公开(公告)号：EP4194801A1

公开(公告)日：2023-06-14

申请号：EP23150895.3

申请日：2016-02-24

申请人： The University of Tokyo ,  OSAKA UNIVERSITY

发明人： OTA, Sadao ,  HORISAKI, Ryoichi ,  HASHIMOTO, Kazuki

IPC分类号： G01B11/00 ,  G01N21/01 ,  G01N21/64 ,  H03M7/30

摘要： Any one or both of an optical system with a structured lighting pattern and a structured detecting system having a plurality of regions with different optical characteristics are used. In addition, optical signals from an object to be observed through one or a small number of pixel detectors are detected while changing relative positions between the object to be observed and any one of the optical system and the detecting system, time series signal information of the optical signals are obtained, and an image associated with an object to be observed from the time series signal information is reconstructed.

公开(公告)号：EP4306931A3

公开(公告)日：2024-02-07

申请号：EP23195563.4

申请日：2019-06-12

申请人： ThinkCyte K.K. ,  University of Tokyo ,  Osaka University

发明人： OTA, Sadao ,  HORISAKI, Ryoichi ,  KAWAMURA, Yoko ,  UGAWA, Masashi ,  SATO, Issei

IPC分类号： G01N15/14

摘要： The present disclosure provides methods and systems for ghost cytometry (GC), which may be used to produce an image of an object without using a spatially resolving detector. This may be used to perform image-free ultrafast fluorescence "imaging" cytometry, based on, for example, a single pixel detector. Spatial information obtained from the motion of cells relative to a patterned optical structure may be compressively converted into signals that arrive sequentially at a single pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random or pseudo-random pattern may permit computational reconstruction of cell morphology. Machine learning methods may be applied directly to the compressed waveforms without image reconstruction to enable efficient image-free morphology-based cytometry. Image-free GC may achieve accurate and high throughput cell classification as well as selective sorting based on cell morphology without a specific biomarker, which have been challenging using conventional flow cytometers.

公开(公告)号：EP3822685A1

公开(公告)日：2021-05-19

申请号：EP19835002.7

申请日：2019-07-12

申请人： The University of Tokyo ,  Osaka University

发明人： IDEGUCHI, Takuro ,  TODA, Keiichiro ,  TAMAMITSU, Miu ,  HASHIMOTO, Kazuki ,  HORISAKI, Ryoichi

IPC分类号： G02B21/06 ,  G01N21/17 ,  G01N21/21 ,  G01N21/41 ,  G02B21/14 ,  G02B21/36

摘要： Irradiation light in a visible light region is irradiated to a sample while switching irradiation of infrared light IR having a wavelength that corresponds to the infrared absorption spectrum of an observation target material included in the sample between a first state and a second state. A first image and a second image are generated based on the phase distribution, the intensity distribution, and the polarization direction distribution of the light including the irradiation light that has passed through the sample in synchronization with the switching of the infrared light IR irradiation between the first state and the second state. Subsequently, an output image is generated so as to represent one from among the position, size, and shape based on the difference and/or ratio with respect to the pixel values for each pixel between the first image and the second image.

公开(公告)号：EP3807005A1

公开(公告)日：2021-04-21

申请号：EP19819019.1

申请日：2019-06-12

申请人： ThinkCyte, Inc. ,  University of Tokyo ,  Osaka University

发明人： OTA, Sadao ,  HORISAKI, Ryoichi ,  KAWAMURA, Yoko ,  UGAWA, Masashi ,  SATO, Issei

IPC分类号： B01L3/00 ,  G01N15/10

公开(公告)号：EP3264031A1

公开(公告)日：2018-01-03

申请号：EP16755545.7

申请日：2016-02-24

申请人： The University of Tokyo ,  Osaka University

发明人： OTA, Sadao ,  HORISAKI, Ryoichi ,  HASHIMOTO, Kazuki

IPC分类号： G01B11/00 ,  G01N21/01 ,  G01N21/64

CPC分类号： G01N21/01 ,  G01B11/00 ,  G01N21/53 ,  G01N21/59 ,  G01N21/64 ,  G01N21/6456 ,  H03M7/3062

摘要： Any one or both of an optical system with a structured lighting pattern and a structured detecting system having a plurality of regions with different optical characteristics are used. In addition, optical signals from an object to be observed through one or a small number of pixel detectors are detected while changing relative positions between the object to be observed and any one of the optical system and the detecting system, time series signal information of the optical signals are obtained, and an image associated with an object to be observed from the time series signal information is reconstructed.

摘要翻译： 使用具有结构化照明图案的光学系统和具有多个具有不同光学特性的区域的结构化检测系统中的任何一个或两者。 另外，在改变被观察对象与光学系统和检测系统中的任何一个之间的相对位置的同时，检测来自要通过一个或少数像素检测器观察的对象的光学信号，该时间序列信号信息 获得光学信号，并且重构与时间序列信号信息中的要观察的对象相关联的图像。

公开(公告)号：EP4306931A2

公开(公告)日：2024-01-17

申请号：EP23195563.4

申请日：2019-06-12

申请人： ThinkCyte K.K. ,  University of Tokyo ,  Osaka University

发明人： OTA, Sadao ,  HORISAKI, Ryoichi ,  KAWAMURA, Yoko ,  UGAWA, Masashi ,  SATO, Issei

IPC分类号： G01N15/14

摘要： The present disclosure provides methods and systems for ghost cytometry (GC), which may be used to produce an image of an object without using a spatially resolving detector. This may be used to perform image-free ultrafast fluorescence "imaging" cytometry, based on, for example, a single pixel detector. Spatial information obtained from the motion of cells relative to a patterned optical structure may be compressively converted into signals that arrive sequentially at a single pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random or pseudo-random pattern may permit computational reconstruction of cell morphology. Machine learning methods may be applied directly to the compressed waveforms without image reconstruction to enable efficient image-free morphology-based cytometry. Image-free GC may achieve accurate and high throughput cell classification as well as selective sorting based on cell morphology without a specific biomarker, which have been challenging using conventional flow cytometers.

公开(公告)号：EP4160319A1

公开(公告)日：2023-04-05

申请号：EP22203951.3

申请日：2017-08-10

申请人： OSAKA UNIVERSITY

发明人： HORISAKI, Ryoichi ,  TANIDA, Jun ,  EGAMI, Riki

IPC分类号： G03H1/04 ,  G03H1/08 ,  H03M7/30 ,  G01N21/17 ,  G01J9/00

摘要： An electromagnetic wave phase/amplitude generation device includes a radiation unit configured to radiate electromagnetic waves of a random radiation pattern on a spatial frequency in which a state of the electromagnetic waves to be radiated for each divided region is determined to an imaging object, an imaging unit configured to generate a captured image by imaging scattered electromagnetic waves that are electromagnetic waves generated when the imaging object scatters the electromagnetic waves of the radiation pattern radiated by the radiation unit, and a generation unit configured to generate information indicating at least a phase and amplitude of the electromagnetic waves from the imaging object by performing an arithmetic sparsity constraint operation according to sparsity of the imaging object on the basis of the captured image generated by the imaging unit, information indicating the radiation pattern, and information indicating a signal of the imaging object.

公开(公告)号：EP3584564A1

公开(公告)日：2019-12-25

申请号：EP18753550.5

申请日：2018-02-15

申请人： Osaka University ,  The University Of Tokyo

发明人： HORISAKI, Ryoichi ,  TANIDA, Jun ,  OTA, Sadao

IPC分类号： G01N21/45 ,  G01N15/14

摘要： An electromagnetic wave detecting device comprising: an emission unit configured to emit electromagnetic waves having coherence; an electromagnetic wave modulating unit configured to modulate one or both of a phase and an amplitude of the emitted electromagnetic waves and to change a state of the modulation relative to an imaging target; and a post-modulation electromagnetic wave intensity detecting unit configured to detect an intensity of post-modulation electromagnetic waves, which are the modulated electromagnetic waves acquired by modulating the electromagnetic waves emitted from the emission unit using the imaging target and the electromagnetic wave modulating unit, using one pixel.