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    Novel bacteriophage and method for breeding thereof
    1.
    发明公开
    Novel bacteriophage and method for breeding thereof 失效
    Bakteriophage和Verfahren zurZüchtung。

    公开(公告)号：EP0095934A2

    公开(公告)日：1983-12-07

    申请号：EP83303125.5

    申请日：1983-06-01

    申请人： NODA INSTITUTE FOR SCIENTIFIC RESEARCH

    发明人： Nakana, Eiichi ,  Masuda, Tsutomu ,  Koyama, Yasuji ,  Kitao, Satoshi

    IPC分类号： C12N15/11 ,  C12N15/10 ,  C12N15/73 ,  C12N7/00 ,  C12N1/20

    CPC分类号： C12N15/70 ,  C12N15/73

    摘要： The DNA of a novel bacteriophage is characterized in that (1) in the upstream portion from the late promoter necessary for the transcription of genetic information for the biosynthesis of coat proteins up to the cohesive end, there is no endonuclease cleavage site or there are endonuclease cleavage sites permissible in number for the reconstruction of a DNA molecule when the DNA is cleaved with endonuclease into fragments and then these fragments are rejoined with DNA ligase, and (2) in the downstream portion from the late promoter down to the cohesive end, there is one or more endonuclease cleavage sites.

    摘要翻译： 新型噬菌体的DNA的特征在于，（1）在后期启动子的上游部分，用于转录用于生物合成外壳蛋白直到粘性末端的遗传信息，没有内切核酸酶切割位点或有内切核酸酶 当DNA用内切核酸酶切割成片段时，重组DNA分子的数目允许的切割位点，然后这些片段用DNA连接酶重新连接，和（2）在后期启动子的下游部分到粘性末端，那里 是一个或多个内切核酸酶切割位点。