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    Cloning and expression of acidic fibroblast growth factor
    3.
    发明公开
    Cloning and expression of acidic fibroblast growth factor 失效
    Klonierung und Expression des sauren Fibroblastenwachstumsfaktors。

    公开(公告)号：EP0259953A1

    公开(公告)日：1988-03-16

    申请号：EP87306066.9

    申请日：1987-07-09

    申请人： Merck & Co., Inc.

    发明人： Linemeyer, David L. ,  Kelly, Linda J. ,  Thomas, Kenneth A., Jr. ,  Gimenez-Gallego, Guillermo

    IPC分类号： C12N15/12 ,  C07K13/00

    CPC分类号： C07K14/501 ,  A61K38/00

    摘要： Unique genes coding for the amino acid sequence of bovine acidic fibroblast growth factor (aFGF) and human aFGF are constructed. The bovine gene is derived from reverse translation of the aFGF amino acid sequence while the human gene is derived by specific point mutations of the bovine gene. Each gene construct is inserted into an expression vector which is used to transform an appropriate host. The transformed host cells produce recombinant aFGF (r-aFGF), human or bovine, which is purified and has an activity equivalent to the native protein.

    摘要翻译： 构建编码牛酸性成纤维细胞生长因子（aFGF）和人aFGF的氨基酸序列的唯一基因。 牛基因来自aFGF氨基酸序列的反向翻译，而人基因是通过牛基因的特异性点突变得到的。 将每个基因构建体插入到用于转化适当宿主的表达载体中。 转化的宿主细胞产生重组的aFGF（r-aFGF），人或牛，其被纯化并具有与天然蛋白质相当的活性。

    Mutant acidic fibroblast growth factor
    7.
    发明公开
    Mutant acidic fibroblast growth factor 失效
    突变型ACIDIC FIBROBLAST生长因子

    公开(公告)号：EP0319052A3

    公开(公告)日：1990-04-25

    申请号：EP88202306.2

    申请日：1988-10-14

    申请人： Merck & Co., Inc.

    发明人： Thomas Jnr, Kenneth A. ,  Linemeyer, David L.

    IPC分类号： C12N15/12 ,  C12P21/02

    CPC分类号： C07K14/501 ,  A61K38/00

    摘要： Novel genes coding for mutant bovine and human aFGF are constructed. The unique genes are derived from genes encoding recombinant bovine and human aFGF by specific point mutations. Each gene construct is inserted into an expression vector which is used to transform an appropriate host. The transformed host cells produce unique mutant recobinant aFGF, human or bovine, which is purified and has enhanced or improved biological activity in the absence of heparin compared to the unmutated forms.

    Method of enhancing acidic fibroblast growth factor expression
    10.
    发明公开
    Method of enhancing acidic fibroblast growth factor expression 失效
    Methode zur Steigerung der Expression des sauren Fibroblast-Wachstumsfaktors。

    公开(公告)号：EP0392605A1

    公开(公告)日：1990-10-17

    申请号：EP90200836.6

    申请日：1990-04-06

    申请人： Merck & Co., Inc.

    发明人： Linemeyer, David L. ,  Thomas, Kenneth A., Jr.

    IPC分类号： C12N15/16 ,  C12P21/02 ,  C12N15/70

    CPC分类号： C07K14/501

    摘要： A unique double cistron expression vector for the enhanced expression of recombinant human acidic fibroblast growth factor (r-haFGF) is disclosed. The double cistron expression vector results in about a 5 to 13-fold increase in the expression of r-haFGF.

    摘要翻译： 公开了用于增强重组人酸性成纤维细胞生长因子（r-haFGF）表达的独特的双顺反子表达载体。 双顺反子表达载体导致r-haFGF表达增加约5至13倍。