公开(公告)号：EP0954563A1

公开(公告)日：1999-11-10

申请号：EP97910043.0

申请日：1997-10-10

申请人： Life Technologies, Inc. ,  Price, Paul J. ,  Gorfien, Stephen F. ,  Danner, Douglas

发明人： PRICE, Paul, J. ,  GORFIEN, Steve ,  DANNER, Douglas

IPC分类号： C12N5 ,  A61K36

CPC分类号： C12N5/0043 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/74

摘要： The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of plant derivation, such as at least one plant peptide and/or at least one plant lipid and/or at least one plant fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production.

公开(公告)号：EP0986635A1

公开(公告)日：2000-03-22

申请号：EP98901221.6

申请日：1998-01-09

申请人： Life Technologies, Inc.

发明人： PRICE, Paul, J. ,  GOLDSBOROUGH, Mindy, D. ,  TILKINS, Mary, Lynn

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12N5/08 ,  C12N5/10 ,  C12N15/00 ,  C12N1/38

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  C12N5/0043 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/163 ,  C12N2500/10 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/84 ,  C12N2500/90

摘要： The present invention provides a serum-free supplement which supports the growth of embryonic stem cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and isolating embryonic stem cells, methods for producing a transgenic animal, and methods for expressing recombinant protein in embryonic stem cells and transgenic animals.

公开(公告)号：EP0953041A1

公开(公告)日：1999-11-03

申请号：EP97941382.0

申请日：1997-09-02

申请人： LIFE TECHNOLOGIES, INC.

发明人： GORFIEN, Stephen, F. ,  FIKE, Richard, M. ,  DZIMIAN, Joyce, L. ,  GODWIN, Glenn, P. ,  PRICE, Paul, J. ,  EPSTEIN, David, A. ,  GRUBER, Dale ,  McCLURE, Don

IPC分类号： C12P21 ,  C07K14 ,  C12N5 ,  C12N7 ,  C12N9 ,  C12N15

CPC分类号： C12N5/0682 ,  C07K14/61 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0603 ,  C12N7/00 ,  C12N9/2402 ,  C12N9/2471 ,  C12N15/85 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2710/10051 ,  C12Y302/01023

摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro, using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.