公开(公告)号：EP2455484B1

公开(公告)日：2018-12-05

申请号：EP10800069.6

申请日：2010-07-19

申请人： Korea Advanced Institute of Science and Technology

发明人： LEE, Sang Yup ,  CHOI, Yong Jun

IPC分类号： C12P7/64 ,  C12N1/21 ,  C12N15/55

CPC分类号： C12N9/20 ,  C12P7/649 ,  C12Y301/01003 ,  Y02E50/13 ,  Y02P20/52

摘要： The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel.

公开(公告)号：EP2455484A2

公开(公告)日：2012-05-23

申请号：EP10800069.6

申请日：2010-07-19

申请人： Korea Advanced Institute of Science and Technology

发明人： LEE, Sang Yup ,  CHOI, Yong Jun

IPC分类号： C12P7/64 ,  C12N1/21 ,  C12N15/55

CPC分类号： C12N9/20 ,  C12P7/649 ,  C12Y301/01003 ,  Y02E50/13 ,  Y02P20/52

摘要： The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel.

摘要翻译： 本发明涉及使用微生物具有产生油生产的脂肪酸烷基酯的方法的能力，更具体地，该方法包括具有产生油的能力培养微生物产生的脂肪酸烷基酯的方法，从而 累积大量的油在微生物，诱导所述微生物产生的油的自溶，以产生游离脂肪酸，游离脂肪酸转化成在烷基酯。 。根据本发明的方法中，油积累在微生物中，：如三酰甘油确实是由微生物产生的典型的油，可以被转换成使用代谢工程方法以高效率的脂肪酸烷基酯。 因此，本发明的方法是用于工业生产最近已经发现是有效的作为生物柴油的脂肪酸烷基酯是有用的。

公开(公告)号：EP2592138B1

公开(公告)日：2018-08-08

申请号：EP11792710.3

申请日：2011-06-10

申请人： Korea Advanced Institute Of Science And Technology

发明人： LEE, Sang Yup ,  CHOI, Yong Jun

IPC分类号： C12N1/21 ,  C12N15/52 ,  C12P5/00

CPC分类号： C12P5/00 ,  C07K14/245 ,  C12N9/001 ,  C12N9/18 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70 ,  C12P5/005 ,  C12P5/02 ,  C12P5/026

摘要： The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.

公开(公告)号：EP2592138A2

公开(公告)日：2013-05-15

申请号：EP11792710.3

申请日：2011-06-10

申请人： Korea Advanced Institute Of Science And Technology

发明人： LEE, Sang Yup ,  CHOI, Yong Jun

IPC分类号： C12N1/21 ,  C12N15/52 ,  C12P5/00

CPC分类号： C12P5/00 ,  C07K14/245 ,  C12N9/001 ,  C12N9/18 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70 ,  C12P5/005 ,  C12P5/02 ,  C12P5/026

摘要： The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.

摘要翻译： 本发明涉及到的微生物的变体具有生产烃类，包括烷烃的能力，并且生产烃，包括烷烃，使用相同的，更具体的方法中，通过引入基因编码的酶转换脂肪酸的得到的微生物的变体 酰基-ACP向游离脂肪酸，编码酶的游离脂肪酸转化成脂肪酰辅酶A，编码酶转化脂肪酰辅酶A的一个基因，以脂肪醛和编码酶的基因转化脂肪醛为烷烃到微生物的基因 改进以使其适用于生产烃，包括烷烃和生产烃，包括烷烃，使用相同的方法。 本发明的微生物变体具有高电势，以用于通过额外的代谢通量工程改良的菌株，并因此是用于工业生产的烃，包括烷烃是有用的。