公开(公告)号：EP3336174A1

公开(公告)日：2018-06-20

申请号：EP18152330.9

申请日：2007-06-04

申请人： Sackstein, Robert

发明人： Sackstein, Robert

IPC分类号： C12N5/00 ,  C12P1/00 ,  C12N5/0797 ,  C12N5/0775 ,  C12N5/0735 ,  A61K35/28 ,  A61K35/30 ,  C12N9/10

CPC分类号： C12N9/1051 ,  A61K35/28 ,  A61K35/30 ,  C12N5/0006 ,  C12N5/0623 ,  C12N5/0663 ,  C12N2500/10 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/34 ,  C12N2501/58 ,  C12N2501/59 ,  C12N2501/70 ,  C12N2506/1369

摘要： Methods and compositions for modifying glycans (e.g., glycans expressed on the surface of live cells or cell particles) are provided herein.

公开(公告)号：EP3083933A1

公开(公告)日：2016-10-26

申请号：EP14827357.6

申请日：2014-12-19

申请人： Biogen MA Inc.

发明人： YANG, William C. ,  LU, Jiuyi ,  HUANG, Yao-Ming ,  YUAN, Hang ,  KSHIRSAGAR, Rashmi ,  RYLL, Thomas

IPC分类号： C12M1/34

CPC分类号： C12N5/0018 ,  C07K16/00 ,  C07K2317/14 ,  C12N2500/10 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2511/00

摘要： Methods of improving the efficiency of production of a protein product of interest in mammalian cell culture are presented. In particular, the methods result in an increase in the quantity of a protein product produced, or decreases protein product production time in a manufacturing-scale bioreactor cell culture. The disclosed methods comprise: (a) culturing the N-1 bioreactor culture to high viable cell densities; and (b) seeding the production bioreactor culture at high viable cell seeding densities.

摘要翻译： 提高了生产的哺乳动物细胞培养目标蛋白产品的效率的方法介绍。 特别地，该方法导致在产生的蛋白质产物的量的增加，或减少蛋白质产物的生产时间在制造规模的生物反应器中细胞培养物。 盘游离缺失的方法包括：（a）培养的N-1生物反应器培养至高细胞密度可行的; 和（b）在高活细胞接种密度接种生产生物反应器培养。

公开(公告)号：EP2861717A1

公开(公告)日：2015-04-22

申请号：EP13723676.6

申请日：2013-05-17

申请人： Merck Patent GmbH

发明人： VON HAGEN, Joerg

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0018 ,  A61K9/141 ,  A61K9/19 ,  B02C23/00 ,  C12N2500/05 ,  C12N2500/10

摘要： The present invention relates to an a process for manufacturing dry powder cell culture media. The preparation and usage of mixed particles generated by co-lyophilisation leads to homogenously blended cell culture media.

公开(公告)号：EP2198016A4

公开(公告)日：2011-04-20

申请号：EP08800266

申请日：2008-09-04

申请人： CA MINISTER HEALTH & WELFARE

发明人： BELLO ALEXANDER ,  GRAY MICHAEL ,  FELDMANN HEINZ ,  KOBINGER GARY

IPC分类号： C12N7/00 ,  A61K35/76 ,  A61K39/00 ,  A61K39/23 ,  A61K48/00 ,  C07K14/015 ,  C12N7/01 ,  C12N15/35 ,  C12N15/864

CPC分类号： C12N7/00 ,  A61K48/0091 ,  C07K14/005 ,  C12N15/86 ,  C12N2500/10 ,  C12N2750/14121 ,  C12N2750/14122 ,  C12N2750/14143

公开(公告)号：EP1689857A1

公开(公告)日：2006-08-16

申请号：EP04812485.3

申请日：2004-12-01

申请人： VERTEX PHARMACEUTICALS INCORPORATED

发明人： BYRN, Randal

IPC分类号： C12N5/08 ,  C12N7/00 ,  C12Q1/70 ,  A01N1/02

CPC分类号： A01N1/02 ,  A01N1/0221 ,  C12N5/067 ,  C12N2500/10 ,  C12N2500/14 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/18 ,  C12N2501/335 ,  C12N2501/70 ,  C12N2502/13

摘要： The present invention provides compositions comprising cells that can effectively produce HCV after HCV infection, compositions for culturing the cells, methods for making the composition and methods for infecting the cells in the composition with HCV. The present invention also provides methods for assaying HCV production and methods for evaluating compounds that affect the production of HCV.

公开(公告)号：EP0986635A1

公开(公告)日：2000-03-22

申请号：EP98901221.6

申请日：1998-01-09

申请人： Life Technologies, Inc.

发明人： PRICE, Paul, J. ,  GOLDSBOROUGH, Mindy, D. ,  TILKINS, Mary, Lynn

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12N5/08 ,  C12N5/10 ,  C12N15/00 ,  C12N1/38

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  C12N5/0043 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/163 ,  C12N2500/10 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/84 ,  C12N2500/90

摘要： The present invention provides a serum-free supplement which supports the growth of embryonic stem cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and isolating embryonic stem cells, methods for producing a transgenic animal, and methods for expressing recombinant protein in embryonic stem cells and transgenic animals.

公开(公告)号：EP2729167B1

公开(公告)日：2018-03-14

申请号：EP12740235.2

申请日：2012-07-05

申请人： De Staat der Nederlanden, vert. door de Minister van VWS

发明人： VAN DE WATERBEEMD, Bas ,  VAN DER POL, Leonardus, Aldolfus

IPC分类号： A61K39/095 ,  A61P39/04

CPC分类号： A61K39/095 ,  A61K35/74 ,  A61K2039/522 ,  A61K2039/523 ,  A61K2039/55505 ,  A61K2039/70 ,  C12N1/20 ,  C12N2500/10 ,  C12N2500/60

摘要： The present invention relates to the fields of medical microbiology and vaccines. In particular the invention relates to a process for detergent-free preparation of outer membrane vesicles (OMV) of Gram negative bacteria for use in vaccines, to OMV obtainable by said process, and to a pharmaceutical composition comprising such OMV. The present invention further relates to the use of OMV of the present invention as a medicament in particular for use in a method for eliciting an immune response.

公开(公告)号：EP3070161A1

公开(公告)日：2016-09-21

申请号：EP16160469.9

申请日：2016-03-15

申请人： Stembios Technologies, Inc.

发明人： Wang, James

IPC分类号： C12N5/00 ,  C12N5/0789 ,  C12N5/0775

CPC分类号： A61K35/545 ,  A61K35/28 ,  A61K35/30 ,  A61K2035/124 ,  C12N5/0087 ,  C12N5/0647 ,  C12N5/0665 ,  C12N2500/10

摘要： A method of preparing a stem-cell containing solution includes: (1) storing a mixture of a blood sample and a divalent cation chelating agent at a temperature between 2 and 12 degrees Celsius for a period of from 3 hours to 72 hours so as to have the mixture with two or more separate layers, wherein a liquid in one of the separate layers contains platelets and stem cells; (2) collecting the liquid into a container; (3) centrifuging the liquid in the container until multiple particles suspended in the liquid are deposited at a bottom of the container, wherein the particles contain the stem cells and the platelets; and (4) re-suspending the particles in a buffer or liquid.

摘要翻译： 制备含有干细胞的溶液的方法包括：（1）在2〜12摄氏度的温度下将血液样品和二价阳离子螯合剂的混合物储存3小时至72小时，以便 具有两个或更多个单独层的混合物，其中一个分离层中的液体含有血小板和干细胞; （2）将液体收集到容器中; （3）将容器中的液体离心，直到悬浮在液体中的多个颗粒沉积在容器的底部，其中颗粒含有干细胞和血小板; 和（4）将颗粒重新悬浮在缓冲液或液体中。

公开(公告)号：EP2702143A4

公开(公告)日：2014-12-17

申请号：EP12776121

申请日：2012-04-27

申请人： BIOCON RES LTD

发明人： SRIVASTAVA RUCHIKA ,  HEMDEV SNEHA LAKSHMANDAS ,  BHATNAGAR ANKUR ,  DESAN SARAVANAN ,  GOEL ANUJ ,  IYER HARISH ,  RAJA VANA ,  RAO LAVANYA

IPC分类号： C12N5/02 ,  C07K16/28 ,  C12N1/38 ,  C12N5/00 ,  C12P21/00 ,  C12P21/08

CPC分类号： C12P21/00 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/2896 ,  C12N5/0018 ,  C12N2500/10

摘要： The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.

公开(公告)号：EP2729167A1

公开(公告)日：2014-05-14

申请号：EP12740235.2

申请日：2012-07-05

申请人： De Staat der Nederlanden, vert. door de Minister van VWS

发明人： VAN DE WATERBEEMD, Bas ,  VAN DER POL, Leonardus, Aldolfus

IPC分类号： A61K39/095 ,  A61P39/04

CPC分类号： A61K39/095 ,  A61K35/74 ,  A61K2039/522 ,  A61K2039/523 ,  A61K2039/55505 ,  A61K2039/70 ,  C12N1/20 ,  C12N2500/10 ,  C12N2500/60

摘要： The present invention relates to the fields of medical microbiology and vaccines. In particular the invention relates to a process for detergent-free preparation of outer membrane vesicles (OMV) of Gram negative bacteria for use in vaccines, to OMV obtainable by said process, and to a pharmaceutical composition comprising such OMV. The present invention further relates to the use of OMV of the present invention as a medicament in particular for use in a method for eliciting an immune response.

摘要翻译： 本发明涉及医学微生物学和疫苗领域。 特别地，本发明涉及一种无洗涤剂制备用于疫苗的革兰氏阴性细菌的外膜囊泡（OMV），可通过所述方法获得的OMV以及包含这种OMV的药物组合物的方法。 本发明还涉及本发明的OMV作为特别用于引发免疫应答的方法中的药物的用途。