利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

3 条记录 (耗时 0.016 秒)

    CELL ISOLATION METHOD AND CELL TRAPPING FILTER
    1.
    发明公开
    CELL ISOLATION METHOD AND CELL TRAPPING FILTER 审中-公开
    细胞分离方法和细胞捕获过滤器

    公开(公告)号：EP3214166A1

    公开(公告)日：2017-09-06

    申请号：EP15853920.5

    申请日：2015-10-29

    申请人： National University Corporation Tokyo University of Agriculture and Technology ,  Hitachi Chemical Co., Ltd.

    发明人： YOSHINO, Tomoko ,  TANAKA, Tsuyoshi ,  MATSUNAGA, Tadashi ,  NEGISHI, Ryo ,  KANBARA, Hisashige ,  NAKAMURA, Seita

    IPC分类号： C12N1/02 ,  C12M1/00 ,  C12M1/12 ,  C12M3/06

    CPC分类号： C12M47/04 ,  C12M1/12 ,  C12M1/28 ,  C12M3/06 ,  C12M33/00 ,  C12M33/14 ,  C12M47/02 ,  C12N1/02 ,  G01N1/36 ,  G01N1/4077 ,  G01N33/5091 ,  G01N33/574 ,  G01N2001/4088

    摘要： A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.

    摘要翻译： 细胞分离方法包括：细胞捕获步骤，使测试液体通过在厚度方向上具有多个通孔的细胞捕获过滤器，由此将包含在测试液体中的分离靶细胞捕获在 细胞捕获过滤器; 在细胞捕获步骤中将刺激响应性水凝胶引入其上已经捕获了细胞的细胞捕获过滤器的一个表面上，从而将细胞包埋在刺激响应性水凝胶中的凝胶包埋步骤; 凝胶硬化步骤，对包埋细胞的刺激响应性水凝胶施加刺激，从而使刺激响应性水凝胶硬化; 以及将在凝胶硬化步骤中硬化的刺激响应性水凝胶从细胞捕获过滤器分离的分离步骤。

    MICROFLUIDIC DEVICE FOR TRAPPING SINGLE CELL
    2.
    发明授权
    MICROFLUIDIC DEVICE FOR TRAPPING SINGLE CELL 有权
    用于捕获单细胞的微流体装置

    公开(公告)号：EP2175014B1

    公开(公告)日：2017-08-30

    申请号：EP08790322.5

    申请日：2008-07-31

    申请人： National University Corporation Tokyo University of Agriculture and Technology

    发明人： MATSUNAGA, Tadashi ,  ARAKAKI, Atsushi ,  TAGUCHI, Tomoyuki ,  HOSOKAWA, Masahito

    IPC分类号： C12M1/00 ,  C12M1/26 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/53 ,  G01N37/00

    CPC分类号： G01N15/1484 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/049 ,  C12Q1/6841 ,  G01N15/1475 ,  C12Q2565/629

    摘要： It is to provide a microfluidic device which separates and captures with high efficiency a large amount of cells in a sample at one-cell level without damaging the cells by utilizing a microfabrication technology. The device sequentially comprises: an upper substrate consisting of a plastic plate provided with a groove for forming a microchannel communicating a sample-supply opening and a sample-discharge opening; a plastic upper flat plate forming the microchannel by cooperating with the upper substrate, the plate being provided with an aperture for a micromesh; a holding plate holding a plastic micromesh having a plurality of micro through-pores for capturing cells; a plastic lower flat plate provided with an aperture for suctioning; and a lower substrate consisting of a plastic flat plate provided with a groove for forming a suction channel, wherein the micro through-pores for capturing cells of the micromesh have an inverted mortar-like shape or a cylindrical shape, and wherein the upper flat plate is constituted by a softer plastic as compared to the upper substrate.

    摘要翻译： 提供一种微流体装置，其通过利用微细加工技术在单细胞水平上高效分离和捕获样品中的大量细胞而不损坏细胞。 所述装置依次包括：由塑料板构成的上基板，所述塑料板设置有用于形成连通样品供应开口和样品排出口的微通道的槽; 通过与上基板配合而形成微通道的塑料上平板，所述板设置有微网孔; 保持具有用于捕获细胞的多个微通孔的塑料微网格的保持板; 设有用于抽吸的孔的塑料下平板; 以及由设有用于形成抽吸通道的凹槽的塑料平板组成的下基板，其中用于捕获微网孔的微通孔具有倒置的砂浆状或圆柱形形状，并且其中上平板 与上基板相比由较软的塑料构成。