公开(公告)号：EP2759591A2

公开(公告)日：2014-07-30

申请号：EP12833157.6

申请日：2012-09-18

申请人： Korea Research Institute of Standards and Science

发明人： KANG, Duk Jin ,  PARK, Sang Ryoul ,  KIM, Sook-Kyung

IPC分类号： C12M1/40 ,  C07K1/20 ,  C12N9/14

CPC分类号： C12Q1/37 ,  C07K1/20 ,  C07K1/34 ,  C07K1/36 ,  C12M21/18 ,  C12M25/12 ,  C12P21/06

摘要： Provided are an enzyme treatment apparatus for proteins using a hollow fiber membrane and an on-line proteomics method using same. The enzyme treatment apparatus for proteins according to the present invention can increase a recovery rate of peptides, which are recovered through an enzyme treatment process, by basically solving the problem of low reproducibility of an enzyme activity which may occur during a conventional enzyme treatment process, and can also reduce a time for purification and provide higher yield by performing separation and purification through a single step. In particular, the present invention can be usefully applied for developing a disease-specific protein biomarker through a statistical analysis method having highly efficient detectability for proteins in research for finding biomarkers related to human diseases.

公开(公告)号：EP3059593A1

公开(公告)日：2016-08-24

申请号：EP14854826.6

申请日：2014-09-18

申请人： Korea Research Institute of Standards and Science

发明人： KANG, Dukjin ,  KIM, Sook-Kyung ,  LEE, Sun Young

IPC分类号： G01N33/68 ,  G01N33/53 ,  G01N27/62 ,  G01N30/72

CPC分类号： G01N33/6848 ,  G01N2333/58 ,  G01N2800/32

摘要： The present invention relates to a quantitative analysis method on the basis of an isotope-dilution mass spectrometry using a chemical isotope labelling method for a brain natriuretic peptide which is widely used as a biomarker for diagnosis and prognostic evaluation of cardiovascular-associated diseases. The quantitative analysis method of a brain natriuretic peptide in blood using a chemical isotope labelling technique is a brain natriuretic peptide-specific quantitative analysis technique which is more accurate than a conventional immunoassay using an antibody.

摘要翻译： 本发明涉及一种基于同位素稀释质谱法的定量分析方法，该方法使用广泛用作心血管相关疾病诊断和预后评估生物标志物的脑钠素肽的化学同位素标记方法。 使用化学同位素标记技术的脑利钠肽在血液中的定量分析方法是一种脑利钠肽特异性定量分析技术，其比使用抗体的常规免疫测定更准确。