公开(公告)号：EP3870703A1

公开(公告)日：2021-09-01

申请号：EP19804901.7

申请日：2019-10-21

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services ,  New York University

发明人： FRANCHINI, Genoveffa ,  CARDOZO, Timothy ,  BECERRA-FLORES, Manuel ,  SILVA DE CASTRO, Isabela ,  GORINI, Giacomo ,  BISSA, Massimiliano

IPC分类号： C12N15/10

公开(公告)号：EP3230457A1

公开(公告)日：2017-10-18

申请号：EP15867414.3

申请日：2015-12-09

申请人： New York University

发明人： ICHTCHENKO, Konstantin ,  VAZQUEZ-CINTRON, Edwin ,  BANK, Philip, A. ,  CARDOZO, Timothy

IPC分类号： C12N15/63 ,  C12N15/70

CPC分类号： C07K14/33 ,  A61K2039/505 ,  C07K16/1282 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/41 ,  C07K2319/50

摘要： The present invention is directed to a fusion protein comprising a light chain region of a Clostridial neurotoxin and a heavy chain region of a Clostridial neurotoxin, where the light and heavy chain regions are linked by a disulfide bond. The fusion protein also has a single chain antibody positioned upstream of the light chain region, where the single chain antibody possesses antigen-binding activity. Also disclosed are therapeutic agents, treatment methods, propeptide fusions, isolated nucleic acid molecules, expression systems, host cells, and methods of expressing fusion proteins.

摘要翻译： 本发明涉及包含梭菌神经毒素的轻链区和梭菌神经毒素的重链区的融合蛋白，其中轻链和重链区通过二硫键连接。 融合蛋白还具有位于轻链区上游的单链抗体，其中单链抗体具有抗原结合活性。 还公开了治疗剂，治疗方法，前肽融合物，分离的核酸分子，表达系统，宿主细胞和表达融合蛋白的方法。