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公开(公告)号：EP0185092B1
公开(公告)日：1992-10-07
申请号：EP85901555.4
申请日：1985-03-12
发明人： FUJIO, Tatsuro , MARUYAMA, Akihiko , NISHI, Tatsunari , OZAKI, Akio , ITO, Seiga , OZAKI, Atsuko
摘要： GMP can be prepared in a good yield by converting XMP, ammonia and/or L-glutamine in an aqueous solution using a culture product, cells or their treated product of E. coli having GMP synthetase activity and the ability of converting AMP to ATP in the presence of an energy source other than phosphorus oxides, in the presence of the energy source. GMP can also be prepared in a good yield by converting XMP, ammonia and/or L-glutamine to GMP in an aqueous medium in the presence of ATP using a culture product, cells or their treated product of transformant obtained by using a recombinant DNA of a DNA fragment containing GMP synthetase gene and a vector DNA fragment. The process of using transformant having ATP-reproducing ability is extremely advantageous.
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公开(公告)号：EP0185092A1
公开(公告)日：1986-06-25
申请号：EP85901555.4
申请日：1985-03-12
发明人： FUJIO, Tatsuro , MARUYAMA, Akihiko , NISHI, Tatsunari , OZAKI, Akio , ITO, Seiga , OZAKI, Atsuko
摘要： GMP can be prepared in a good yield by converting XMP, ammonia and/or L-glutamine in an aqueous solution using a culture product, cells or their treated product of E. coli having GMP synthetase activity and the ability of converting AMP to ATP in the presence of an energy source other than phosphorus oxides, in the presence of the energy source. GMP can also be prepared in a good yield by converting XMP, ammonia and/or L-glutamine to GMP in an aqueous medium in the presence of ATP using a culture product, cells or their treated product of transformant obtained by using a recombinant DNA of a DNA fragment containing GMP synthetase gene and a vector DNA fragment. The process of using transformant having ATP- reproducing ability is extremely advantageous.
摘要翻译： 通过使用培养产物，细胞或其具有GMP合成酶活性的大肠杆菌处理产物以及将AMP转化为ATP的能力，在水溶液中转化XMP，氨和/或L-谷氨酰胺，可以以良好的产率制备GMP 在能源存在的情况下存在除磷氧化物之外的能源。 通过使用培养产物，细胞或它们的转化体处理产物，在ATP存在下，在水性介质中将XMP，氨和/或L-谷氨酰胺转化为GMP，也可以以良好的产率制备GMP，所述转化体通过使用 含有GMP合成酶基因的DNA片段和载体DNA片段。 使用具有ATP再生能力的转化体的过程是非常有利的。
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