公开(公告)号：EP2968392A4

公开(公告)日：2016-08-03

申请号：EP14762788

申请日：2014-03-14

申请人： ELIXIRGEN LLC

发明人： KO MINORU S H

IPC分类号： A61K31/7088 ,  A61K48/00 ,  C12N15/09 ,  C12N15/11

CPC分类号： A61K38/1709 ,  A61K9/51 ,  A61K31/7088 ,  A61K35/28 ,  A61K35/33 ,  A61K35/545 ,  A61K48/00 ,  A61K48/005 ,  C12N5/0606 ,  C12N5/0609 ,  C12N5/0696 ,  C12N15/113 ,  C12N15/85 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/31 ,  C12N2501/998 ,  C12N2510/04 ,  C12N2760/18841 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158

摘要： The present disclosure relates to methods for increasing telomere length in one or more human cells and/or increasing genome stability of one or more human cells, for example by contacting one or more human cells with an agent that increases expression of Zscan4 in the one or more human cells. Methods of treating a subject in need of telomere lengthening, treating a disease or condition associated with a genomic and/or chromosome abnormality, of rejuvenating one or more human cells, of rejuvenating tissues or organs, and of rejuvenating a subject in need thereof, for example by contacting one or more human cells in the subject with an agent that increases expression of Zscan4, or by administering to a subject in need thereof, an agent that increases expression of Zscan4 are also provided.

公开(公告)号：EP2434012A1

公开(公告)日：2012-03-28

申请号：EP10777759.1

申请日：2010-05-18

申请人： National Institute of Advanced Industrial Science and Technology

发明人： NAKANISHI, Mahito ,  NISHIMURA, Ken ,  OHTAKA, Manami ,  SANO, Masayuki

IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N7/06 ,  C12N15/113 ,  C12N15/63

CPC分类号： C12N15/86 ,  C12N5/0696 ,  C12N15/111 ,  C12N15/1131 ,  C12N15/8775 ,  C12N15/8776 ,  C12N2310/14 ,  C12N2330/50 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2760/18841 ,  C12N2760/18843 ,  C12N2760/18862

摘要： Cell reprogramming genes are loaded on a sustained expression-type Sendai virus vector free of an activity of incorporating exogenous genetic information into a chromosome. The genes are transfected into a normal differentiated cell via the vector, and expressed. Then, a vector genomic RNA including the genes is removed from the cell to establish an induced pluripotent stem cell. The Sendai virus vector makes it possible to generate an induced pluripotent stem cell having genomic information identical to that of a donor individual of the differentiated cell, in a significantly simple and efficient manner and in a safe manner with less risk of tumorigenic transformation. Thus, the Sendai virus vector can serve as an important tool for performing cell replacement therapies in a safe and efficient manner though the provision of useful induced pluripotent stem cells.

摘要翻译： 细胞重编程基因被装载在自由掺入外源遗传信息到染色体的活性的持续表达型仙台病毒载体。 所述基因通过所述载体转染到正常分化的细胞，和过表达。 然后，载体基因组RNA包含的基因从细胞除去，以建立在诱导多能干细胞。 仙台病毒载体能够产生的诱导多能干细胞具有致瘤转变的风险更小的基因组信息，等同于捐赠者做了分化细胞的个体，在一个简单而有效的方式显着且安全的方式。 因此，仙台病毒载体可以作为在以安全和有效的方式进行细胞替代疗法的重要工具虽然提供有用的诱导多能干细胞。

公开(公告)号：EP2434012B1

公开(公告)日：2017-12-13

申请号：EP10777759.1

申请日：2010-05-18

申请人： National Institute of Advanced Industrial Science and Technology

发明人： NAKANISHI, Mahito ,  NISHIMURA, Ken ,  OHTAKA, Manami ,  SANO, Masayuki

IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N7/06 ,  C12N15/113 ,  C12N15/63 ,  C12N5/074 ,  C12N15/11 ,  C12N15/86 ,  C12N15/877 ,  C12N5/071

CPC分类号： C12N15/86 ,  C12N5/0696 ,  C12N15/111 ,  C12N15/1131 ,  C12N15/8775 ,  C12N15/8776 ,  C12N2310/14 ,  C12N2330/50 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2760/18841 ,  C12N2760/18843 ,  C12N2760/18862

摘要： Cell reprogramming genes are loaded on a sustained expression-type Sendai virus vector free of an activity of incorporating exogenous genetic information into a chromosome. The genes are transfected into a normal differentiated cell via the vector, and expressed. Then, a vector genomic RNA including the genes is removed from the cell to establish an induced pluripotent stem cell. The Sendai virus vector makes it possible to generate an induced pluripotent stem cell having genomic information identical to that of a donor individual of the differentiated cell, in a significantly simple and efficient manner and in a safe manner with less risk of tumorigenic transformation. Thus, the Sendai virus vector can serve as an important tool for performing cell replacement therapies in a safe and efficient manner though the provision of useful induced pluripotent stem cells.

公开(公告)号：EP2434012A4

公开(公告)日：2013-11-06

申请号：EP10777759

申请日：2010-05-18

申请人： NAT INST OF ADVANCED IND SCIEN

发明人： NAKANISHI MAHITO ,  NISHIMURA KEN ,  OHTAKA MANAMI ,  SANO MASAYUKI

IPC分类号： C12N15/09 ,  C12N5/071 ,  C12N5/10 ,  C12N7/06 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/86 ,  C12N15/877

CPC分类号： C12N15/86 ,  C12N5/0696 ,  C12N15/111 ,  C12N15/1131 ,  C12N15/8775 ,  C12N15/8776 ,  C12N2310/14 ,  C12N2330/50 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2760/18841 ,  C12N2760/18843 ,  C12N2760/18862

摘要： Cell reprogramming genes are loaded on a sustained expression-type Sendai virus vector free of an activity of incorporating exogenous genetic information into a chromosome. The genes are transfected into a normal differentiated cell via the vector, and expressed. Then, a vector genomic RNA including the genes is removed from the cell to establish an induced pluripotent stem cell. The Sendai virus vector makes it possible to generate an induced pluripotent stem cell having genomic information identical to that of a donor individual of the differentiated cell, in a significantly simple and efficient manner and in a safe manner with less risk of tumorigenic transformation. Thus, the Sendai virus vector can serve as an important tool for performing cell replacement therapies in a safe and efficient manner though the provision of useful induced pluripotent stem cells.