-
公开(公告)号：EP3199631B1
公开(公告)日：2019-01-30
申请号：EP17160119.8
申请日：2001-11-29
-
公开(公告)号：EP3199631A1
公开(公告)日：2017-08-02
申请号：EP17160119.8
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie (EMBL)
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K47/549 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
摘要翻译： 双链RNA（dsRNA）通过称为RNA干扰（RNAi）的过程在许多生物体中诱导序列特异性转录后基因沉默。 使用果蝇体外系统，我们证明19-23 nt短的RNA片段是RNAi的序列特异性介质。 短干扰RNA（siRNA）由来自长dsRNA的RNase III样加工反应产生。 具有突出3'末端的化学合成的siRNA双链体在裂解物中介导有效靶RNA裂解，并且裂解位点位于导向siRNA跨越的区域的中心附近。 此外，我们提供的证据表明，dsRNA加工的方向决定了正义或反义目标RNA是否可以被产生的siRNP复合物切割。
-
3.发明公开INDUCIBLE SMALL INTERFERING RNA (SIRNA) EXPRESSION CONSTRUCTS FOR TARGETED GENE SILENCING 有权基因诱导siRNA表达构建了靶向基因沉默
公开(公告)号：EP1583832A2
公开(公告)日：2005-10-12
申请号：EP04702315.5
申请日：2004-01-15
IPC分类号： C12N15/11
CPC分类号： C12N15/111 , A01K2217/05 , A01K2217/075 , A61K38/00 , A61K48/0058 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： The invention relates to vectors for the inducible expression of RNA molecules in eukaryotic, particularly mammalian cells and transgenic animals.
-
公开(公告)号：EP1407044B1
公开(公告)日：2007-09-19
申请号：EP01985833.1
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie
IPC分类号： C12Q1/68
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
-
公开(公告)号：EP1407044B2
公开(公告)日：2017-11-15
申请号：EP01985833.1
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
-
公开(公告)号：EP1520022B1
公开(公告)日：2015-07-22
申请号：EP03763812.9
申请日：2003-07-10
发明人： TUSCHL, Thomas , MARTINEZ, Javier , PATKANIOWSKA, Agnieszka , URLAUB, Henning , LÜHRMANN, Reinhard
CPC分类号： C12N15/113 , A61K47/549 , A61K47/557 , C07K14/4705 , C07K19/00 , C12N15/111 , C12N2310/14 , C12N2310/351 , C12N2310/3513 , C12N2320/30 , C12N2320/51 , C12N2330/30
摘要： The present invention relates to sequence and structural features of single-stranded (ss) RNA molecules required to mediate target-specific nucleic acid modifications by RNA-interference (RNAi), such as target mRNA degradation and/or DNA methylation.
-
7.发明授权INDUCIBLE SMALL INTERFERING RNA (SIRNA) EXPRESSION CONSTRUCTS FOR TARGETED GENE SILENCING 有权基因诱导siRNA表达构建了靶向基因沉默
公开(公告)号：EP1583832B1
公开(公告)日：2010-12-01
申请号：EP04702315.5
申请日：2004-01-15
IPC分类号： C12N15/11
CPC分类号： C12N15/111 , A01K2217/05 , A01K2217/075 , A61K38/00 , A61K48/0058 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： The invention relates to vectors for the inducible expression of RNA molecules in eukaryotic, particularly mammalian cells and transgenic animals.
-
-
-
-
-
-
搜索结果
7 条记录 (耗时 0.024 秒)
