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62 条记录 (耗时 0.017 秒)

    NITROREDUCTASE BIOSENSORS FOR DETECTING NITRO-COMPOUNDS
    4.
    发明公开
    NITROREDUCTASE BIOSENSORS FOR DETECTING NITRO-COMPOUNDS 有权
    硝基还原酶生物传感器的硝基化合物检测

    公开(公告)号：EP1692297A1

    公开(公告)日：2006-08-23

    申请号：EP04798537.9

    申请日：2004-11-17

    申请人： University Of Wales, Bangor ,  TRWYN LIMITED

    发明人： KALAJI, Maher ,  WILLIAMS, Peter Anthony ,  GWENIN, Christopher David

    IPC分类号： C12Q1/00 ,  C12N9/06

    CPC分类号： C12N9/0044 ,  C12Q1/005

    摘要： This invention provides a sensing device comprising an electrode comprising a noble metal layer, on which layer is located a biological material having nitroreductase activity. This invention further provides a method of detecting nitro group containing compounds, the method comprising the steps of: (a) providing a sensing device of the first aspect of the invention and a reference electrode; (b) applying a potential between the electrodes; (c) measuring the current; (d) contacting the sensing device with a sample of substrate material to be tested; and (e) measuring the current change.

    PROMOTER VARIANTS FOR EXPRESSING GENES IN A FUNGAL CELL
    5.
    发明公开
    PROMOTER VARIANTS FOR EXPRESSING GENES IN A FUNGAL CELL 有权
    EINER PILZZELLE中PROMOTORVARIANTEN ZUR表达VON GENEN的研究

    公开(公告)号：EP1585822A4

    公开(公告)日：2005-12-28

    申请号：EP03789880

    申请日：2003-11-18

    申请人： NOVOZYMES INC

    发明人： YAVER DEBBIE ,  NHAM PETER

    IPC分类号： C12N15/80 ,  C12P1/02 ,  C12P1/00

    CPC分类号： C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

    摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

    摘要翻译： 本发明涉及生产生物物质的方法，其包括：（a）在有助于生产生物物质的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞包含可操作地编码生物物质的第一核酸序列 与包含选自SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO：6的启动子变体的第二核酸序列连接。 7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11和SEQ ID NO：12; 及其子序列; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。 本发明还涉及分离的启动子变体以及包含与编码生物物质的核酸序列可操作地连接的启动子变体的构建体，载体和真菌宿主细胞。

    TETRAHYDROFOLATE METABOLISM ENZYMES
    6.
    发明公开
    TETRAHYDROFOLATE METABOLISM ENZYMES 审中-公开
    四氢叶酸代谢酶

    公开(公告)号：EP1097222A1

    公开(公告)日：2001-05-09

    申请号：EP99935558.9

    申请日：1999-07-14

    申请人： E.I. DU PONT DE NEMOURS AND COMPANY

    发明人： FALCO, Saverio, Carl ,  FOMODU, Layo, O.

    IPC分类号： C12N15/53 ,  C12N9/06 ,  C12N5/10 ,  C12Q1/68

    CPC分类号： C12N9/0044

    摘要： This invention relates to an isolated nucleic acid fragment encoding a tetrahydrofolate metabolism enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the tetrahydrofolate metabolism enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the tetrahydrofolate metabolism enzyme in a transformed host cell.

    摘要翻译： 本发明涉及编码四氢叶酸代谢酶的分离的核酸片段。 本发明还涉及以正义或反义方向构建编码全部或部分四氢叶酸代谢酶的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的四氢叶酸代谢酶 。

    PRODUCTION OF PLANTS WITH DECREASED NITRITE CONTENT
    9.
    发明公开
    PRODUCTION OF PLANTS WITH DECREASED NITRITE CONTENT 有权
    HERSTELLUNG VON PFLANZEN MIT REDUZIERTEM NITRITGEHALT

    公开(公告)号：EP2176417A1

    公开(公告)日：2010-04-21

    申请号：EP08788678.4

    申请日：2008-08-14

    申请人： Advanced Technologies (Cambridge) Limited

    发明人： DAVENPORT, Susie ,  MAUNDERS, Martin

    IPC分类号： C12N15/82 ,  C12N15/53 ,  A01H5/00

    CPC分类号： C12N9/0044 ,  C12N15/8243

    摘要： The present invention relates in one aspect to a method for producing a transgenic plant, comprising introducing into an unmodified plant an exogenous gene encoding a nitrite reductase, wherein expression ofthe nitrite reductase encoded by the exogenous gene reduces nitrite content in the transgenic plant relative to the unmodified plant. Also provided are transgenic plants and plant cells comprising an exogenous gene encoding a nitrite reductase, as well as associated uses, chimaeric genes and plant transformation vectors.

    摘要翻译： 本发明在一个方面涉及一种生产转基因植物的方法，包括向未修饰植物中引入编码亚硝酸还原酶的外源基因，其中由外源基因编码的亚硝酸还原酶的表达降低了转基因植物中的亚硝酸盐含量，相对于 未经修改的植物。 还提供了转基因植物和植物细胞，其包含编码亚硝酸还原酶的外源基因，以及相关用途，嵌合基因和植物转化载体。