公开(公告)号：EP3327440B1

公开(公告)日：2020-01-08

申请号：EP17191391.6

申请日：2013-02-13

申请人： Life Technologies Corporation

发明人： VLASSOV, Alexander ,  LI, Mu ,  ZERINGER, Emily ,  CONRAD, Richard

IPC分类号： G01N33/50 ,  G01N33/92

公开(公告)号：EP2839278B1

公开(公告)日：2017-09-27

申请号：EP13706853.2

申请日：2013-02-13

申请人： Life Technologies Corporation

发明人： VLASSOV, Alexander ,  LI, Mu ,  ZERINGER, Emily ,  CONRAD, Richard

IPC分类号： G01N33/50

CPC分类号： G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

摘要： Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

公开(公告)号：EP2839278A1

公开(公告)日：2015-02-25

申请号：EP13706853.2

申请日：2013-02-13

申请人： Life Technologies Corporation

发明人： VLASSOV, Alexander ,  LI, Mu ,  ZERINGER, Emily ,  CONRAD, Richard

IPC分类号： G01N33/50

CPC分类号： G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

摘要： Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

摘要翻译： 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 使用不含体积聚合物来从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤允许外来体分离。 还描述了沉淀后进一步分离外泌体。

公开(公告)号：EP3347491A1

公开(公告)日：2018-07-18

申请号：EP16778500.5

申请日：2016-09-09

申请人： Life Technologies Corporation

发明人： VLASSOV, Alexander ,  LAROCCA, Sarah ,  LI, Mu

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/1003 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N33/56938 ,  G01N33/574 ,  G01N2800/06

摘要： Compositions and methods for nucleic acid isolation from an environmental or biological sample comprising nucleic acid-analysis interferents, particularly from microbiome-containing samples, are provided.

公开(公告)号：EP3327440A1

公开(公告)日：2018-05-30

申请号：EP17191391.6

申请日：2013-02-13

申请人： Life Technologies Corporation

发明人： VLASSOV, Alexander ,  LI, Mu ,  ZERINGER, Emily ,  CONRAD, Richard

IPC分类号： G01N33/50 ,  G01N33/92

摘要： Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.