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1.发明授权
公开(公告)号：EP0710285B1
公开(公告)日：2001-04-11
申请号：EP94920953.0
申请日：1994-06-17
IPC分类号： C12N15/15 , C07K14/815 , C07K19/00 , C12N15/62 , A61K38/58 , C12N15/70 , C12N15/85 , C12N1/21 , C12N5/16
CPC分类号： C07K14/005 , A61K38/00 , C07K14/815 , C07K2319/02 , C12N2760/20222
摘要： The present invention relates to analogues of anti-thrombin polypeptides and to a process for their preparation. The analogues have been obtained by a C-terminal modification of anti-thrombin polypeptides isolated from the leech Hirudinaria manillensis and are characterized by an improved biological activity in comparison to the polypeptides from which they are derived. The analogues of the invention may be prepared by: 1) isolating the anti-thrombin polypeptide from the tissue or secretions of the leech Hirudinaria manillensis and 2) subsequently modifying the C-terminal end of such polypeptide. Alternatively, the analogues may also be synthetized by recombinant DNA methods. According to this latter aspect, the invention provides DNA sequences, expression vectors and host cell lines for the recombinant preparation of the analogues. The analogues of the invention find a useful application in the treatment of venous thrombosis, vascular shunt occlusion and thrombin-induced disseminated intravascular coagulation.
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2.发明公开
公开(公告)号：EP0710285A1
公开(公告)日：1996-05-08
申请号：EP94920953.0
申请日：1994-06-17
CPC分类号： C07K14/005 , A61K38/00 , C07K14/815 , C07K2319/02 , C12N2760/20222
摘要： The present invention relates to analogues of anti-thrombin polypeptides and to a process for their preparation. The analogues have been obtained by a C-terminal modification of anti-thrombin polypeptides isolated from the leech Hirudinaria manillensis and are characterized by an improved biological activity in comparison to the polypeptides from which they are derived. The analogues of the invention may be prepared by: 1) isolating the anti-thrombin polypeptide from the tissue or secretions of the leech Hirudinaria manillensis and 2) subsequently modifying the C-terminal end of such polypeptide. Alternatively, the analogues may also be synthetized by recombinant DNA methods. According to this latter aspect, the invention provides DNA sequences, expression vectors and host cell lines for the recombinant preparation of the analogues. The analogues of the invention find a useful application in the treatment of venous thrombosis, vascular shunt occlusion and thrombin-induced disseminated intravascular coagulation.
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3.发明公开RECOMBINANT PROCESS FOR THE PRODUCTION IN PSEUDOMONAS PUTIDA OF THE CYTOCHROME C 551? OF PSEUDOMONAS AERUGINOSA 失效对于生产细胞色素C551重组方法从铜绿假单胞菌恶臭假单胞菌
公开(公告)号：EP0894138A1
公开(公告)日：1999-02-03
申请号：EP97907089.0
申请日：1997-03-10
发明人： SILVESTRINI, Maria, Chiara +di , CUTRUZZOLA', Francesca , CIABATTI, Ilaria , ZENNARO, Elisabetta , VISCO, Carlo , DISCEPOLO, Massimo
CPC分类号： C07K14/80
摘要： The invention relates to a recombinant process for the production of a hemoprotein having the ability of transporting electrons and comprising the sequence of cytochrome C551 of Pseudomonas aeruginosa, characterized in that this hemoprotein is produced in Pseudomonas putida. The hemoprotein obtained through the process of the invention includes the natural form of cytochrome C551, as well as its precursors characterized by the presence of all or a portion, typically an N-terminal part, of a signal sequence. The hemoprotein object of the invention can be prepared in the following way: (a) providing a host, transformed with an expression vector comprising a DNA sequence which encodes the hemoprotein, in such conditions that said hemoprotein is expressed and (b) isolating or purifying said hemoprotein. Hence further objects of this invention also includes: an expression vector comprising a DNA sequence which encodes a hemoprotein of the invention; a host transformed with a suitable expression vector according to the invention; and a DNA molecule of natural or synthetic origin, comprising a sequence encoding the hemoprotein according to the present invention. A hemoprotein according to the invention finds useful applications in the diagnostic field, for example as chromogenic substrate for peroxidase or in electrochemical studies, in which it is used for the detection, measurement and control of the reactions of electronic transfer between oxidoreductive proteins and electrode.
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