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    Method for producing L-threonine
    1.
    发明公开
    Method for producing L-threonine 失效
    Verfahren zur Herstellung von L-Threonin。

    公开(公告)号：EP0312089A2

    公开(公告)日：1989-04-19

    申请号：EP88117119.3

    申请日：1988-10-14

    申请人： MITSUBISHI PETROCHEMICAL CO., LTD.

    发明人： Terasawa, Masato c/o Central Research Lab. ,  Nara, Terukazu c/o Central Research Lab. ,  Fukushima, Makiko c/o Central Research Lab. ,  Satoo, Yukie c/o Central Research Lab. ,  Shimazu, Mitsunobu c/o Central Research Lab. ,  Yukawa, Hideaki c/o Central Research Lab. ,  Kurusu, Yasurou c/o Central Research Lab. ,  Kohama, Keiko c/o Central Research Lab.

    IPC分类号： C12P13/08 ,  C12N15/52 ,  C12N15/77 ,  C12N1/20

    CPC分类号： C12P13/08 ,  C12N15/52 ,  C12N15/77 ,  Y10S435/84

    摘要： A method for producing L-threonine, which comprises subjecting at least L- or DL-aspartic acid or a salt thereof to enzymatic reaction according to the reaction system not accompanied with growth of microorganism cells in an aqueous solution in the presence of a microorganism and collecting L-threonine formed, wherein the micro­organism is a biotin-requiring microorganism for the growth belonging to coryneform bacterium; a plasmid comprising a DNA fragment containing at least a gene encoding biosynthesis of threonine which can be expressed within a biotin-requiring microorganism cell for the growth belonging to coryneform bacterium and a DNA fragment containing a gene encoding autonomous replica­tion within coryneform bacterium cell; and a biotin-­requiring microorganism for the growth belonging to coryneform bacterium which has been transformed with the plasmid described above, both of which are employed in the present method.
    According to the present invention, L-threonine can be produced with good yield, and further since production management becomes extremely easy without requiring cumbersome operation such as sterilization of the medium, etc. as in the fermentation method, L-threonine can be produced inexpensively in industry.

    摘要翻译： 一种L-苏氨酸的制造方法，其特征在于，在微生物的存在下，根据不伴随微生物细胞在水溶液中生长的反应体系，使至少L-或DL-天冬氨酸或其盐进行酶反应， 收集形成的L-苏氨酸，其中微生物是属于棒状细菌的生长物的生物素需要微生物; 包含至少含有编码苏氨酸生物合成基因的DNA片段的质粒，该氨基酸可在属于棒杆菌型细菌的生长物的生物素需要微生物细胞内表达，以及含有在棒状细菌细胞内编码自主复制的基因的DNA片段; 以及已经用上述质粒转化的属于棒状杆菌型细菌的生长物的生物素需要微生物，两者均用于本发明的方法。 根据本发明，可以以良好的产率制造L-苏氨酸，进一步由于生产管理变得非常容易，而不需要诸如发酵方法的介质等的繁琐操作等麻烦的操作，所以可以廉价地生产L-苏氨酸 在行业。