公开(公告)号：EP2307577A2

公开(公告)日：2011-04-13

申请号：EP09798251.6

申请日：2009-06-25

申请人： Life Technologies Corporation

发明人： ROTHBERG, Jonathan, M. ,  HINZ, Wolfgang ,  JOHNSON, Kim, L. ,  BUSTILLO, James, M. ,  LEAMON, John, H. ,  SCHULTZ, Jonathan

IPC分类号： C12Q1/68 ,  G01N27/414

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

摘要翻译： 一种装置可以包括半导体芯片和流体组件。 半导体芯片具有井阵列和传感器阵列，传感器阵列的每个传感器与孔阵列的阱流体连通。 流体组件位于半导体芯片的顶部，并且被配置为将流体输送到半导体芯片。 流体组件包括被配置为引入流体的流动膨胀室，被配置为排出流体的出口部分和流动室部分。 流动室部分被配置为允许流体从流动膨胀室流出到出口部分，并且允许流体沿着与井阵列中的材料一起相互作用。 流动膨胀室在顶部或底部具有弯曲的壁，使得中心处的流动膨胀室的高度小于限制流体向左和向右的壁。

公开(公告)号：EP2307577B1

公开(公告)日：2015-06-03

申请号：EP09798251.6

申请日：2009-06-25

申请人： Life Technologies Corporation

发明人： ROTHBERG, Jonathan, M. ,  HINZ, Wolfgang ,  JOHNSON, Kim, L. ,  BUSTILLO, James, M. ,  LEAMON, John, H. ,  SCHULTZ, Jonathan

IPC分类号： C12Q1/68 ,  G01N27/414

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/0851 ,  B01L2400/086 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N27/4148 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2565/629

公开(公告)号：EP2342552A1

公开(公告)日：2011-07-13

申请号：EP09822323.3

申请日：2009-10-22

申请人： Life Technologies Corporation

发明人： ROTHBERG, Jonathan, M. ,  SCHULTZ, Jonathan, C. ,  JOHNSON, Kim, L. ,  REARICK, Todd, M. ,  MILGREW, Mark, James ,  MARRAN, David ,  BUSTILLO, James, M.

IPC分类号： G01N27/416 ,  C12M1/34

CPC分类号： G01N27/4145

摘要： The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.