公开(公告)号：EP3800250A1

公开(公告)日：2021-04-07

申请号：EP19870253.2

申请日：2019-10-10

Applicant: National University Corporation Tottori University ,  Trans Chromosomics, Inc.

Inventor： KAZUKI Yasuhiro ,  UNO Narumi ,  OSHIMURA Mitsuo

IPC: C12N15/06 ,  C12N5/071 ,  C12N5/22 ,  C12N15/07

Abstract: This application provides a method for preparing human cell-derived microcells from human cells comprising DNA of interest, comprising: a step of culturing human cells in a medium containing at least one micronucleus inducer selected from the group consisting of microtubule polymerization inhibitors (excepting colcemid), microtubule depolymerization inhibitors, and spindle checkpoint inhibitors, thereby to produce human cell-derived microcells; and a step of collecting human cell-derived microcells comprising the DNA of interest, and a method for preparing human cells comprising DNA of interest by use of the method, which can be also applied to non-human animal cells.

公开(公告)号：EP4389902A1

公开(公告)日：2024-06-26

申请号：EP22892931.1

申请日：2022-11-15

Applicant: National University Corporation Tottori University

Inventor： KAZUKI Yasuhiro ,  UNO Narumi ,  TOMIZUKA Kazuma

IPC: C12N15/85 ,  C12N5/10

CPC classification number: C12N15/85 ,  C12N5/10

Abstract: Provided is a method for producing a human artificial chromosome vector with high safety by using a human cell. The method for producing a human cell comprising a human artificial chromosome vector comprises: substantially eliminating endogenous genes of long-arm and short-arm in a disomic human cell containing a pair of the homologous human chromosomes or a trisomic human cell containing trisomy of the homologous human chromosomes, from one of two chromosomes of the disomy or one or two of three chromosomes of the trisomy, thereby producing a cell population containing a human cell containing a human artificial chromosome vector comprising long-arm moiety and short-arm moiety substantially containing no endogenous gene, a human centromere, and a telomere; and collecting the human cell containing the human artificial chromosome vector from the cell population. As well as, the human cell comprises the human artificial chromosome vector.

公开(公告)号：EP4389895A1

公开(公告)日：2024-06-26

申请号：EP22895642.1

申请日：2022-11-16

Applicant: National University Corporation Tottori University

Inventor： KAZUKI Yasuhiro ,  TOMIZUKA Kazuma ,  UNO Narumi

IPC: C12N15/13 ,  A01K67/027 ,  C07K16/00 ,  C07K16/06 ,  C12N5/0735 ,  C12N5/10 ,  C12N15/06 ,  C12N15/09 ,  C12N15/10 ,  C12N15/85 ,  C12N15/90 ,  C12P21/08

CPC classification number: C07K16/00 ,  C07K16/06 ,  C12N5/06 ,  C12N5/10 ,  C12N5/16 ,  C12N15/09 ,  C12N15/10 ,  C12N15/85 ,  C12N15/90 ,  A01K67/027

Abstract: Provided is an approach for achieving the expansion of a human antibody repertoire in a human antibody-producing non-human animal. Provided is a mammalian artificial chromosome vector comprising a human immunoglobulin heavy chain locus in which a D-region of a human immunoglobulin heavy chain is modified, and a human immunoglobulin light chain locus, a mammalian cell or a non-human animal comprising this mammalian artificial chromosome vector, and a method for substituting a D-region of a human immunoglobulin heavy chain with a modified sequence thereof in the production of this artificial chromosome vector.

公开(公告)号：EP3800246A1

公开(公告)日：2021-04-07

申请号：EP19872026.0

申请日：2019-10-10

Applicant: National University Corporation Tottori University ,  Trans Chromosomics, Inc.

Inventor： KAZUKI Yasuhiro ,  UNO Narumi ,  OSHIMURA Mitsuo

IPC: C12N5/10 ,  C12N15/07

Abstract: The present application provides: a method for producing human induced pluripotent stem (iPS) cells comprising an exogenous chromosome having a DNA of interest using the MMCT method; and a method for expressing the exogenous gene in the human iPS cells prepared by the method for producing human iPS cells, or in undifferentiated or differentiated cells derived from the human iPS cells induced to differentiate from the human iPS cells.