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公开(公告)号：EP3199631A1
公开(公告)日：2017-08-02
申请号：EP17160119.8
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie (EMBL)
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K47/549 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
摘要翻译： 双链RNA（dsRNA）通过称为RNA干扰（RNAi）的过程在许多生物体中诱导序列特异性转录后基因沉默。 使用果蝇体外系统，我们证明19-23 nt短的RNA片段是RNAi的序列特异性介质。 短干扰RNA（siRNA）由来自长dsRNA的RNase III样加工反应产生。 具有突出3'末端的化学合成的siRNA双链体在裂解物中介导有效靶RNA裂解，并且裂解位点位于导向siRNA跨越的区域的中心附近。 此外，我们提供的证据表明，dsRNA加工的方向决定了正义或反义目标RNA是否可以被产生的siRNP复合物切割。
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公开(公告)号：EP3199631B1
公开(公告)日：2019-01-30
申请号：EP17160119.8
申请日：2001-11-29
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公开(公告)号：EP2428569B1
公开(公告)日：2018-05-23
申请号：EP11175595.5
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , DAMMANN, Jutta , RAUHUT, Reinhard
IPC分类号： A61K38/00 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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公开(公告)号：EP1430128A2
公开(公告)日：2004-06-23
申请号：EP02782801.1
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , MEYER, Jutta , RAUHUT, Reinhard
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
摘要翻译： 在秀丽隐杆线虫中，lin-4和let-7分别编码22和21个核苷酸的RNA，其作为发育时机的关键调节剂。 因为这些短RNA的出现在发育过程中受到调节，所以它们也被称为“小时间RNA”（stRNA）。 我们显示，更多的21和22-nt表达的RNA，称为microRNA（miRNA），存在于无脊椎动物和脊椎动物中，并且与let-7 stRNA相似的这些新型RNA中的一些也是高度保守的。 这表明由小RNA介导的序列特异性转录后调控机制比以前赞赏更为普遍。
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公开(公告)号：EP2386637B1
公开(公告)日：2018-05-16
申请号：EP11175587.2
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , DAMMANN, Jutta , RAUHUT, Reinhard
IPC分类号： A61K38/00 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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公开(公告)号：EP1407044B2
公开(公告)日：2017-11-15
申请号：EP01985833.1
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
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公开(公告)号：EP2428570B1
公开(公告)日：2018-05-23
申请号：EP11175598.9
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , DAMMANN, Jutta , RAUHUT, Reinhard
IPC分类号： A61K38/00 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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公开(公告)号：EP1430128B1
公开(公告)日：2018-04-25
申请号：EP02782801.1
申请日：2002-09-27
发明人： TUSCHL, Thomas , LAGOS-QUINTANA, Mariana , LENDECKEL, Winfried , DAMMANN, Jutta , RAUHUT, Reinhard
IPC分类号： C12N15/113 , A61K38/00 , C12Q1/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/14 , C12N2310/141 , C12N2310/53 , C12N2330/10 , C12Q1/6876 , C12Q2600/136 , C12Q2600/178
摘要： In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as "small temporal RNAs" (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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公开(公告)号：EP1407044B1
公开(公告)日：2007-09-19
申请号：EP01985833.1
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie
IPC分类号： C12Q1/68
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
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