公开(公告)号：EP2097743A1

公开(公告)日：2009-09-09

申请号：EP07851674.7

申请日：2007-12-21

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  JEONG, Hawoong ,  KIM, Tae Yong ,  KIM, Pan-Jun ,  LEE, Kwang Ho

IPC: G01N33/48

CPC classification number: C12Q1/18 ,  G01N33/5038

Abstract: The present invention disclosed is a method for screening metabolites essential for the growth of microorganism using metabolic flux analysis. More specifically, the present invention relates to the method for screening metabolites essential for the growth of microorganism, by selecting a target microorganism, constructing a metabolic network model of the selected microorganism, inactivating the consumption reaction of each of metabolites in the constructed metabolic network model, analyzing the metabolic flux of the metabolites to select metabolites essential for the growth of the microorganism, and confirming the selected metabolites using the utilization of each of the metabolites, defined as flux sum (Φ ). According to the present invention, metabolites essential for the growth of microorganism, and genes involved in the essential metabolites, can be screened in a convenient manner, and drug-target genes against pathogenic microorganisms can be predicted by deleting genes associated with the metabolites screened according to the method.

公开(公告)号：EP1915459A1

公开(公告)日：2008-04-30

申请号：EP05804474.4

申请日：2005-10-14

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  KIM, Tae Yong ,  LEE, Dong Yup

IPC: C12Q1/68

CPC classification number: G06F19/12 ,  G06F19/18

Abstract: The present invention relates to an in silico method for improving an organism on the basis of the flux sum (Φ) of metabolites, and more particularly to a method for screening key metabolites that increase the production yield of a useful substance, the method comprising defining the metabolite utilization of an organism for producing a useful substance as flux sum and perturbing the flux sum, as well as a method for improving an organism producing a useful substance, the method comprising deleting and/or amplifying genes associated with the aforementioned screened key metabolites. According to the present invention, the correlation between specific metabolites and useful substance production can be exactly predicted, so that it is possible to develop an organism having increased useful substance production by introducing and/or amplifying and/or deleting genes expressing enzymes associated with the specific metabolites. In addition, it is also possible to increase the production of a useful substance by adding specific metabolites during culture.

公开(公告)号：EP2038403A1

公开(公告)日：2009-03-25

申请号：EP07715659.4

申请日：2007-03-14

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  PARK, Jin Hwan ,  LEE, Kwang Ho ,  KIM, Tae Yong

IPC: C12N1/16

CPC classification number: C12P13/08 ,  C12N9/1014 ,  C12Y201/02011

Abstract: The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.

Abstract translation: 本发明涉及具有提高的支链氨基酸生产率的突变微生物以及使用该突变微生物生产支链氨基酸的方法。 更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码参与L-亮氨酸的酶的基因和编码L-异亮氨酸生物合成酶的基因而产生的L-缬氨酸的生产率提高的突变微生物 参与D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，从而增加其表达，以及使用该突变体微生物生产L-缬氨酸的方法。 通过位点特异性诱变和代谢途径工程产生的本发明的突变微生物可高效生产支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。