described is an improved method for the measurement of TSH in blood or body liquid by immunoassays such as EIA and RIA, characterized in that TSH-P subunit specific monoclonal antibodies recognizing different epitopes are used. According to this method, TSH can easily be measured in high sensitivity with high specificity within a very short period of time without inhibition by the presence of other glycoprotein hormones such as LH, HCG and FSH. This method is useful for diagnosis of various diseases in which TSH plays a role, such as proto- phathic hypothyroidism, Basedow's disease and cretinism.
摘要翻译： 描述了通过免疫测定如EIA和RIA测量血液或体液中的TSH的改进方法，其特征在于使用识别不同表位的TSH-β亚基特异性单克隆抗体。 根据该方法，可以在非常短的时间内容易地以高特异性的高灵敏度测定TSH，而不会抑制其它糖蛋白激素如LH，HCG和FSH的抑制。 这种方法可用于诊断TSH发挥作用的各种疾病，如原发性甲状腺功能减退症，基诺病和克氏病。
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