-
公开(公告)号：EP2270235A1
公开(公告)日：2011-01-05
申请号：EP10179400.6
申请日：2005-10-10
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Keunho , Bibette, Jerome
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
摘要翻译： 本发明描述了一种用于分离编码具有期望活性的基因产物的一种或多种遗传元件的方法，包括以下步骤：（a）将遗传元件分隔成微胶囊; 和（b）分选表达具有所需活性的基因产物的遗传元件; 其中至少一个步骤处于微流控制之下。 本发明能够通过本发明方法的重复诱变和迭代应用来体外进化核酸和蛋白质。
-
2.发明公开Compartmentalised combinatorial chemistry by microfluidic control 审中-公开Gegliederte kombinatorische Chemie durch mikrofluidische Steuerung
公开(公告)号：EP2556883A1
公开(公告)日：2013-02-13
申请号：EP12173230.9
申请日：2005-10-12
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Keunho , Bibette, Jerome
CPC分类号： B01F13/0071 , B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0076 , B01J19/0046 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00599 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B01J2219/00853 , B01J2219/0086 , B01J2219/00889 , B01L3/5025 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L2200/0605 , B01L2200/0673 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , B82Y10/00 , B82Y30/00 , C07K1/047 , C40B40/06 , C40B40/10 , C40B50/08 , G01N2035/1034
摘要： The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control. The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.
摘要翻译： 本发明描述了一种合成化合物的方法，包括以下步骤：（a）将两组或多组初级化合物分隔成微胶囊; 使得一部分微胶囊含有两种或更多种化合物; 和（b）通过来自不同组的主要化合物之间的化学反应在微胶囊中形成次级化合物; 其中步骤（a）和（b）中的一个或两个在微流控制下进行; 优选电子微流控制。 本发明还允许鉴定与生物化学体系的靶组分结合或调节靶的活性并且共分隔成微胶囊的化合物。
-
公开(公告)号：EP2270236B1
公开(公告)日：2015-03-04
申请号：EP10179420.4
申请日：2005-10-10
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Keunho , Bibette, Jerome
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
-
公开(公告)号：EP2270235B1
公开(公告)日：2014-10-01
申请号：EP10179400.6
申请日：2005-10-10
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Keunho , Bibette, Jerome
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
-
公开(公告)号：EP2270236A1
公开(公告)日：2011-01-05
申请号：EP10179420.4
申请日：2005-10-10
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Keunho , Bibette, Jerome
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
-
6.发明公开Compartmentalised screening by microfluidic control 有权Unterteilte Untersuchung durch mikrofluidische Steuerung
公开(公告)号：EP2602018A1
公开(公告)日：2013-06-12
申请号：EP12195355.8
申请日：2005-10-12
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Kuenho , Bibette, Jerome
IPC分类号： B01F5/02
CPC分类号： G01N33/5432 , B01F3/0807 , B01F5/0256 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01F2003/0834 , B01F2003/0842 , B01J19/0046 , B01J2219/00576 , B01J2219/00596 , B01J2219/00599 , B01J2219/00657 , B01J2219/00664 , B01J2219/00666 , B01J2219/00702 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12Q1/42 , C12Q2563/159 , C12Q2565/119 , C40B50/08 , G01N15/14 , G01N21/6428 , G01N33/5008 , G01N33/5044 , G01N33/573 , G01N2015/149 , G01N2021/6439 , G01N2333/916 , G01N2500/04 , G01N2500/10
摘要： The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.
摘要翻译： 本发明描述了用于鉴定与生化系统的靶组分结合或调节靶的活性的化合物的方法，包括以下步骤：a）将化合物与靶一起分隔成微胶囊，使得只有一个亚组 在任何一个微胶囊中以多份拷贝表示; 和b）鉴定结合或调节靶标活性的化合物; 其中在微流控制下进行至少一个步骤。 本发明能够筛选可用作药物开发潜力的大分子谱系。
-
公开(公告)号：EP2602018B1
公开(公告)日：2016-04-27
申请号：EP12195355.8
申请日：2005-10-12
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Kuenho , Bibette, Jerome
IPC分类号： B01F5/02
CPC分类号： G01N33/5432 , B01F3/0807 , B01F5/0256 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01F2003/0834 , B01F2003/0842 , B01J19/0046 , B01J2219/00576 , B01J2219/00596 , B01J2219/00599 , B01J2219/00657 , B01J2219/00664 , B01J2219/00666 , B01J2219/00702 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12Q1/42 , C12Q2563/159 , C12Q2565/119 , C40B50/08 , G01N15/14 , G01N21/6428 , G01N33/5008 , G01N33/5044 , G01N33/573 , G01N2015/149 , G01N2021/6439 , G01N2333/916 , G01N2500/04 , G01N2500/10
摘要： The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.
-
公开(公告)号：EP2949761A1
公开(公告)日：2015-12-02
申请号：EP15163413.6
申请日：2005-10-10
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Keunho , Bibette, Jerome
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
摘要翻译： 本发明描述了一种用于分离编码具有所需活性的基因产物的一种或多种遗传元件的方法，包括以下步骤：（a）将遗传元件分隔成微胶囊; 和（b）分选表达具有所需活性的基因产物的遗传元件; 其中至少一个步骤处于微流控制之下。 本发明能够通过本发明方法的重复诱变和迭代应用来体外进化核酸和蛋白质。
-
公开(公告)号：EP2267163B1
公开(公告)日：2015-04-15
申请号：EP10179380.0
申请日：2005-10-10
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Keunho , Bibette, Jerome
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
-
公开(公告)号：EP2267163A1
公开(公告)日：2010-12-29
申请号：EP10179380.0
申请日：2005-10-10
发明人： Griffiths, Andrew , Weitz, David , Link, Darren , Ahn, Keunho , Bibette, Jerome
CPC分类号： B01F3/0807 , B01F5/0646 , B01F5/0647 , B01F5/0655 , B01F13/0062 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/0052 , B01J2219/00545 , B01J2219/00576 , B01J2219/00657 , B01J2219/00722 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C12N15/1058 , C12N15/1075 , C12P21/00 , C12Q1/6874 , G01N15/1459 , G01N15/1484 , G01N2015/149
摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
摘要翻译： 本发明描述了一种用于分离编码具有期望活性的基因产物的一种或多种遗传元件的方法，包括以下步骤：（a）将遗传元件分隔成微胶囊; 和（b）分选表达具有所需活性的基因产物的遗传元件; 其中至少一个步骤处于微流控制之下。 本发明能够通过本发明方法的重复诱变和迭代应用来体外进化核酸和蛋白质。
-
-
-
-
-
-
-
-
-
搜索结果
10 条记录 (耗时 0.043 秒)
