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    MAMMALIAN EXPRESSION VECTOR
    3.
    发明公开
    MAMMALIAN EXPRESSION VECTOR 审中-公开

    公开(公告)号：EP3680337A1

    公开(公告)日：2020-07-15

    申请号：EP20153831.1

    申请日：2008-12-18

    申请人： Novartis AG

    发明人： KNOPF, Hans-Peter ,  WILMS, Burkhard

    IPC分类号： C12N15/79

    摘要： The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising
    (a) at least one expression cassette (POI) for expressing a polypeptide of interest;
    (b) an expression cassette (MSM) comprising a mammalian selectable marker gene;
    (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene;
    wherein the expression cassette (POI) is flanked 5' by the expression cassette (MASM), the expression cassette (MSM) is located 3' from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5' to 3' orientation.
    Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.

    MAMMALIAN EXPRESSION VECTOR
    4.
    发明公开
    MAMMALIAN EXPRESSION VECTOR 有权
    哺乳动物表达载体

    公开(公告)号：EP2227546A1

    公开(公告)日：2010-09-15

    申请号：EP08865499.1

    申请日：2008-12-18

    申请人： Novartis AG

    发明人： KNOPF, Hans-Peter ,  WILMS, Burkhard

    IPC分类号： C12N15/79

    CPC分类号： C12N15/85

    摘要： The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising (a) at least one expression cassette (POI) for expressing a polypeptide of interest; (b) an expression cassette (MSM) comprising a mammalian selectable marker gene; (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene; wherein the expression cassette (POI) is flanked 5' by the expression cassette (MASM), the expression cassette (MSM) is located 3' from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5' to 3' orientation. Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.

    METHOD OF SCREENING CELL CLONES
    6.
    发明公开
    METHOD OF SCREENING CELL CLONES 审中-公开
    一种筛选细胞克隆

    公开(公告)号：EP2970956A1

    公开(公告)日：2016-01-20

    申请号：EP14714386.1

    申请日：2014-03-10

    申请人： Novartis AG

    发明人： NOMMAY, Audrey ,  WILMS, Burkhard

    IPC分类号： C12N15/10 ,  G01N33/569

    CPC分类号： C12N15/1079 ,  G01N15/14 ,  G01N33/56966 ,  G01N2015/1006 ,  G01N2015/1402

    摘要： A method of screening cell clones expressing a high yield of a polypeptide of interest is provided. The method employs the consecutive use of fluorescence activated cell sorting followed by colony picking based selection of cell clones with high expression rates and high proliferation rates. Furthermore, the invention pertains to a method of producing a polypeptide of interest using cells obtained by the described screening method.

    OPTIMIZED EXPRESSION CASSETTE FOR EXPRESSING A POLYPEPTIDE WITH HIGH YIELD
    8.
    发明授权
    OPTIMIZED EXPRESSION CASSETTE FOR EXPRESSING A POLYPEPTIDE WITH HIGH YIELD 有权
    最佳表达克司特酸性表达蛋白多糖MIT HOHER AUSBEUTE

    公开(公告)号：EP2922962B1

    公开(公告)日：2016-12-21

    申请号：EP13792030.2

    申请日：2013-11-18

    申请人： Novartis AG

    发明人： DRAGIC, Zorica ,  SCHMITZ, Rita ,  WILMS, Burkhard ,  GEISSE, Sabine

    IPC分类号： C12N15/85 ,  C12N5/10

    CPC分类号： C12N15/85 ,  C07K16/00 ,  C12N2830/00 ,  C12N2830/30

    摘要： The present invention is based on the finding that the combination of a specific 5′UTR polynucleotide sequence (see SEQ ID NO 1) and the hCD33 secretory leader sequence in an expression cassette for expressing a polypeptide of interest results in a surprisingly better expression level of the polypeptide of interest compared to prior art expression cassettes. Based on this finding, the present invention inter alia provides novel expression cassettes, expression vectors and methods for producing a polypeptide of interest with high yield.

    摘要翻译： 本发明是基于以下发现：用于表达目的多肽的表达盒中特异性5'UTR多核苷酸序列（见SEQ ID NO 1）和hCD33分泌前导序列的组合导致惊人的更好的表达水平 感兴趣的多肽与现有技术的表达盒相比。 基于这一发现，本发明尤其提供了新的表达盒，表达载体和用于以高产率产生目的多肽的方法。

    MAMMALIAN EXPRESSION VECTOR
    9.
    发明公开
    MAMMALIAN EXPRESSION VECTOR 审中-公开
    加拿大梭鲈，EXPRESSIONSVEKTOR

    公开(公告)号：EP3075858A1

    公开(公告)日：2016-10-05

    申请号：EP16168059.0

    申请日：2008-12-18

    申请人： NOVARTIS AG

    发明人： KNOPF, Hans-Peter ,  WILMS, Burkhard

    IPC分类号： C12N15/79

    摘要： The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising
    (a) at least one expression cassette (POI) for expressing a polypeptide of interest;
    (b) an expression cassette (MSM) comprising a mammalian selectable marker gene;
    (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene;
    wherein the expression cassette (POI) is flanked 5' by the expression cassette (MASM), the expression cassette (MSM) is located 3' from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5' to 3' orientation.
    Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.

    摘要翻译： 本发明提供用于在哺乳动物细胞中表达至少一种目的多肽的载体核酸，其包含（a）用于表达目的多肽的至少一种表达盒（POI）; （b）包含哺乳动物选择标记基因的表达盒（MSM）; （c）包含哺乳动物可扩增的可选择标记基因的表达盒（MASM）; 其中表达盒（POI）通过表达盒（MASM）在5'侧翼，表达盒（MSM）位于表达盒（POI）3'，其中表达盒（MASM），（POI）和（ MSM）以相同的5'至3'方向排列。 还提供了包含所述载体的宿主细胞和使用各自宿主细胞产生多肽的方法。

    CELL SURFACE DISPLAY OF POLYPEPTIDE ISOFORMS BY STOP CODON READTHROUGH
    10.
    发明公开
    CELL SURFACE DISPLAY OF POLYPEPTIDE ISOFORMS BY STOP CODON READTHROUGH 有权
    演示BY POLYPEPTIDISOFORMEN停止细胞表面密码子通读

    公开(公告)号：EP2329020A1

    公开(公告)日：2011-06-08

    申请号：EP09778177.7

    申请日：2009-08-28

    申请人： Novartis AG

    发明人： JOSTOCK, Thomas ,  KNOPF, Hans-Peter ,  WILMS, Burkhard ,  NOMMAY, Audrey Josiane

    IPC分类号： C12N15/10 ,  C12N15/62

    CPC分类号： C12N15/1037 ,  C07K2319/035

    摘要： The present invention inter alia pertains to a method for generating or selecting a eukaryotic host cell expressing a desired level of a polypeptide of interest, comprising: a) providing a plurality of eukaryotic host cells comprising a heterologous nucleic acid comprising at least one cassette (Cas-POI) comprising at least a first polynucleotide (Pn-POI) encoding the polypeptide of interest, at least one stop codon down-stream of the first polynucleotide, and a second polynucleotide downstream of the stop codon encoding an immunoglobulin transmembrane anchor or a functional variant thereof; b) cultivating the eukaryotic host cells to allow expression of the polypeptide of interest such that at least a portion of the polypeptide of interest is expressed as a fusion polypeptide comprising the immunoglobulin transmembrane anchor or a functional variant thereof, wherein said fusion polypeptide is being displayed on the surface of said host cell; c) selecting at least one eukaryotic host cell based upon the presence or amount of the fusion polypeptide displayed on the cell surface. Also provided are host cells, comprising respectively designed heterologous nucleic acids and methods for producing a polypeptide using respective host cells.