-
1.发明公开METHOD FOR PRODUCING PERIPHERAL BLOOD MONOCYTE-DERIVED PLURIPOTENT STEM CELLS 有权PROCESS外周血干细胞的单核细胞来源多一种产生
公开(公告)号：EP2639297A4
公开(公告)日：2014-09-03
申请号：EP11839764
申请日：2011-11-08
发明人： NAKANISHI MAHITO , NISHIMURA KEN , SANO MASAYUKI , OHTAKA MANAMI
CPC分类号： C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2501/65 , C12N2502/1157 , C12N2502/13 , C12N2506/115 , C12N2510/00
摘要： Induced pluripotent stem cells having genetic information identical to that of a patient and yet having nature close to those of ES cells are prepared from human peripheral blood monocytes without leaving those genes in the resultant cells after use for their preparation. Reprogramming genes are loaded on sustained expression-inducing Sendai viral vectors which do not have an activity for integrating foreign genetic information into chromosomes and they are introduced into peripheral blood-derived monocytes to be expressed. Subsequently, the vector genome RNA comprising the reprogramming genes is removed from the cells to establish induced pluripotent stem cells. The above-described Sendai viral vector is capable of extremely simple and efficient preparation of induced pluripotent stem cells having genetic information identical to that of an individual who supplied the differentiated cell and yet this vector is safe presenting only a low risk of tumorigenesis. By supplying useful induced pluripotent stem cells, this Sendai viral vector can serve as a powerful tool in various applications including safe and efficient performance of cell replacement therapy.
-
2.发明公开VECTOR MATERIAL FOR CREATING PLURIPOTENT STEM CELLS, AND PLURIPOTENT STEM CELL CREATION METHOD USING SAID VECTOR MATERIAL 有权矢量素材于产生多能干细胞的生成方法干细胞使用该载体材料的多潜能
公开(公告)号：EP2434012A4
公开(公告)日：2013-11-06
申请号：EP10777759
申请日：2010-05-18
发明人： NAKANISHI MAHITO , NISHIMURA KEN , OHTAKA MANAMI , SANO MASAYUKI
IPC分类号： C12N15/09 , C12N5/071 , C12N5/10 , C12N7/06 , C12N15/11 , C12N15/113 , C12N15/63 , C12N15/86 , C12N15/877
CPC分类号： C12N15/86 , C12N5/0696 , C12N15/111 , C12N15/1131 , C12N15/8775 , C12N15/8776 , C12N2310/14 , C12N2330/50 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2506/1307 , C12N2510/00 , C12N2760/18841 , C12N2760/18843 , C12N2760/18862
摘要： Cell reprogramming genes are loaded on a sustained expression-type Sendai virus vector free of an activity of incorporating exogenous genetic information into a chromosome. The genes are transfected into a normal differentiated cell via the vector, and expressed. Then, a vector genomic RNA including the genes is removed from the cell to establish an induced pluripotent stem cell. The Sendai virus vector makes it possible to generate an induced pluripotent stem cell having genomic information identical to that of a donor individual of the differentiated cell, in a significantly simple and efficient manner and in a safe manner with less risk of tumorigenic transformation. Thus, the Sendai virus vector can serve as an important tool for performing cell replacement therapies in a safe and efficient manner though the provision of useful induced pluripotent stem cells.
-
搜索结果
2 条记录 (耗时 0.008 秒)
