公开(公告)号：EP2342552A1

公开(公告)日：2011-07-13

申请号：EP09822323.3

申请日：2009-10-22

申请人： Life Technologies Corporation

发明人： ROTHBERG, Jonathan, M. ,  SCHULTZ, Jonathan, C. ,  JOHNSON, Kim, L. ,  REARICK, Todd, M. ,  MILGREW, Mark, James ,  MARRAN, David ,  BUSTILLO, James, M.

IPC分类号： G01N27/416 ,  C12M1/34

CPC分类号： G01N27/4145

摘要： The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.

公开(公告)号：EP4220146A1

公开(公告)日：2023-08-02

申请号：EP22188998.3

申请日：2010-05-27

申请人： Life Technologies Corporation

发明人： ROTHBERG, Jonathan M. ,  HINZ, Wolfgang ,  DAVIDSON, John F. ,  VAN OIJEN, Antoine M ,  LEAMON, John H. ,  HUBER, Martin ,  BUSTILLO, James M. ,  MILGREW, Mark, James ,  SCHULTZ, Jonathan ,  MARRAN, David ,  REARICK, Todd ,  JOHNSON, Kim L.

IPC分类号： G01N27/416 ,  C12M1/34 ,  G01N27/414 ,  C12Q1/00

摘要： An apparatus may include an array of sensors, a plurality of microwells, a row addressing circuit, a column addressing circuit, and analog-to-digital converters. Each sensor includes a two-transistor circuit with a chemically-sensitive field effect transistor (chemFET) and a row select transistor and each chemFETs has a floating gate structure and a passivation material located over at least a subset of the floating gate structures. The microwells define a plurality of cavities disposed over at least a portion of the floating gate structures and are of a size and shape capable of retaining analyte therein. The column addressing circuit is suitable for supplying output from each sensor in the array of sensors. The analog-to-digital converters are used for each column in the sensor array to convert an analog output of the column addressing circuit to digital form.

公开(公告)号：EP2435128A2

公开(公告)日：2012-04-04

申请号：EP10780930.3

申请日：2010-05-27

申请人： Life Technologies Corporation

发明人： ROTHBERG, Jonathan M. ,  HINZ, Wolfgang ,  DAVIDSON, John F. ,  VAN OIJEN, Antoine M. ,  LEAMON, John H. ,  HUBER, Martin ,  BUSTILLO, James M. ,  MILGREW, Mark, James ,  SCHULTZ, Jonathan ,  MARRAN, David ,  REARICK, Todd ,  JOHNSON, Kim L.

IPC分类号： A61M37/00 ,  A61B5/145

CPC分类号： G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/27 ,  G01N27/414 ,  Y10T29/49002

摘要： Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.