公开(公告)号：EP1287123A2

公开(公告)日：2003-03-05

申请号：EP01923084.6

申请日：2001-04-02

申请人： Maxygen, Inc. ,  Novozymes A/S

发明人： NESS, Jon, E. ,  WELCH, Mark ,  GIVER, Lorraine, J. ,  CHERRY, Joel, R. ,  BORCHERT, Torben, V. ,  STEMMER, Willem, P., C. ,  MINSHULL, Jeremy

IPC分类号： C12N9/54 ,  C12N15/57 ,  C07K16/40 ,  G06F17/50

CPC分类号： C12N9/6408 ,  C12N9/54

摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.

公开(公告)号：EP1287123B1

公开(公告)日：2011-03-02

申请号：EP01923084.6

申请日：2001-04-02

申请人： Maxygen, Inc. ,  Novozymes A/S

发明人： NESS, Jon, E. ,  WELCH, Mark ,  GIVER, Lorraine, J. ,  CHERRY, Joel, R. ,  BORCHERT, Torben, V. ,  STEMMER, Willem, P., C. ,  MINSHULL, Jeremy

IPC分类号： C12N9/54 ,  C12N15/57 ,  C07K16/40 ,  G06F17/50

CPC分类号： C12N9/6408 ,  C12N9/54

摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.

公开(公告)号：EP1015575B1

公开(公告)日：2010-05-19

申请号：EP98907912.4

申请日：1998-03-18

申请人： Novozymes A/S

发明人： BORCHERT, Torben, Vedel ,  KRETZSCHMAR, Titus ,  CHERRY, Joel, R.

IPC分类号： C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/1027

摘要： The present invention relates to a new method of shuffling especially heterologous polynucleotide sequences, screening and/or selection of new recombinant proteins resulting therefrom having a desired biological activity, and especially to production and identification of novel proteases exhibiting desired properties. The method comprises the following steps; i) identification of at least one conserved region between the heterologous sequences of interest; ii) generating fragments of each of the heterologous sequences of interest, wherein said fragments comprise the conserved region(s), in a preferred embodiment due to the use of parts of the regions(s) as primers; and iii) shuffling/recombining said fragments using the conserved region(s) as (a) homologous linking point(s).

公开(公告)号：EP0996718B1

公开(公告)日：2008-12-24

申请号：EP98907910.8

申请日：1998-03-18

申请人： Novozymes A/S

发明人： BORCHERT, Torben, Vedel ,  KRETZSCHMAR, Titus ,  CHERRY, Joel, R.

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12N9/00

CPC分类号： C12N15/1093 ,  C12N9/20 ,  C12N9/2417 ,  C12N9/54 ,  C12N15/1027

摘要： A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15 % to more than 80 %, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.

公开(公告)号：EP1015611B1

公开(公告)日：2014-09-03

申请号：EP98910442.7

申请日：1998-03-17

申请人： Novozymes A/S ,  Novozymes, Inc.

发明人： JENSEN, Ejner, Bech ,  CHERRY, Joel, R. ,  ELROD, Susan, L.

IPC分类号： C12N15/64 ,  C12P21/02 ,  C12N1/14 ,  C12R1/69

CPC分类号： C12N9/1029 ,  C12N9/88 ,  C12N15/102 ,  C12N15/64 ,  C12P21/02

公开(公告)号：EP0882123B1

公开(公告)日：2004-09-22

申请号：EP97900947.9

申请日：1997-01-29

申请人： Novozymes A/S

发明人： VON DER OSTEN, Claus ,  CHERRY, Joel, R. ,  BJORNVAD, Mads, Eskelund ,  VIND, Jesper ,  RASMUSSEN, Michael, Dolberg

IPC分类号： C11D3/386 ,  C07K19/00 ,  C12N9/00

CPC分类号： C12N9/0061 ,  C07K2319/00 ,  C11D3/386 ,  C12N9/0065 ,  C12N9/20 ,  C12N9/2417 ,  C12N9/2437 ,  C12Y302/01004