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公开(公告)号：EP3272858A1
公开(公告)日：2018-01-24
申请号：EP16765082.9
申请日：2016-03-17
发明人： OKANO, Hideyuki , SHIOZAWA, Seiji , KISA, Fumihiko
IPC分类号： C12N5/10 , C12N5/0735 , C12N15/09
CPC分类号： C12N5/0696 , C12N5/10 , C12N15/09 , C12N2501/01 , C12N2501/20 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/605 , C12N2501/606
摘要： To produce and/or maintain naive pluripotent stem cells capable of highly expressing genes important for maintaining an undifferentiated state, which could not be achieved by known methods for producing pluripotent stem cells. The present invention can produce naive pluripotent stem cells capable of maintaining an undifferentiated state by introducing and allowing transient expression of six genes (Oct3/4, Klf4, c-Myc, Sox2, Nanog, and Klf2) among the so-called initializing factors, and further performing culturing in a medium containing LIF, an MEK inhibitor, a GSK3 inhibitor, a cAMP production promoter, a TGF-β inhibitor and a PKC inhibitor. Thus, the problem of the present invention can be solved.
摘要翻译： 为了产生和/或维持能够高度表达对维持未分化状态重要的基因的幼稚多能干细胞，这是通过用于产生多能干细胞的已知方法不能实现的。 本发明可通过在所谓的初始化因子中导入并允许六种基因（Oct3 / 4，Klf4，c-Myc，Sox2，Nanog和Klf2）的瞬时表达来产生能够维持未分化状态的幼稚多能干细胞， 并进一步在含有LIF，MEK抑制剂，GSK3抑制剂，cAMP产生促进剂，TGF-β抑制剂和PKC抑制剂的培养基中进行培养。 因此，可以解决本发明的问题。
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公开(公告)号：EP4206320A1
公开(公告)日：2023-07-05
申请号：EP21858393.8
申请日：2021-08-20
申请人： JSR Corporation , Keio University
摘要： A method of culturing human induced pluripotent stem cells includes a step of inoculating human induced pluripotent stem cells in a culture medium at an inoculation density of 1.0 × 10 4 to 1.0 × 10 6 cells/cm 2 in a culture vessel and subjecting the human induced pluripotent stem cells to two-dimensional culturing. A method of producing cerebral organoids includes a step 1 of culturing a culture of the human induced pluripotent stem cells obtained by the method of culturing human induced pluripotent stem cells in a culture medium containing a BMP inhibitor and a transforming growth factor β (TGFβ) inhibitor to form cell aggregates, a step 2 of culturing the cell aggregates in a culture medium containing a Wnt signal transduction pathway potentiator and an extracellular matrix, and a step 3 of subjecting the culturing obtained in the step 2 to spinner culturing.
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公开(公告)号：EP4056683A1
公开(公告)日：2022-09-14
申请号：EP20886120.3
申请日：2020-11-05
申请人： JSR Corporation , Keio University
摘要： According to a production method for a brain organoid, comprising a step 1 of carrying out suspension culture of human pluripotent stem cells having a mutation in at least one or more base sequences in an exon selected from the group consisting of an exon 9, an exon 10, an exon 11, an exon 12, and an exon 13 of a microtubule-associated protein tau (MAPT) gene, and having a mutation in at least one or more base sequences in an intron 10 of the MAPT gene, it is possible to produce a brain organoid having a phosphorylated 3-repeat tau protein and a phosphorylated 4-repeat tau protein.
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公开(公告)号：EP3957722A1
公开(公告)日：2022-02-23
申请号：EP20792193.3
申请日：2020-04-17
申请人： Keio University , Nihon University
摘要： This method of producing induced pluripotent stem cells involves a step for introducing an initialization factor into somatic cells of a mammal, and culturing in a neural stem cell culture medium to obtain induced neural stem cell-like cells, and a step for cultivating said induced neural stem cell-like cells in a growth medium to obtain induced pluripotent stem cells, wherein the initialization factor contains an OCT family, a SOX family, a KLF family, a MYC family, a LIN28 family and a P53 function inhibitor.
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