-
1.发明公开
公开(公告)号：EP1957644A2
公开(公告)日：2008-08-20
申请号：EP06818144.5
申请日：2006-12-01
申请人： Nuevolution A/S
发明人： FRANCH, Thomas , LUNDORF, Mikkel Dybro , JAKOBSEN, Søren, Nyboe , OLSEN, Eva Kampmann , ANDERSEN, Anne Lee , HOLTMANN, Anette , HANSEN, Anders Holm , SØRENSEN, Anders Malling , GOLDBECH, Anne , DE LEON, Daen , KALDOR, Ditte Kivsmose , SLØK, Frank Abilgaard , HUSEMOEN, Gitte Nystrup , DOLBERG, Johannes , JENSEN, Kim Birkebæk , PETERSEN, Lene , NØRREGAARD-MADSEN, Mads , GODSKESEN, Michael Anders , GLAD, Sanne Schrøder , NEVE, Søren , THISTED, Thomas , KRONBORG, Tine Titilola Akinleminu , SAMS, Christian, , FELDING, Jakob , FRESKGAARD, Per-Ola , GOULIAEV, Alex Haahr , PEDERSEN, Henrik
CPC分类号： C12N15/1068 , C12N15/1089 , C40B40/08 , C40B50/04 , C40B50/06
摘要： Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).
-
2.发明公开ENZYMATIC ENCODING METHODS FOR EFFICIENT SYNTHESIS OF LARGE LIBRARIES 审中-公开ENZYMVERMITTELNDE KODIERUNGSMETHODENFÜREINE EFFIZIENTE SYNTHESE VON GROSSEN BIBLIOTHEKEN
公开(公告)号：EP3018206A1
公开(公告)日：2016-05-11
申请号：EP15188924.3
申请日：2006-12-01
申请人： Nuevolution A/S
发明人： FRANCH, Thomas , LUNDORF, Mikkel Dybro , JAKOBSEN, Søren Nyboe , OLSEN, Eva Kampmann , ANDERSEN, Anne Lee , HOLTMANN, Anette , HANSEN, Anders Holm , SØRENSEN, Anders Malling , GOLDBECH, Anne , DE LEON, Dean , KALDOR, Ditte Kievsmose , SLØK, Frank Abildgaard , HUSEMOEN, Gitte Nystrup , DOLBERG, Johannes , JENSEN, Kim Birkebæk , PETERSEN, Lene , NØRREGAARD-MADSEN, Mads , GODSKESEN, Michael Anders , GLAD, Sanne Schrøder , NEVE, Søren , THISTED, Thomas , SAMS, Christian Klarner , FELDING, Jakob , KRONBERG, Tine Titilola Akinleminu , FRESKGÅRD, Per-Ola , GOLIAEV, Alex Haahr , PEDERSEN, Henrik
CPC分类号： C12N15/1068 , C12N15/1089 , C40B40/08 , C40B50/04 , C40B50/06
摘要： The present invention is in the field of split-and-mix library synthesis and is directed to a method for splitting a library synthesized by a split-and-mix synthesis strategy. The splitting of the library is performed based on nucleic acid tag sequence hybridization post library synthesis. In a first library synthesis step, nascent bifunctional complexes are dispensed in separate compartments and exposed to a different reactant in each compartment. Then, in each compartment, a different reactant reacts with at least one reactive group of a chemical reaction site of a nascent bifunctional complex, and a nucleic acid tag identifying the reactant is added by enzymatic action at a priming site of the nascent bifunctional complex. The library is then split by nucleic acid hybridization based on the sequence of different synthesis directing nucleic acid tags attached to the split-and-mix library members. The present invention combines split-and-mix library synthesis with an amplification of the synthesized library.
摘要翻译： 本发明涉及分组和混合文库合成领域，涉及一种分解通过分组合成合成策略合成的文库的方法。 文库的分裂基于文库合成后的核酸标签序列杂交进行。 在第一个文库合成步骤中，将新生的双功能复合物分配在单独的隔室中并暴露于每个隔室中的不同反应物。 然后，在每个隔室中，不同的反应物与新生的双功能复合物的化学反应位点的至少一个反应性基团反应，并且通过酶促作用在起始双功能复合物的引发位点添加鉴定反应物的核酸标签。 然后通过核酸杂交基于连接到裂解和混合文库成员的不同合成指导核酸标签的序列分离文库。 本发明将分裂和混合文库合成与合成文库的扩增相结合。
-
公开(公告)号：EP3305900A1
公开(公告)日：2018-04-11
申请号：EP17181547.5
申请日：2006-12-01
申请人： Nuevolution A/S
发明人： FRANCH, Thomas , LUNDORF, Mikkel Dybro , JAKOBSEN, Søren Nyboe , OLSEN, Eva Kampmann , ANDERSEN, Anne Lee , HOLTMANN, Anette , HANSEN, Anders Holm , SØRENSEN, Anders Malling , GOLDBECH, Anne , KALDOR, Ditte Kievsmose , DE LEON, Dean , SLØK, Frank Abildgaard , HUSEMOEN, Gitte Nystrup , DOLBERG, Johannes , JENSEN, Kim Birkebæk , PETERSEN, Lene , NØRREGAARD-MADSEN, Mads , GODSKESEN, Michael Anders , GLAD, Sanne Schrøder , NEVE, Søren , SAMS, Christian Klarner , THISTED, Thomas , GOULIAEV, Alex Haahr , KRONBERG, Tine Titiola Akinleminu , FRESKGARD, Per-Ola , PEDERSEN, Henrik , FELDING, Jakob
CPC分类号： C12N15/1068 , C12N15/1089 , C40B40/08 , C40B50/04 , C40B50/06
摘要： Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).
摘要翻译： 公开了用于获得包含与单链标识符寡核苷酸连接的分子的双功能复合物的方法，其中包含化学反应位点和用于酶促加入标签的引发位点的新生双功能复合物a）在化学反应位点与 一种或多种反应物，和b）在引发位点酶促反应，与识别反应物的一个或多个标签反应。
-
4.发明授权
公开(公告)号：EP1957644B1
公开(公告)日：2010-12-01
申请号：EP06818144.5
申请日：2006-12-01
申请人： Nuevolution A/S
发明人： FRANCH, Thomas , LUNDORF, Mikkel Dybro , JAKOBSEN, Søren , OLSEN, Eva Kampmann , ANDERSEN, Anne Lee , HOLTMANN, Anette , HANSEN, Anders Holm , SØRENSEN, Anders Malling , GOLDBECH, Anne , DE LEON, Daen , KALDOR, Ditte Kievsmose , SLØK, Frank Abilgaard , HUSEMOEN, Birgitte Nystrup , DOLBERG, Johannes , JENSEN, Kim Birkebæk , PETERSEN, Lene , NØRREGAARD-MADSEN, Mads , GODSKESEN, Michael Anders , GLAD, Sanne Schrøder , NEVE, Søren , THISTED, Thomas , KRONBORG, Tine Titilola Akinleminu , SAMS, Christian, , FELDING, Jakob , FRESKGAARD, Per-Ola , GOULIAEV, Alex Haahr , PEDERSEN, Henrik
CPC分类号： C12N15/1068 , C12N15/1089 , C40B40/08 , C40B50/04 , C40B50/06
摘要： Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).
-
-
-
搜索结果
4 条记录 (耗时 0.046 秒)
