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公开(公告)号：EP3440098A1
公开(公告)日：2019-02-13
申请号：EP17722128.0
申请日：2017-04-06
发明人： JAYASINGHE, Lakmal , BRUCE, Mark , MCNEILL, Luke , NATHANI, Ramiz Iqbal , SINGH, Pratik Raj , WOOD, Neil Roger , YOUNG, Stephen Robert
IPC分类号： C07K14/435 , C12Q1/68 , G01N27/447
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公开(公告)号：EP2875128B8
公开(公告)日：2020-06-24
申请号：EP13740348.1
申请日：2013-07-18
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公开(公告)号：EP2875152B1
公开(公告)日：2019-10-09
申请号：EP13740351.5
申请日：2013-07-18
发明人： HERON, Andrew , CLARKE, James , MOYSEY, Ruth , WALLACE, Elizabeth Jayne , BRUCE, Mark , JAYASINGHE, Lakmal , CAPROTTI, Domenico , SOEROES, Szabolcs , MCNEILL, Luke , MISCA, Mihaela
IPC分类号： C12Q1/6869 , C12N9/14
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公开(公告)号：EP2875152A1
公开(公告)日：2015-05-27
申请号：EP13740351.5
申请日：2013-07-18
发明人： HERON, Andrew , CLARKE, James , MOYSEY, Ruth , WALLACE, Elizabeth Jayne , BRUCE, Mark , JAYASINGHE, Lakmal , CAPROTTI, Domenico , SOEROES, Szabolcs , MCNEILL, Luke , MISCA, Mihaela
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C07K2319/00 , C12N9/14 , C12Y306/04 , C12Q2521/513 , C12Q2522/101 , C12Q2565/631
摘要： The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
摘要翻译： 本发明涉及使用包含解旋酶和另外的多核苷酸结合部分的构建体的方法。 解旋酶连接到多核苷酸结合部分，并且构建体具有控制多核苷酸运动的能力。 该构建体可以用于控制多核苷酸的运动，并且对于测序多核苷酸特别有用。
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公开(公告)号：EP2875128A1
公开(公告)日：2015-05-27
申请号：EP13740348.1
申请日：2013-07-18
发明人： HERON, Andrew , CLARKE, James , MOYSEY, Ruth , WALLACE, Elizabeth Jayne , BRUCE, Mark , JAYASINGHE, Lakmal , CAPROTTI, Domenico , SOEROES, Szabolcs , MCNEILL, Luke , ALVES, David , BOWEN, Rebecca , MILTON, John
摘要： The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
摘要翻译： 本发明涉及具有减少的来自多核苷酸的结合的修饰的解旋酶。 解旋酶可用于控制多核苷酸的运动，并且特别可用于测序多核苷酸。
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公开(公告)号：EP2391655A1
公开(公告)日：2011-12-07
申请号：EP10705403.3
申请日：2010-01-29
发明人： JAYASINGHE, Lakmal , MILTON, John , MCNEILL, Luke , CLARKE, James , WHITE, James , MOYSEY, Ruth
CPC分类号： C12N9/22 , C07K14/31 , C07K2319/03 , C12N9/1241 , C12N9/90
摘要： The invention provides method of covalently coupling two or more moieties, the method comprising: (a) providing a first moiety having covalently attached thereto (i) at least one first linker comprising a first hybridizable region and (ii) at least one first group capable of forming a covalent bond; (b) providing a second moiety having covalently attached thereto (i) at least one second linker comprising a second hybridizable region capable of hybridizing to the first hybridizable region and (ii) at least a second group capable of forming a covalent bond with the first group; (c) contacting the first and second moieties under conditions that allow the first and second hybridizable regions to hybridize and link the moieties; and (d) exposing the linked moieties to conditions that allow the formation of a covalent bond between the first and second groups.
摘要翻译： 本发明涉及包含核酸结合蛋白和表面的构建体。 从结合蛋白中去除至少一种可天然存在的半胱氨酸残基。 结合蛋白通过一个或多个可接近的半胱氨酸残基连接到表面。 从蛋白质中去除其他可获得的半胱氨酸残基允许控制附着于表面。 构建体可用于产生具有与其连接的核酸结合蛋白的跨膜孔。 这样的孔特别可用于测序核酸。 酶以这样的方式处理核酸，使得孔可以通过随机感测来检测其每个组分核苷酸。
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公开(公告)号：EP3741847A1
公开(公告)日：2020-11-25
申请号：EP20171363.3
申请日：2013-07-18
发明人： HERON, Andrew , CLARKE, James , MOYSEY, Ruth , WALLACE, Elizabeth Jayne , BRUCE, Mark , JAYASINGHE, Lakmal , CAPROTTI, Domenico , SOEROES, Szabolcs , MCNEILL, Luke , ALVES, David , BOWEN, Rebecca , MILTON, John
摘要： The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
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公开(公告)号：EP3097204B1
公开(公告)日：2020-07-08
申请号：EP14776897.2
申请日：2014-09-10
发明人： BOWEN, Rebecca Victoria , BROWN, Clive Gavin , BRUCE, Mark , HERON, Andrew John , WALLACE, Elizabeth Jayne , WHITE, James , LLOYD, Joseph Hargreaves , ALVES, David Antoni , CAPROTTI, Domenico , JAYASINGHE, Lakmal , MCNEILL, Luke , MILTON, John , PUGLISI, Antonino , SOEROES, Szabolcs
IPC分类号： C12Q1/6869
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公开(公告)号：EP2391655B1
公开(公告)日：2017-10-11
申请号：EP10705403.3
申请日：2010-01-29
发明人： JAYASINGHE, Lakmal , MILTON, John , MCNEILL, Luke , CLARKE, James , WHITE, James , MOYSEY, Ruth
CPC分类号： C12N9/22 , C07K14/31 , C07K2319/03 , C12N9/1241 , C12N9/90
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10.发明公开METHOD FOR CONTROLLING THE MOVEMENT OF A POLYNUCLEOTIDE THROUGH A TRANSMEMBRANE PORE 审中-公开VERFAHREN ZUR STEUERUNG DER BEWEGUNG EINES POLYNUKLEOTIDS DURCH EINE TRANSMEMBRANPORE
公开(公告)号：EP3097204A1
公开(公告)日：2016-11-30
申请号：EP14776897.2
申请日：2014-09-10
发明人： BOWEN, Rebecca Victoria , BROWN, Clive Gavin , BRUCE, Mark , HERON, Andrew John , WALLACE, Elizabeth Jayne , WHITE, James , LLOYD, Joseph Hargreaves , ALVES, David Antoni , CAPROTTI, Domenico , JAYASINGHE, Lakmal , MCNEILL, Luke , MILTON, John , PUGLISI, Antonino , SOEROES, Szabolcs
IPC分类号： C12Q1/68 , G01N33/487
摘要： The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterising target polynucleotides using helicases.
摘要翻译： 本发明涉及控制多核苷酸通过跨膜孔运动的新方法。 本发明还涉及使用解旋酶表征靶多核苷酸的新方法。
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