公开(公告)号：EP3898985A1

公开(公告)日：2021-10-27

申请号：EP19817783.4

申请日：2019-12-17

申请人： Novozymes A/S

发明人： BAERENDS, Richard, Jan, Steven ,  OLSEN, Carsten, Lillelund ,  ARNAU, Josè

IPC分类号： C12N15/80 ,  C12N15/62

公开(公告)号：EP3327113A2

公开(公告)日：2018-05-30

申请号：EP17200169.5

申请日：2013-05-29

申请人： Novozymes A/S

发明人： OLSEN, Carsten, Lillelund

IPC分类号： C12N1/16 ,  C12N15/81

CPC分类号： C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/88 ,  C12N15/67 ,  C12N15/902 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y107/01004 ,  C12Y402/01011

摘要： The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5' untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.

公开(公告)号：EP2855659A1

公开(公告)日：2015-04-08

申请号：EP13726188.9

申请日：2013-05-29

申请人： Novozymes A/S

发明人： OLSEN, Carsten, Lillelund

IPC分类号： C12N1/16 ,  C12N15/81

CPC分类号： C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/88 ,  C12N15/67 ,  C12N15/902 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y107/01004 ,  C12Y402/01011

摘要： The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5' untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.

摘要翻译： 本发明涉及构建重组真菌宿主细胞的方法，所述重组真菌宿主细胞包含整合在其基因组中的多核苷酸构建体的一个或多个拷贝，所述方法包括用包含编码可选择标记的第一多核苷酸的整合型多核苷酸构建体转化真菌宿主细胞，其中 第一多核苷酸，其5'非翻译区和/或与其可操作地连接的核糖开关包含在其分支位点和其受体位点之间具有5个核苷酸或更少的剪接体内含子; 和编码目的多肽的第二个多核苷酸; 以及合适的多核苷酸构建体，得到的真菌宿主细胞和制造方法。

公开(公告)号：EP3327113A3

公开(公告)日：2018-08-08

申请号：EP17200169.5

申请日：2013-05-29

申请人： Novozymes A/S

发明人： OLSEN, Carsten, Lillelund

IPC分类号： C12N1/16 ,  C12N15/81

CPC分类号： C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/88 ,  C12N15/67 ,  C12N15/902 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y107/01004 ,  C12Y402/01011

摘要： The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5' untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.

公开(公告)号：EP3077514A1

公开(公告)日：2016-10-12

申请号：EP14806626.9

申请日：2014-12-03

申请人： Novozymes A/S

发明人： HANSEN, Bjarne G. ,  OLSEN, Carsten, Lillelund ,  VIND, Jesper

IPC分类号： C12N15/65 ,  C12N15/80 ,  C12N15/90

CPC分类号： C12N15/902 ,  C12N15/80

摘要： The present invention relates to a method for site-specific chromosomal integration of a gene library in a filamentous fungal host cell and a polynucleotide construct suitable for this purpose.

摘要翻译： 本发明涉及丝状真菌宿主细胞中基因文库的位点特异性染色体整合方法和适用于该目的的多核苷酸构建体。