专利检索 ap:("Merck & Co., Inc." OR "University of Kent at Canterbury") AND inv:"MARKUS, Henry, Z." 第 1 页

1.

发明公开
METHOD FOR INCREASING PRODUCTION OF DISULFIDE BONDED RECOMBINANT PROTEINS BY $i(SACCHAROMYCES CEREVISIAE) 失效
标题翻译：法提高重组蛋白的产量二硫键连接-i（酿酒酵母）

公开(公告)号：EP0746611A1

公开(公告)日：1996-12-11

申请号：EP93915201.0

申请日：1993-06-02

申请人： Merck & Co., Inc. , THE UNIVERSITY OF KENT AT CANTERBURY

发明人： TUITE, Michael F. , FREEDMAN, Robert B. , SCHULTZ, Loren, D. , ELLIS, Ronald, W. , MARKUS, Henry, Z. , MONTGOMERY, Donna, L.

IPC分类号： C12N15 , C07K14 , C12N1 , C12N9 , C12P21 , C12R1

CPC分类号： C12N9/90 , C07K14/811 , C07K14/815 , C12N15/81

摘要： Disclosed is a process for increasing the yield of disulfide bonded recombinant proteins produced by yeast, especially recombinant secreted proteins. The enzyme protein disulfide isomerase (PDI) catalyzes the formation of disulfide bonds in secretory and cell-surface proteins. We disclose the construction of recombinant strains of the yeast Saccharomyces cerevisiae which overproduce either human PDI or yeast PDI in a regulated fashion. These strains show greatly increased secretion of disulfide bonded proteins of potential therapeutic significance. These strains have the potential to increase the production of various disulfide bonded proteins.

2.

发明授权
METHOD FOR INCREASING PRODUCTION OF DISULFIDE BONDED RECOMBINANT PROTEINS BY (SACCHAROMYCES CEREVISIAE) 失效
标题翻译：法用于提高重组蛋白的产量二硫键连接（酿酒酵母）

公开(公告)号：EP0746611B1

公开(公告)日：2003-04-16

申请号：EP93915201.3

申请日：1993-06-02

申请人： Merck & Co., Inc. , University of Kent at Canterbury

发明人： TUITE, Michael F. , FREEDMAN, Robert B. , SCHULTZ, Loren, D. , ELLIS, Ronald, W. , MARKUS, Henry, Z. , MONTGOMERY, Donna, L.

IPC分类号： C12N15/12 , C12N15/61 , C12N15/81 , C12N9/90 , C12N1/19 , C07K14/815

CPC分类号： C12N9/90 , C07K14/811 , C07K14/815 , C12N15/81

摘要： Disclosed is a process for increasing the yield of disulfide bonded recombinant proteins produced by yeast, especially recombinant secreted proteins. The enzyme protein disulfide isomerase (PDI) catalyzes the formation of disulfide bonds in secretory and cell-surface proteins. We disclose the construction of recombinant strains of the yeast Saccharomyces cerevisiae which overproduce either human PDI or yeast PDI in a regulated fashion. These strains show greatly increased secretion of disulfide bonded proteins of potential therapeutic significance. These strains have the potential to increase the production of various disulfide bonded proteins.

3.

发明公开
OPTIMIZED EXPRESSION OF HPV 31 L1 IN YEAST 有权
标题翻译： HPV 31 L1的在酵母最佳表达

公开(公告)号：EP1608767A2

公开(公告)日：2005-12-28

申请号：EP04757990.9

申请日：2004-03-19

申请人： Merck & Co. Inc.

发明人： JANSEN, Kathrin, U. , SCHULTZ, Loren, D. , NEEPER, Michael, P. , MARKUS, Henry, Z.

IPC分类号： C12P21/06

CPC分类号： C07K14/005 , A61J1/035 , A61J7/04 , A61K31/66 , A61K31/675 , A61K2039/5258 , C12N7/00 , C12N2710/20022 , C12N2710/20023 , C12N2800/22

摘要： Synthetic DNA molecules encoding the HPV31 L1 protein are provided. Specifically, the present invention provides polynucleotides encoding HPV31 L1 protein, wherein said polynucleotides are free from internal transcription termination signals that are recognized by yeast. Also provided are synthetic polynucleotides encoding HPV31 L1 wherein the polynucleotides have been codon-optimized for high level expression in a yeast cell. The synthetic molecules may be used to produce HPV31 virus-like particles (VLPs), and to produce vaccines and pharmaceutical compositions comprising the HPV31 VLPs. The vaccines of the present invention provide effective immunoprophylaxis against papillomavirus infection through neutralizing antibody and cell-mediated immunity.