公开(公告)号：EP2428578A4

公开(公告)日：2013-09-11

申请号：EP10772125

申请日：2010-05-10

申请人： NAT UNIV CORP KAGOSHIMA ,  EZAKI GLICO CO

发明人： KADOKAWA JUNICHI ,  TAKAHA TAKESHI ,  KUBO AKIKO ,  YANASE MICHIYO ,  HOSOYA KAYO

IPC分类号： C12P19/04 ,  A61K47/36 ,  C08B37/00 ,  C12N9/12 ,  C12N15/09

CPC分类号： A61K47/36 ,  A61K49/0002 ,  C08B37/0009 ,  C12N9/1051 ,  C12P19/04 ,  C12Y204/01001

公开(公告)号：EP2636748A4

公开(公告)日：2014-04-02

申请号：EP11837764

申请日：2011-11-07

申请人： EZAKI GLICO CO

发明人： TAKAHA TAKESHI ,  KUBO AKIKO ,  YANASE MICHIYO

IPC分类号： C12P19/26 ,  A61K47/36 ,  A61K47/48 ,  A61K49/00 ,  C08B31/00 ,  C08B35/00 ,  C08B37/00 ,  C08B37/16 ,  C12N15/87

CPC分类号： A61K47/36 ,  A61K9/5161 ,  A61K47/59 ,  A61K47/6455 ,  A61K49/0054 ,  C08B31/00 ,  C08B35/00 ,  C08B37/0009 ,  C08B37/0012 ,  C08B37/0063 ,  C12N15/87 ,  C12P19/26

摘要： An object of the present invention is to provide a glucosamine-containing glucan, a modified product and a conjugate thereof. The glucosamine-containing glucan of the present invention is a glucosamine-containing glucan wherein the glucan has a plurality of non-reducing ends and at least one glucosamine residue is bound via an ±-1,4-bond to each of two or more non-reducing ends of the branched ±-1,4-glucan, but no glucosamine residue is present at a position other than the non-reducing ends of the branched ±-1,4-glucan, wherein the degree of polymerization of the glucan is 15 or more and 4 x 10 5 or less. Theglucosamine-containingglucan of the present invention can be provided by allowing an ±-glucan phosphorylase to act on an aqueous solution comprising a branched ±-1,4-glucan and glucosamine-1-phosphate.

公开(公告)号：EP2636749A4

公开(公告)日：2014-04-02

申请号：EP11837765

申请日：2011-11-07

申请人： EZAKI GLICO CO

发明人： TAKAHA TAKESHI ,  YANASE MICHIYO ,  KUBO AKIKO ,  SAMBE HARUYO ,  KAKUTANI RYO

IPC分类号： C12P19/26 ,  A61K47/36 ,  C08B37/00

CPC分类号： C08B37/0009 ,  A61K9/5161 ,  A61K47/36 ,  A61K47/61 ,  A61K2039/55583 ,  C08B37/0063 ,  C12P19/04 ,  C12P19/28 ,  C12Y204/01001

公开(公告)号：EP1661985A4

公开(公告)日：2006-12-20

申请号：EP04772489

申请日：2004-08-31

申请人： EZAKI GLICO CO

发明人： FUJII KAZUTOSHI ,  IIBOSHI MASAE ,  YANASE MICHIYO ,  TAKATA HIROKI ,  TAKAHA TAKESHI ,  KURIKI TAKASHI

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12P19/02 ,  C12P19/04

CPC分类号： C12N9/1051

摘要： It is intended to provide a heat-stable sucrose phosphorylase (SP) obtained by modifying natural SP and a method of preparing this heat-stable SP. Namely, a heat-stable SP which has an amino acid residue differing from the one in natural sucrose phosphorylase at least one position selected from the group consisting of: the positions corresponding respectively to the 14-, 29- and 44-positions in the motif sequence 1; the positions corresponding respectively to the 7-, 19-, 23- and 34-positions in the motif sequence 2; and the position corresponding to the 19-position in the motif sequence 3, and, after heating in a 20 mM Tris buffer solution (pH 7.0) at 55°C for 20 minutes, shows an enzymatic activity amounting to 20% or more of the enzymatic activity of the unheated heat-stable SP at 37°C.

摘要翻译： 提供了通过改善天然SP获得的具有改善的热稳定性的蔗糖磷酸化酶（SP）和具有改进的热稳定性的制备SP的方法。 具有改善的热稳定性的SP具有与天然蔗糖磷酸化酶不同的氨基酸残基，所述氨基酸残基在选自对应于位置14的位置，对应于位置29的位置和对应于 基序序列1中的位置44; 对应于位置7的位置，对应于位置19的位置，对应于位置23的位置和对应于图案序列2中的位置34的位置; 和与基序序列3中的位置19相对应的位置，并且其中在55℃下加热在20mM Tris缓冲液（pH7.0）中具有改善的热稳定性的SP后，在37℃下具有改善的热稳定性的SP的酶活性为20 分钟，在加热前在37℃下具有改善的热稳定性的SP的酶活性的20％或更多。

公开(公告)号：EP1390520A4

公开(公告)日：2004-09-08

申请号：EP02774073

申请日：2002-05-27

申请人： EZAKI GLICO CO

发明人： FUJII KAZUTOSHI ,  TERADA YOSHINOBU ,  YANASE MICHIYO ,  ODAN KOJI ,  TAKATA HIROKI ,  TAKAHA TAKESHI ,  KURIKI TAKASHI ,  OKADA SHIGETAKA

IPC分类号： C12P19/04 ,  C08B30/20 ,  C08B33/00 ,  C08B37/00 ,  C12P19/18 ,  C12R1/46 ,  C08B15/00 ,  C08B30/00

CPC分类号： C08B37/0009 ,  C08B30/20 ,  C08B33/00 ,  C12P19/18

摘要： A first method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The maximum value of the sucrose-phosphate ratio of the reaction solution from the start of the reaction to the end of the reaction is no more than about 17. A second method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The reaction is conducted at a temperature of about 40 C to about 70 C.