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1.发明公开MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 有权改良蛋白酶自身VERFAHREN ZU IHRER HERSTELLUNG UND VERWENDUNG
公开(公告)号：EP3040424A1
公开(公告)日：2016-07-06
申请号：EP15192848.8
申请日：2011-02-28
CPC分类号： C12N9/1252
摘要： Methods, compositions, systems, and kits comprising modified proteins, particularly modified nucleic acid-binding proteins and DNA polymerases with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.
摘要翻译： 提供了包含修饰的蛋白质，特别是经修饰的核酸结合蛋白质和具有改变的缓冲性质的DNA聚合酶的方法，组合物，系统和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这种修饰的蛋白质的方法，例如基于离子的核酸测序反应。
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公开(公告)号：EP2952590B1
公开(公告)日：2017-07-26
申请号：EP15178097.0
申请日：2011-06-10
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A method of sequencing a polynucleotide strand may include following operations: (i) providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand, and (ii) successively exposing the polynucleotide strand to the flow of four different dNTPs according to a predetermined ordering. The predetermined ordering may include an alternate ordering which is not a continuous repeat of an ordering of the four different dNTPs.
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公开(公告)号：EP4220146A1
公开(公告)日：2023-08-02
申请号：EP22188998.3
申请日：2010-05-27
发明人： ROTHBERG, Jonathan M. , HINZ, Wolfgang , DAVIDSON, John F. , VAN OIJEN, Antoine M , LEAMON, John H. , HUBER, Martin , BUSTILLO, James M. , MILGREW, Mark, James , SCHULTZ, Jonathan , MARRAN, David , REARICK, Todd , JOHNSON, Kim L.
IPC分类号： G01N27/416 , C12M1/34 , G01N27/414 , C12Q1/00
摘要： An apparatus may include an array of sensors, a plurality of microwells, a row addressing circuit, a column addressing circuit, and analog-to-digital converters. Each sensor includes a two-transistor circuit with a chemically-sensitive field effect transistor (chemFET) and a row select transistor and each chemFETs has a floating gate structure and a passivation material located over at least a subset of the floating gate structures. The microwells define a plurality of cavities disposed over at least a portion of the floating gate structures and are of a size and shape capable of retaining analyte therein. The column addressing circuit is suitable for supplying output from each sensor in the array of sensors. The analog-to-digital converters are used for each column in the sensor array to convert an analog output of the column addressing circuit to digital form.
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公开(公告)号：EP2435128A2
公开(公告)日：2012-04-04
申请号：EP10780930.3
申请日：2010-05-27
发明人： ROTHBERG, Jonathan M. , HINZ, Wolfgang , DAVIDSON, John F. , VAN OIJEN, Antoine M. , LEAMON, John H. , HUBER, Martin , BUSTILLO, James M. , MILGREW, Mark, James , SCHULTZ, Jonathan , MARRAN, David , REARICK, Todd , JOHNSON, Kim L.
CPC分类号： G01N27/4145 , C12Q1/6869 , C12Q1/6874 , G01N27/27 , G01N27/414 , Y10T29/49002
摘要： Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
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5.发明公开
公开(公告)号：EP2952590A1
公开(公告)日：2015-12-09
申请号：EP15178097.0
申请日：2011-06-10
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A method of sequencing a polynucleotide strand may include following operations: (i) providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand, and (ii) successively exposing the polynucleotide strand to the flow of four different dNTPs according to a predetermined ordering. The predetermined ordering may include an alternate ordering which is not a continuous repeat of an ordering of the four different dNTPs.
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公开(公告)号：EP2580353B1
公开(公告)日：2015-07-29
申请号：EP11793237.6
申请日：2011-06-10
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A method of sequencing a polynucleotide strand may include following operations: (i) providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand, and (ii) successively exposing the polynucleotide strand to the flow of four different dNTPs according to a predetermined ordering. The predetermined ordering may include an alternate ordering which is not a continuous repeat of an ordering of the four different dNTPs.
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7.发明公开
公开(公告)号：EP2580353A2
公开(公告)日：2013-04-17
申请号：EP11793237.6
申请日：2011-06-10
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A method of sequencing a polynucleotide strand may include following operations: (i) providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand, and (ii) successively exposing the polynucleotide strand to the flow of four different dNTPs according to a predetermined ordering. The predetermined ordering may include an alternate ordering which is not a continuous repeat of an ordering of the four different dNTPs.
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