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    METHOD OF CELL CULTURE
    1.
    发明公开
    METHOD OF CELL CULTURE 审中-公开
    VERFAHREN ZUR ZELLKULTUR

    公开(公告)号：EP3119876A1

    公开(公告)日：2017-01-25

    申请号：EP15715840.3

    申请日：2015-03-17

    申请人： Pfizer Inc.

    发明人： HILLER, Gregory Walter ,  MULUKUTLA, Bhanu Chandra

    IPC分类号： C12N5/00

    CPC分类号： C12P21/02 ,  C12N5/0018 ,  C12N5/0682 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/34 ,  C12N2501/80 ,  C12N2501/999

    摘要： A method of cell culture comprising providing cells in a cell culture medium to start a cell culture process, and, maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4- hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2- hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3mM and/or (ii) maintaining at least one amino acid selected from phenylalanine, tyrosine, tryptophan, methionine, leucine, serine, threonine and glycine below a concentration C2 in the cell culture medium, wherein C2 is 2mM.

    摘要翻译： 一种细胞培养方法，包括在细胞培养基中提供细胞以开始细胞培养过程，以及保持至少一种选自3-（4-羟基苯基）乳酸盐，4-羟基苯基丙酮酸盐，苯基乳酸盐，吲哚乳酸盐，吲哚羧酸，同型半胱氨酸的代谢物 ，2-羟基丁酸，异戊酸盐和低于浓度C1的甲酸盐，其中C1为3mM和/或（ii）保持至少一种选自苯丙氨酸，酪氨酸，色氨酸，甲硫氨酸，亮氨酸，丝氨酸，苏氨酸的氨基酸 和低于细胞培养基中C2浓度的甘氨酸，其中C2为2mM。

    CELLS AND METHOD OF CELL CULTURE
    4.
    发明公开
    CELLS AND METHOD OF CELL CULTURE 审中-公开

    公开(公告)号：EP4446338A2

    公开(公告)日：2024-10-16

    申请号：EP24190440.8

    申请日：2016-09-22

    申请人： Pfizer Inc.

    发明人： HILLER, Gregory Walter ,  MITCHELL, Jeffrey Joseph ,  MULUKUTLA, Bhanu Chandra ,  PEGMAN, Pamela Mary

    IPC分类号： C07K14/435

    摘要： The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.