公开(公告)号：EP1939301A1

公开(公告)日：2008-07-02

申请号：EP06810702.8

申请日：2006-09-28

申请人： KYOWA HAKKO KOGYO CO., LTD.

发明人： HARA, Kiyotaka ,  KATO, Makiko ,  MORI, Hideo

IPC分类号： C12P1/04 ,  C12N15/09

CPC分类号： C12N1/00 ,  C12P7/00 ,  C12P13/04 ,  C12P19/34 ,  C12P21/00

摘要： The present invention provides processes of producing useful substances, which include the steps of culturing in a medium microorganisms that lack from their chromosomal DNA all or part of a gene encoding a protein having the amino acid sequence shown in SEQ ID NO: 1 or a gene encoding a protein that is 80% or more homologous to the amino acid sequence shown in SEQ ID NO: 1 so as to produce and accumulate the useful substances in the culture, and recovering the useful substances from the culture.

摘要翻译： 本发明提供生产有用物质的方法，其包括以下步骤：在培养基中缺乏染色体DNA的微生物全部或部分编码具有SEQ ID NO：1所示氨基酸序列的蛋白质的基因或基因 编码与SEQ ID NO：1所示的氨基酸序列同源的80％以上的蛋白质，以在培养物中产生和积累有用物质，并从培养物中回收有用物质。

公开(公告)号：EP1829965A1

公开(公告)日：2007-09-05

申请号：EP05809612.4

申请日：2005-11-25

申请人： KYOWA HAKKO KOGYO CO., LTD.

发明人： MIZOGUCHI, Hiroshi ,  HARA, Kiyotaka ,  MORI, Hideo

IPC分类号： C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12P7/64 ,  C12P13/00 ,  C12P13/04 ,  C12P17/00 ,  C12P19/00 ,  C12P19/30 ,  C12P19/38 ,  C12P21/02

CPC分类号： C12P13/08 ,  C12N1/20 ,  C12R1/185

摘要： According to the present invention, the E . coli mutant strain having chromosomal DNA that is at least 470 kbp shorter than that of a wild-type E . coli strain exhibits such a property that the number of the cells after a certain period of culture is greater than a wild-type strain. Thus, by culturing the mutant strain in a medium so as to generate and accumulate a useful substance in the culture, a useful substance such as proteins, peptides, amino acids, nucleic acids, vitamins, saccharides, organic acids, and lipids can be efficiently produced.

摘要翻译： 根据本发明，具有比野生型大肠杆菌菌株短至少470kbp的染色体DNA的大肠杆菌突变株显示出这样的性质，即培养一段时间后的细胞数目是 大于野生型菌株。 因此，通过在培养基中培养突变菌株以在培养物中产生并积累有用物质，可以有效地使蛋白质，肽，氨基酸，核酸，维生素，糖类，有机酸和脂质等有用物质 产生的。