公开(公告)号：EP0914611A1

公开(公告)日：1999-05-12

申请号：EP97924507.0

申请日：1997-04-18

申请人： MERCK & CO., INC.

发明人： ROSENBLUM, Charles, I. ,  VAN DER PLOEG, Leonardus ,  QURESHI, Sajjad, A. ,  CULLY, Doris, F. ,  HESS, John, W. ,  TOTA, Michael, R. ,  CHEN, Fang

IPC分类号： C12N15 ,  C07K14 ,  C12N1 ,  C12N5 ,  C12Q1 ,  G01N33

CPC分类号： C07K14/715 ,  C07K14/4705 ,  C12Q1/6897

摘要： A trans-activation assay for leptin is described. Leptin response elements are located proximal to a promoter, and the promoter region is operatively linked to a reporter gene. When leptin binds with the receptor, the reporter gene is transcribed.

公开(公告)号：EP0935753A1

公开(公告)日：1999-08-18

申请号：EP97946307.0

申请日：1997-10-27

申请人： MERCK & CO., INC.

发明人： ROSENBLUM, Charles, I. ,  VONGSKIERTTIKACHOR, Aurawan

IPC分类号： C12Q1 ,  G01N33

CPC分类号： C12Q1/6897 ,  G01N33/74 ,  G01N2333/61 ,  G01N2500/00

摘要： A stably transformed cell line is provided for the identification of growth hormone mimetics wherein the cell line is transformed to express a reporter gene construct comprised of a promoter and a growth hormone response element operatively linked to a reporter gene.