公开(公告)号：EP0852336A1

公开(公告)日：1998-07-08

申请号：EP97310655.2

申请日：1997-12-30

申请人： Lifescan, Inc.

发明人： Douglas, joel ,  Kiser, Ernest ,  Tomasco, Michael F. ,  Dato, Remedios ,  Rice, Edward G. ,  Tuohy, Deborah P. ,  Maxson, Mark ,  Witko, Zbigniew ,  Segelke, Scott

IPC分类号： G01N33/558 ,  C12Q1/54

CPC分类号： G01N33/66 ,  C12Q1/54 ,  G01N33/521 ,  G01N33/523 ,  G01N33/526 ,  G01N33/558

摘要： A multilayer reagent test strip measures the concentration of analyte in a liquid sample that is applied to it. The sample is guided to a number of assay areas arrayed along the strip, where the analyte can react with a reagent to cause a color change. Each assay area also includes an inhibitor for the color-change reaction. The inhibitor concentration increases in successive assay areas; thus, the number of areas that change color is a measure of the analyte concentration. The test strip is particularly adapted for measuring glucose in a whole blood sample. In a preferred embodiment, the sample is guided to the assay areas along a path formed by crushing selected areas of a membrane, and the assay areas are uncrushed areas of the membrane.

摘要翻译： 多层试剂测试条测量施加于其上的液体样品中分析物的浓度。 将样品引导到沿着条带排列的多个测定区域，其中分析物可以与试剂反应以引起颜色变化。 每个测定区域还包括用于变色反应的抑制剂。 连续测定区域的抑制剂浓度增加; 因此，改变颜色的区域的数量是分析物浓度的量度。 测试条特别适用于测量全血样品中的葡萄糖。 在优选的实施方案中，将样品沿着通过破碎膜的选定区域形成的路径被引导到测定区域，并且测定区域是膜的未破碎区域。

公开(公告)号：EP0826777A1

公开(公告)日：1998-03-04

申请号：EP97306765.5

申请日：1997-09-02

申请人： Lifescan, Inc.

发明人： Kiser, Ernest J. ,  Tomasco, Michael F. ,  Rice, Edward G. ,  Yu, Yeung S.

IPC分类号： C12Q1/54 ,  G01N33/52

CPC分类号： G01N33/521 ,  C12Q1/54 ,  G01N33/523 ,  G01N33/526 ,  G01N33/558

摘要： A chemical timer for a direct-reading reagent test strip changes color a predetermined time after a biological fluid is applied to the strip. The strip measures the concentration of an analyte in the fluid. The timer is a dry coating of an indicator, an enzyme-containing reagent that when hydrated can react with glucose to change the color of the indicator, an inhibitor to inhibit the change in color of the indicator, glucose, and optionally, an aldose that does not react with the enzyme in the reagent. Preferably, the reagent and glucose are present in excess in the coating, and the time it takes for the timer color to change can be controlled by the inhibitor concentration. The aldose provides timer stability, probably by interfering with glycosylation by the glucose in the dry state.

摘要翻译： 用于直接读取试剂测试条的化学计时器在将生物流体施加到条带之后的预定时间内改变颜色。 条带测量流体中分析物的浓度。 定时器是指示剂的干燥涂层，含酶试剂当水合时可与葡萄糖反应以改变指示剂的颜色，抑制剂可抑制指示剂，葡萄糖和任选的醛糖变色， 不与试剂中的酶反应。 优选地，试剂和葡萄糖在涂层中过量存在，并且定时器颜色改变所需的时间可以通过抑制剂浓度来控制。 醛糖提供定时器稳定性，可能通过在干燥状态下干扰葡萄糖的糖基化。

公开(公告)号：EP0759555A2

公开(公告)日：1997-02-26

申请号：EP96305720.3

申请日：1996-08-02

申请人： LIFESCAN, INC.

发明人： Douglas, Joel ,  Tomasco, Michael F. ,  Kiser, Ernest ,  Dato, Remedios ,  Rice, Edward G. ,  Tuohy, Deborah P. ,  Maxson, Mark

IPC分类号： G01N33/52

CPC分类号： G01N33/526 ,  C12Q1/54

摘要： A multilayer reagent test strip measures the concentration of analyte in a liquid sample that is applied to it. The sample is guided to a number of assay areas arrayed along the strip, where the analyte can react with a reagent to cause a color change. Each assay area also includes an inhibitor for the color-change reaction. The inhibitor concentration increases in successive assay areas; thus, the number of areas that change color is a measure of the analyte concentration. The test strip is particularly adapted for measuring glucose in a whole blood sample. In a preferred embodiment, the sample is guided to the assay areas along a path formed by crushing selected areas of a membrane, and the assay areas are uncrushed areas of the membrane.

摘要翻译： 多层试剂测试条测量应用于其上的液体样品中分析物的浓度。 将样品引导至沿着条带排列的多个分析区域，其中分析物可以与试剂反应以引起颜色变化。 每个测定区域还包括用于颜色变化反应的抑制剂。 在连续测定区域中抑制剂浓度增加; 因此，改变颜色的区域的数量是分析物浓度的量度。 测试条特别适用于测量全血样品中的葡萄糖。 在一个优选的实施方案中，样品沿着通过压碎膜的选定区域而形成的路径被引导至测定区域，并且测定区域是膜的未压碎区域。

公开(公告)号：EP0826777B1

公开(公告)日：2001-08-01

申请号：EP97306765.5

申请日：1997-09-02

申请人： Lifescan, Inc.

发明人： Kiser, Ernest J. ,  Tomasco, Michael F. ,  Rice, Edward G. ,  Yu, Yeung S.

IPC分类号： C12Q1/54 ,  G01N33/52

CPC分类号： G01N33/521 ,  C12Q1/54 ,  G01N33/523 ,  G01N33/526 ,  G01N33/558

公开(公告)号：EP0475692A1

公开(公告)日：1992-03-18

申请号：EP91308178.2

申请日：1991-09-06

申请人： LIFESCAN, INC.

发明人： Kiser, Ernest J. ,  Rice, Edward G. ,  Tomasco, Michael F.

IPC分类号： G01N33/52 ,  C12Q1/54 ,  G01N33/66

CPC分类号： G01N33/48 ,  C12Q1/54 ,  G01N33/521 ,  G01N33/523 ,  Y10T436/25375

摘要： A separation matrix impregnated with a separating agent separates red blood cells or hemoglobin from whole blood. Upon separation, an impregnated test reagent reacts with the separated substantially clear component fluid to form a colored product which enables a visual or meter test for analyte levels in whole blood. Such a test is appropriate for, among other things measuring glucose, cholesterol, or alcohol levels in whole blood samples. A visual meter is disclosed which allows sample to be applied to one side of the strip, and reading takes place on the opposite side, after separation of red blood cells and reaction to test reagent. Also, a useful test strip format is disclosed.

摘要翻译： 用分离剂浸渍的分离基质将红细胞或血红蛋白与全血分离。 在分离时，浸渍的测试试剂与分离的基本上透明的组分流体反应以形成有色产物，其能够对全血中的分析物水平进行视觉或仪表测试。 这样的测试适用于测量全血样品中的葡萄糖，胆固醇或酒精水平。 公开了一种视觉仪表，其允许将样品施加到条带的一侧，并且在分离红细胞并与测试试剂反应之后，在相对侧进行读数。 此外，公开了一种有用的测试条格式。

公开(公告)号：EP0415679A3

公开(公告)日：1991-07-03

申请号：EP90309344.1

申请日：1990-08-24

申请人： LIFESCAN, INC.

发明人： Kiser, Ernest J. ,  Rice, Edward G. ,  Tomasco, Michael F.

IPC分类号： G01N33/52

CPC分类号： C12Q1/54 ,  G01N33/48 ,  G01N33/521

摘要： A separation matrix impregnated with a separating agent separates red blood cells or hemoglobin from whole blood. Upon separation, an impregnated test reagent reacts with the separated substantially clear component fluid to form a colored product which enables a visual or meter test for analyte levels in whole blood. Such a test is appropriate for, among other things measuring glucose, cholesterol, or alcohol levels in whole blood samples.

公开(公告)号：EP0852336B1

公开(公告)日：2004-04-14

申请号：EP97310655.2

申请日：1997-12-30

申请人： Lifescan, Inc.

发明人： Douglas, joel ,  Kiser, Ernest ,  Tomasco, Michael F. ,  Dato, Remedios ,  Rice, Edward G. ,  Tuohy, Deborah P. ,  Maxson, Mark ,  Witko, Zbigniew ,  Segelke, Scott

IPC分类号： G01N33/558 ,  C12Q1/54

CPC分类号： G01N33/66 ,  C12Q1/54 ,  G01N33/521 ,  G01N33/523 ,  G01N33/526 ,  G01N33/558

公开(公告)号：EP0475692B1

公开(公告)日：1995-05-31

申请号：EP91308178.2

申请日：1991-09-06

申请人： LIFESCAN, INC.

发明人： Kiser, Ernest J. ,  Rice, Edward G. ,  Tomasco, Michael F.

IPC分类号： G01N33/52 ,  C12Q1/54 ,  G01N33/66

CPC分类号： G01N33/48 ,  C12Q1/54 ,  G01N33/521 ,  G01N33/523 ,  Y10T436/25375

公开(公告)号：EP0415679A2

公开(公告)日：1991-03-06

申请号：EP90309344.1

申请日：1990-08-24

申请人： LIFESCAN, INC.

发明人： Kiser, Ernest J. ,  Rice, Edward G. ,  Tomasco, Michael F.

IPC分类号： G01N33/52

CPC分类号： C12Q1/54 ,  G01N33/48 ,  G01N33/521

摘要： A separation matrix impregnated with a separating agent separates red blood cells or hemoglobin from whole blood. Upon separation, an impregnated test reagent reacts with the separated substantially clear component fluid to form a colored product which enables a visual or meter test for analyte levels in whole blood. Such a test is appropriate for, among other things measuring glucose, cholesterol, or alcohol levels in whole blood samples.

摘要翻译： 用分离剂浸渍的分离基质将红细胞或血红蛋白与全血分离。 在分离时，浸渍的测试试剂与分离的基本上透明的组分流体反应以形成有色产物，其能够对全血中的分析物水平进行视觉或仪表测试。 这样的测试适用于测量全血样品中的葡萄糖，胆固醇或酒精水平。