公开(公告)号：EP0914611A1

公开(公告)日：1999-05-12

申请号：EP97924507.0

申请日：1997-04-18

申请人： MERCK & CO., INC.

发明人： ROSENBLUM, Charles, I. ,  VAN DER PLOEG, Leonardus ,  QURESHI, Sajjad, A. ,  CULLY, Doris, F. ,  HESS, John, W. ,  TOTA, Michael, R. ,  CHEN, Fang

IPC分类号： C12N15 ,  C07K14 ,  C12N1 ,  C12N5 ,  C12Q1 ,  G01N33

CPC分类号： C07K14/715 ,  C07K14/4705 ,  C12Q1/6897

摘要： A trans-activation assay for leptin is described. Leptin response elements are located proximal to a promoter, and the promoter region is operatively linked to a reporter gene. When leptin binds with the receptor, the reporter gene is transcribed.

公开(公告)号：EP0870199A1

公开(公告)日：1998-10-14

申请号：EP96943623.0

申请日：1996-12-10

申请人： MERCK & CO., INC.

发明人： CHAUNG, Lee-Yuh ,  FEIGHNER, Scott, D. ,  HOWARD, Andrew, D. ,  PONG, Sheng, S. ,  VAN DER PLOEG, Leonardus

IPC分类号： G01N33 ,  C07K14 ,  C12N15 ,  C12Q1 ,  C12R1

CPC分类号： G01N33/74 ,  C07K14/723 ,  C12Q1/6897 ,  G01N2333/726 ,  G01N2500/10

摘要： An assay for the detection of growth hormone secretagogue receptors and growth hormone secretagogue related receptors is described. As these receptors are a member of the G protein coupled receptors, a subunit of the G protein must be present in order for expression to be detected. A similar assay is described where the presence of growth hormone secretagogues are detected.

公开(公告)号：EP0870199B1

公开(公告)日：2006-07-26

申请号：EP96943623.7

申请日：1996-12-10

申请人： Merck & Co., Inc.

发明人： CHAUNG, Lee-Yuh ,  FEIGHNER, Scott, D. ,  HOWARD, Andrew, D. ,  PONG, Sheng, S. ,  VAN DER PLOEG, Leonardus

IPC分类号： G01N33/566 ,  C12N15/87 ,  C12N15/63 ,  C07K14/715 ,  C12Q1/02

CPC分类号： G01N33/74 ,  C07K14/723 ,  C12Q1/6897 ,  G01N2333/726 ,  G01N2500/10

摘要： An assay for the detection of growth hormone secretagogue receptors and growth hormone secretagogue related receptors is described. As these receptors are a member of the G protein coupled receptors, a subunit of the G protein must be present in order for expression to be detected. A similar assay is described where the presence of growth hormone secretagogues are detected.

公开(公告)号：EP0948595A1

公开(公告)日：1999-10-13

申请号：EP97949741.0

申请日：1997-11-26

申请人： MERCK & CO., INC.

发明人： FONG, Tung, M. ,  HUANG, Ruey-Ruey, C. ,  VAN DER PLOEG, Leonardus

IPC分类号： C12N15 ,  C07K14 ,  C12N1 ,  C12N5 ,  C12Q1 ,  G01N33

CPC分类号： G01N33/74 ,  C07K14/5759 ,  G01N33/5005 ,  G01N33/6893 ,  G01N2500/00

摘要： Mutant ob receptors have been made which a) lack a functional first CK-F3 domain; b) lack a functional second CK-F3 domain or c) lack a functional intracellular domain. These receptors may be used in various assays, such as a transactivation assay to identify novel ligands.

公开(公告)号：EP0876402B1

公开(公告)日：2004-10-13

申请号：EP96945183.0

申请日：1996-12-10

申请人： MERCK & CO., INC.

发明人： ARENA, Joseph, P. ,  CULLY, Doris, F. ,  FEIGHNER, Scott, D. ,  HOWARD, Andrew, D. ,  LIBERATOR, Paul, A. ,  SCHAEFFER, James, M. ,  VAN DER PLOEG, Leonardus

IPC分类号： C07K14/705 ,  C07K14/72 ,  C07K14/435 ,  C07K14/60 ,  C07K14/61 ,  C07K14/47 ,  C12N15/12 ,  C12N15/09 ,  C12N15/10 ,  C12N15/00 ,  C12N5/10

CPC分类号： C07K14/723 ,  A61K38/00 ,  C07B59/002

摘要： Human, swine and rat growth hormone secretagogue receptors have been isolated, cloned and sequenced. Growth hormone secretagogue receptors are new members of the G-protein family of receptors. The growth hormone secretagogue receptors may be used to screen and identify compounds which bind to the growth hormone secretagogue receptor. Such compounds may be used in the treatment of conditions which occur when there is a shortage of growth hormone, such as observed in growth hormone deficient children, elderly patients with musculoskeletal impairment and recovering from hip fracture and osteoporosis.

摘要翻译： 已经分离，克隆和测序了人，猪和大鼠生长激素促分泌素受体。 生长激素促分泌素受体是G蛋白家族受体的新成员。 生长激素促分泌素受体可用于筛选和鉴定与生长激素促分泌素受体结合的化合物。 这类化合物可用于治疗生长激素短缺时发生的病症，例如在生长激素缺乏的儿童，肌肉骨骼损伤的老年患者以及从髋部骨折和骨质疏松症中恢复中观察到的病症。

公开(公告)号：EP1037913A1

公开(公告)日：2000-09-27

申请号：EP98964016.4

申请日：1998-12-17

申请人： Merck & Co., Inc. (a New Jersey corp.)

发明人： HOWARD, Andrew ,  CASCIERI, Margaret ,  SMITH, Roy ,  SULLIVAN, Kathleen ,  TAN, Carina ,  VAN DER PLOEG, Leonardus ,  LYNCH, Kevin

IPC分类号： C07K14/00 ,  C07K14/705 ,  C12N5/16 ,  C12N15/12 ,  C12N15/63 ,  G01N33/53 ,  G01N33/566

CPC分类号： C07K14/72 ,  A61K38/00

摘要： A new galanin receptor, GALR3, is described. Also provided are nucleic acids encoding same and various assays to identify ligands particular to said receptor. Ligands so identified are useful for the treatment of obesity, treatment of pain, and treatment of cognitive disorders.

公开(公告)号：EP0876402A1

公开(公告)日：1998-11-11

申请号：EP96945183.0

申请日：1996-12-10

申请人： MERCK & CO., INC.

发明人： ARENA, Joseph, P. ,  CULLY, Doris, F. ,  FEIGHNER, Scott, D. ,  HOWARD, Andrew, D. ,  LIBERATOR, Paul, A. ,  SCHAEFFER, James, M. ,  VAN DER PLOEG, Leonardus

IPC分类号： G01N33 ,  C07B59 ,  C07K14 ,  C12N1 ,  C12N5 ,  C12N15 ,  A61K38

CPC分类号： C07K14/723 ,  A61K38/00 ,  C07B59/002

摘要： Human, swine and rat growth hormone secretagogue receptors have been isolated, cloned and sequenced. Growth hormone secretagogue receptors are new members of the G-protein family of receptors. The growth hormone secretagogue receptors may be used to screen and identify compounds which bind to the growth hormone secretagogue receptor. Such compounds may be used in the treatment of conditions which occur when there is a shortage of growth hormone, such as observed in growth hormone deficient children, elderly patients with musculoskeletal impairment and recovering from hip fracture and osteoporosis.